Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2869 and Psyr_2870 are separated by 4 nucleotides Psyr_2870 and Psyr_2871 are separated by 15 nucleotides Psyr_2871 and Psyr_2872 are separated by 38 nucleotides Psyr_2872 and Psyr_2873 are separated by 148 nucleotides
Psyr_2869: Psyr_2869 - xenobiotic compound monooxygenase, DszA family, at 3,443,600 to 3,445,000
_2869
Psyr_2870: Psyr_2870 - CDS, at 3,445,005 to 3,445,445
_2870
Psyr_2871: Psyr_2871 - CDS, at 3,445,461 to 3,446,675
_2871
Psyr_2872: Psyr_2872 - hypothetical protein, at 3,446,714 to 3,446,953
_2872
Psyr_2873: Psyr_2873 - Acyl-CoA dehydrogenase, C-terminal, at 3,447,102 to 3,448,340
_2873
Position (kb)
3445
3446
3447 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3444.496 kb on - strand, within Psyr_2869 at 3444.510 kb on + strand, within Psyr_2869 at 3444.511 kb on - strand, within Psyr_2869 at 3444.588 kb on + strand, within Psyr_2869 at 3444.804 kb on + strand, within Psyr_2869 at 3444.813 kb on + strand, within Psyr_2869 at 3444.813 kb on + strand, within Psyr_2869 at 3444.814 kb on - strand, within Psyr_2869 at 3444.814 kb on - strand, within Psyr_2869 at 3444.904 kb on - strand at 3444.904 kb on - strand at 3444.904 kb on - strand at 3444.904 kb on - strand at 3445.000 kb on + strand at 3445.000 kb on + strand at 3445.000 kb on + strand at 3445.000 kb on + strand at 3445.000 kb on + strand at 3445.000 kb on + strand at 3445.000 kb on + strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.001 kb on - strand at 3445.006 kb on + strand at 3445.006 kb on + strand at 3445.006 kb on + strand at 3445.006 kb on + strand at 3445.007 kb on - strand at 3445.007 kb on - strand at 3445.136 kb on + strand, within Psyr_2870 at 3445.137 kb on - strand, within Psyr_2870 at 3445.294 kb on + strand, within Psyr_2870 at 3445.427 kb on + strand at 3445.451 kb on + strand at 3445.452 kb on - strand at 3445.812 kb on - strand, within Psyr_2871 at 3446.022 kb on + strand, within Psyr_2871 at 3446.023 kb on - strand, within Psyr_2871 at 3446.023 kb on - strand, within Psyr_2871 at 3446.317 kb on + strand, within Psyr_2871 at 3446.317 kb on + strand, within Psyr_2871 at 3446.317 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.446 kb on + strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.447 kb on - strand, within Psyr_2871 at 3446.467 kb on + strand, within Psyr_2871 at 3446.888 kb on + strand, within Psyr_2872 at 3447.096 kb on + strand at 3447.573 kb on + strand, within Psyr_2873 at 3447.574 kb on - strand, within Psyr_2873 at 3447.574 kb on - strand, within Psyr_2873 at 3447.574 kb on - strand, within Psyr_2873 at 3447.574 kb on - strand, within Psyr_2873 at 3447.576 kb on + strand, within Psyr_2873 at 3447.576 kb on + strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873 at 3447.577 kb on - strand, within Psyr_2873
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 3,444,496 - Psyr_2869 0.64 +0.1 3,444,510 + Psyr_2869 0.65 -2.1 3,444,511 - Psyr_2869 0.65 +0.6 3,444,588 + Psyr_2869 0.71 -0.7 3,444,804 + Psyr_2869 0.86 -1.1 3,444,813 + Psyr_2869 0.87 -0.7 3,444,813 + Psyr_2869 0.87 -0.5 3,444,814 - Psyr_2869 0.87 -2.1 3,444,814 - Psyr_2869 0.87 -0.7 3,444,904 - +1.5 3,444,904 - -0.2 3,444,904 - +0.5 3,444,904 - +0.4 3,445,000 + +1.4 3,445,000 + +0.5 3,445,000 + -0.4 3,445,000 + -0.2 3,445,000 + +0.7 3,445,000 + -0.6 3,445,000 + -1.4 3,445,001 - +0.7 3,445,001 - -0.8 3,445,001 - -0.6 3,445,001 - -0.8 3,445,001 - -0.5 3,445,001 - +0.7 3,445,001 - +0.9 3,445,001 - +0.6 3,445,001 - +0.2 3,445,001 - +0.4 3,445,001 - -2.4 3,445,006 + -0.7 3,445,006 + +0.9 3,445,006 + +0.9 3,445,006 + -0.7 3,445,007 - -0.6 3,445,007 - +0.9 3,445,136 + Psyr_2870 0.30 -1.1 3,445,137 - Psyr_2870 0.30 +0.8 3,445,294 + Psyr_2870 0.66 -0.6 3,445,427 + -0.2 3,445,451 + -0.7 3,445,452 - +0.1 3,445,812 - Psyr_2871 0.29 +0.2 3,446,022 + Psyr_2871 0.46 -0.6 3,446,023 - Psyr_2871 0.46 +1.0 3,446,023 - Psyr_2871 0.46 +0.2 3,446,317 + Psyr_2871 0.70 +1.2 3,446,317 + Psyr_2871 0.70 +1.5 3,446,317 + Psyr_2871 0.70 -1.1 3,446,446 + Psyr_2871 0.81 -1.1 3,446,446 + Psyr_2871 0.81 -0.2 3,446,446 + Psyr_2871 0.81 -0.4 3,446,447 - Psyr_2871 0.81 +2.8 3,446,447 - Psyr_2871 0.81 -0.3 3,446,447 - Psyr_2871 0.81 -0.6 3,446,467 + Psyr_2871 0.83 -0.4 3,446,888 + Psyr_2872 0.72 -0.4 3,447,096 + -0.6 3,447,573 + Psyr_2873 0.38 -0.0 3,447,574 - Psyr_2873 0.38 -0.1 3,447,574 - Psyr_2873 0.38 -1.2 3,447,574 - Psyr_2873 0.38 +0.9 3,447,574 - Psyr_2873 0.38 +0.4 3,447,576 + Psyr_2873 0.38 +0.7 3,447,576 + Psyr_2873 0.38 +0.2 3,447,577 - Psyr_2873 0.38 -1.1 3,447,577 - Psyr_2873 0.38 -2.0
Or see this region's nucleotide sequence