Experiment: 3-methyl-3-butenol 0.31 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymfD and ymfE are separated by 0 nucleotides ymfE and lit are separated by 457 nucleotides
b1137: ymfD - e14 prophage; predicted SAM-dependent methyltransferase (NCBI), at 1,196,090 to 1,196,755
ymfD
b1138: ymfE - e14 prophage; predicted inner membrane protein (NCBI), at 1,196,756 to 1,197,460
ymfE
b1139: lit - e14 prophage; cell death peptidase, inhibitor of T4 late gene expression (NCBI), at 1,197,918 to 1,198,811
lit
Position (kb)
1196
1197
1198 Strain fitness (log2 ratio)
-1
0
1
2 at 1195.760 kb on + strand at 1195.807 kb on + strand at 1195.888 kb on + strand at 1195.894 kb on - strand at 1195.894 kb on - strand at 1195.894 kb on - strand at 1195.895 kb on - strand at 1195.895 kb on - strand at 1195.896 kb on - strand at 1195.896 kb on - strand at 1195.896 kb on - strand at 1195.932 kb on + strand at 1195.932 kb on + strand at 1195.938 kb on - strand at 1195.941 kb on - strand at 1195.944 kb on + strand at 1195.944 kb on + strand at 1195.954 kb on + strand at 1195.957 kb on - strand at 1195.961 kb on - strand at 1195.963 kb on - strand at 1195.972 kb on - strand at 1195.982 kb on - strand at 1195.985 kb on + strand at 1196.004 kb on - strand at 1196.045 kb on + strand at 1196.045 kb on + strand at 1196.054 kb on + strand at 1196.105 kb on - strand at 1196.127 kb on - strand at 1197.326 kb on - strand, within ymfE at 1197.530 kb on - strand at 1197.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol 0.31 vol% remove 1,195,760 + +0.7 1,195,807 + +0.0 1,195,888 + +0.2 1,195,894 - -0.2 1,195,894 - +0.8 1,195,894 - +1.1 1,195,895 - -0.1 1,195,895 - -1.2 1,195,896 - +1.1 1,195,896 - +0.4 1,195,896 - +1.8 1,195,932 + +0.3 1,195,932 + +2.2 1,195,938 - +0.1 1,195,941 - +0.3 1,195,944 + +0.6 1,195,944 + +0.1 1,195,954 + -1.4 1,195,957 - +0.2 1,195,961 - +0.1 1,195,963 - +0.6 1,195,972 - -1.4 1,195,982 - -0.6 1,195,985 + +0.1 1,196,004 - -0.0 1,196,045 + +0.3 1,196,045 + +0.1 1,196,054 + +0.1 1,196,105 - +0.2 1,196,127 - +0.2 1,197,326 - ymfE b1138 0.81 -0.6 1,197,530 - +0.4 1,197,781 + +0.2
Or see this region's nucleotide sequence