Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2916 and Psyr_2917 are separated by 73 nucleotides Psyr_2917 and Psyr_2918 are separated by 43 nucleotides
Psyr_2916: Psyr_2916 - Substrate-binding region of ABC-type glycine betaine transport system, at 3,504,820 to 3,505,764
_2916
Psyr_2917: Psyr_2917 - Prokaryotic protein of unknown function DUF849, at 3,505,838 to 3,506,725
_2917
Psyr_2918: Psyr_2918 - 3-hydroxyacyl-CoA dehydrogenase, C-terminal:3-hydroxyacyl-CoA dehydrogenase, NAD-binding protein, at 3,506,769 to 3,507,725
_2918
Position (kb)
3505
3506
3507 Strain fitness (log2 ratio)
-1
0
1
2 at 3504.884 kb on + strand at 3504.885 kb on - strand at 3504.929 kb on + strand, within Psyr_2916 at 3504.930 kb on - strand, within Psyr_2916 at 3504.994 kb on + strand, within Psyr_2916 at 3505.189 kb on + strand, within Psyr_2916 at 3505.189 kb on + strand, within Psyr_2916 at 3505.189 kb on + strand, within Psyr_2916 at 3505.189 kb on + strand, within Psyr_2916 at 3505.465 kb on + strand, within Psyr_2916 at 3505.465 kb on + strand, within Psyr_2916 at 3505.466 kb on - strand, within Psyr_2916 at 3505.466 kb on - strand, within Psyr_2916 at 3505.826 kb on - strand at 3505.908 kb on + strand at 3506.258 kb on - strand, within Psyr_2917 at 3506.258 kb on - strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.261 kb on + strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.262 kb on - strand, within Psyr_2917 at 3506.710 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.739 kb on + strand at 3506.740 kb on - strand at 3506.777 kb on + strand at 3507.109 kb on + strand, within Psyr_2918 at 3507.110 kb on - strand, within Psyr_2918 at 3507.122 kb on + strand, within Psyr_2918 at 3507.123 kb on - strand, within Psyr_2918 at 3507.207 kb on + strand, within Psyr_2918 at 3507.246 kb on - strand, within Psyr_2918 at 3507.246 kb on - strand, within Psyr_2918 at 3507.282 kb on + strand, within Psyr_2918 at 3507.282 kb on + strand, within Psyr_2918 at 3507.283 kb on - strand, within Psyr_2918 at 3507.361 kb on + strand, within Psyr_2918 at 3507.477 kb on + strand, within Psyr_2918 at 3507.555 kb on + strand, within Psyr_2918 at 3507.556 kb on - strand, within Psyr_2918 at 3507.556 kb on - strand, within Psyr_2918 at 3507.634 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.635 kb on + strand at 3507.636 kb on - strand at 3507.636 kb on - strand at 3507.636 kb on - strand at 3507.699 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 3,504,884 + -1.2 3,504,885 - +0.4 3,504,929 + Psyr_2916 0.12 -1.6 3,504,930 - Psyr_2916 0.12 +0.1 3,504,994 + Psyr_2916 0.18 -1.3 3,505,189 + Psyr_2916 0.39 -0.2 3,505,189 + Psyr_2916 0.39 -0.3 3,505,189 + Psyr_2916 0.39 +1.2 3,505,189 + Psyr_2916 0.39 -0.7 3,505,465 + Psyr_2916 0.68 +1.1 3,505,465 + Psyr_2916 0.68 +0.3 3,505,466 - Psyr_2916 0.68 -0.3 3,505,466 - Psyr_2916 0.68 -0.4 3,505,826 - -0.9 3,505,908 + -0.5 3,506,258 - Psyr_2917 0.47 +0.8 3,506,258 - Psyr_2917 0.47 +0.6 3,506,261 + Psyr_2917 0.48 -0.7 3,506,261 + Psyr_2917 0.48 +1.2 3,506,261 + Psyr_2917 0.48 +1.1 3,506,261 + Psyr_2917 0.48 +0.3 3,506,261 + Psyr_2917 0.48 -0.8 3,506,262 - Psyr_2917 0.48 +0.6 3,506,262 - Psyr_2917 0.48 -0.3 3,506,262 - Psyr_2917 0.48 +0.5 3,506,262 - Psyr_2917 0.48 +0.0 3,506,262 - Psyr_2917 0.48 -0.2 3,506,262 - Psyr_2917 0.48 -1.2 3,506,262 - Psyr_2917 0.48 +0.9 3,506,262 - Psyr_2917 0.48 +0.4 3,506,262 - Psyr_2917 0.48 +0.9 3,506,262 - Psyr_2917 0.48 -0.2 3,506,262 - Psyr_2917 0.48 -0.0 3,506,262 - Psyr_2917 0.48 +0.3 3,506,262 - Psyr_2917 0.48 +0.2 3,506,262 - Psyr_2917 0.48 +0.4 3,506,710 + +0.4 3,506,739 + +0.1 3,506,739 + +0.7 3,506,739 + +0.8 3,506,739 + -0.1 3,506,740 - -1.1 3,506,777 + -0.0 3,507,109 + Psyr_2918 0.36 +0.4 3,507,110 - Psyr_2918 0.36 +0.5 3,507,122 + Psyr_2918 0.37 +1.5 3,507,123 - Psyr_2918 0.37 +0.8 3,507,207 + Psyr_2918 0.46 -0.6 3,507,246 - Psyr_2918 0.50 -0.1 3,507,246 - Psyr_2918 0.50 -0.9 3,507,282 + Psyr_2918 0.54 +0.4 3,507,282 + Psyr_2918 0.54 +1.9 3,507,283 - Psyr_2918 0.54 -0.1 3,507,361 + Psyr_2918 0.62 +1.0 3,507,477 + Psyr_2918 0.74 +1.8 3,507,555 + Psyr_2918 0.82 +1.4 3,507,556 - Psyr_2918 0.82 +0.1 3,507,556 - Psyr_2918 0.82 +0.1 3,507,634 + +0.4 3,507,635 + -0.7 3,507,635 + -0.3 3,507,635 + -1.6 3,507,635 + +0.6 3,507,635 + -0.7 3,507,635 + +1.1 3,507,635 + +0.2 3,507,635 + +0.4 3,507,636 - +0.8 3,507,636 - -0.1 3,507,636 - -0.8 3,507,699 - +0.2
Or see this region's nucleotide sequence