Experiment: KB with Rotenone 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0492 and Psyr_0493 overlap by 8 nucleotides Psyr_0493 and Psyr_0494 are separated by 121 nucleotides Psyr_0494 and Psyr_0495 are separated by 138 nucleotides
Psyr_0492: Psyr_0492 - Response regulator receiver:CheW-like protein:ATP-binding region, ATPase-like:Hpt, at 528,991 to 534,969
_0492
Psyr_0493: Psyr_0493 - CheW-like protein, at 534,962 to 535,432
_0493
Psyr_0494: Psyr_0494 - Protein of unknown function UPF0061, at 535,554 to 537,017
_0494
Psyr_0495: Psyr_0495 - transcriptional regulator, GntR family, at 537,156 to 537,905
_0495
Position (kb)
535
536
537
538 Strain fitness (log2 ratio)
-1
0
1
2 at 534.898 kb on + strand at 534.898 kb on + strand at 534.947 kb on - strand at 535.115 kb on + strand, within Psyr_0493 at 535.313 kb on + strand, within Psyr_0493 at 535.313 kb on + strand, within Psyr_0493 at 535.313 kb on + strand, within Psyr_0493 at 535.313 kb on + strand, within Psyr_0493 at 535.314 kb on - strand, within Psyr_0493 at 535.314 kb on - strand, within Psyr_0493 at 535.378 kb on + strand, within Psyr_0493 at 535.430 kb on + strand at 535.430 kb on + strand at 535.435 kb on - strand at 535.486 kb on + strand at 535.486 kb on + strand at 535.735 kb on + strand, within Psyr_0494 at 535.736 kb on - strand, within Psyr_0494 at 535.736 kb on - strand, within Psyr_0494 at 535.802 kb on + strand, within Psyr_0494 at 535.803 kb on - strand, within Psyr_0494 at 535.814 kb on - strand, within Psyr_0494 at 535.921 kb on + strand, within Psyr_0494 at 535.922 kb on - strand, within Psyr_0494 at 535.922 kb on - strand, within Psyr_0494 at 536.026 kb on + strand, within Psyr_0494 at 536.027 kb on - strand, within Psyr_0494 at 536.039 kb on - strand, within Psyr_0494 at 536.240 kb on + strand, within Psyr_0494 at 536.444 kb on - strand, within Psyr_0494 at 536.444 kb on - strand, within Psyr_0494 at 536.444 kb on - strand, within Psyr_0494 at 536.452 kb on + strand, within Psyr_0494 at 536.452 kb on + strand, within Psyr_0494 at 536.716 kb on + strand, within Psyr_0494 at 536.717 kb on - strand, within Psyr_0494 at 536.764 kb on + strand, within Psyr_0494 at 536.764 kb on + strand, within Psyr_0494 at 536.765 kb on - strand, within Psyr_0494 at 536.765 kb on - strand, within Psyr_0494 at 536.765 kb on - strand, within Psyr_0494 at 536.788 kb on + strand, within Psyr_0494 at 536.788 kb on + strand, within Psyr_0494 at 536.788 kb on + strand, within Psyr_0494 at 536.914 kb on - strand at 537.128 kb on - strand at 537.292 kb on + strand, within Psyr_0495 at 537.293 kb on - strand, within Psyr_0495 at 537.293 kb on - strand, within Psyr_0495 at 537.364 kb on + strand, within Psyr_0495 at 537.365 kb on - strand, within Psyr_0495 at 537.566 kb on - strand, within Psyr_0495 at 537.566 kb on - strand, within Psyr_0495 at 537.587 kb on + strand, within Psyr_0495 at 537.651 kb on + strand, within Psyr_0495 at 537.651 kb on + strand, within Psyr_0495 at 537.674 kb on + strand, within Psyr_0495 at 537.674 kb on + strand, within Psyr_0495 at 537.804 kb on - strand, within Psyr_0495 at 537.804 kb on - strand, within Psyr_0495 at 537.804 kb on - strand, within Psyr_0495 at 537.804 kb on - strand, within Psyr_0495 at 537.822 kb on - strand, within Psyr_0495 at 537.853 kb on + strand at 537.910 kb on + strand at 537.911 kb on - strand at 537.966 kb on + strand at 537.966 kb on + strand at 537.966 kb on + strand at 537.966 kb on + strand at 537.966 kb on + strand at 537.967 kb on - strand at 537.984 kb on + strand at 537.984 kb on + strand at 537.984 kb on + strand at 537.984 kb on + strand at 537.984 kb on + strand at 537.990 kb on + strand at 537.990 kb on + strand at 537.991 kb on - strand at 537.991 kb on - strand at 537.991 kb on - strand at 537.994 kb on + strand at 537.995 kb on - strand at 537.995 kb on - strand at 537.998 kb on + strand at 537.998 kb on + strand at 537.998 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.125 mg/ml remove 534,898 + +0.1 534,898 + -0.9 534,947 - +0.8 535,115 + Psyr_0493 0.32 +0.1 535,313 + Psyr_0493 0.75 -1.0 535,313 + Psyr_0493 0.75 +0.4 535,313 + Psyr_0493 0.75 -1.0 535,313 + Psyr_0493 0.75 -1.2 535,314 - Psyr_0493 0.75 +0.8 535,314 - Psyr_0493 0.75 -1.1 535,378 + Psyr_0493 0.88 -1.4 535,430 + +0.2 535,430 + -0.9 535,435 - +0.0 535,486 + +0.3 535,486 + +0.4 535,735 + Psyr_0494 0.12 -0.5 535,736 - Psyr_0494 0.12 +0.2 535,736 - Psyr_0494 0.12 -1.4 535,802 + Psyr_0494 0.17 +2.1 535,803 - Psyr_0494 0.17 -0.1 535,814 - Psyr_0494 0.18 -1.3 535,921 + Psyr_0494 0.25 -0.7 535,922 - Psyr_0494 0.25 -0.7 535,922 - Psyr_0494 0.25 +0.3 536,026 + Psyr_0494 0.32 +0.1 536,027 - Psyr_0494 0.32 -0.2 536,039 - Psyr_0494 0.33 +1.0 536,240 + Psyr_0494 0.47 -1.2 536,444 - Psyr_0494 0.61 +0.1 536,444 - Psyr_0494 0.61 -0.8 536,444 - Psyr_0494 0.61 +1.8 536,452 + Psyr_0494 0.61 -0.1 536,452 + Psyr_0494 0.61 -0.2 536,716 + Psyr_0494 0.79 +0.2 536,717 - Psyr_0494 0.79 -0.7 536,764 + Psyr_0494 0.83 -0.3 536,764 + Psyr_0494 0.83 +0.5 536,765 - Psyr_0494 0.83 -1.1 536,765 - Psyr_0494 0.83 -0.8 536,765 - Psyr_0494 0.83 -1.5 536,788 + Psyr_0494 0.84 +0.6 536,788 + Psyr_0494 0.84 -0.1 536,788 + Psyr_0494 0.84 +1.5 536,914 - -0.5 537,128 - +0.5 537,292 + Psyr_0495 0.18 +0.1 537,293 - Psyr_0495 0.18 +0.3 537,293 - Psyr_0495 0.18 -1.5 537,364 + Psyr_0495 0.28 +0.9 537,365 - Psyr_0495 0.28 -0.2 537,566 - Psyr_0495 0.55 -0.6 537,566 - Psyr_0495 0.55 +0.8 537,587 + Psyr_0495 0.57 +1.0 537,651 + Psyr_0495 0.66 +0.0 537,651 + Psyr_0495 0.66 -0.8 537,674 + Psyr_0495 0.69 -0.1 537,674 + Psyr_0495 0.69 +0.4 537,804 - Psyr_0495 0.86 +1.1 537,804 - Psyr_0495 0.86 -0.1 537,804 - Psyr_0495 0.86 +0.5 537,804 - Psyr_0495 0.86 +0.2 537,822 - Psyr_0495 0.89 +1.2 537,853 + -0.4 537,910 + +0.1 537,911 - -0.5 537,966 + -0.4 537,966 + -0.3 537,966 + +0.6 537,966 + -0.2 537,966 + +0.4 537,967 - +0.8 537,984 + -0.4 537,984 + -0.2 537,984 + +0.1 537,984 + -0.5 537,984 + +0.4 537,990 + -0.4 537,990 + -0.8 537,991 - +1.0 537,991 - -1.1 537,991 - +1.3 537,994 + -0.6 537,995 - -0.3 537,995 - +0.2 537,998 + +0.8 537,998 + +0.4 537,998 + -0.7
Or see this region's nucleotide sequence