Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF860 and GFF861 are separated by 295 nucleotides GFF861 and GFF862 are separated by 111 nucleotides GFF862 and GFF863 are separated by 102 nucleotides
GFF860 - FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8), at 37,281 to 37,901
GFF860
GFF861 - D-serine/D-alanine/glycine transporter, at 38,197 to 39,606
GFF861
GFF862 - Nitric oxide-dependent regulator DnrN or NorA, at 39,718 to 40,380
GFF862
GFF863 - Putative membrane protein, at 40,483 to 41,448
GFF863
Position (kb)
38
39
40 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 37.280 kb on - strand at 37.323 kb on + strand at 37.425 kb on - strand, within GFF860 at 37.592 kb on - strand, within GFF860 at 37.648 kb on + strand, within GFF860 at 37.660 kb on - strand, within GFF860 at 37.660 kb on - strand, within GFF860 at 37.802 kb on + strand, within GFF860 at 37.930 kb on - strand at 38.036 kb on - strand at 38.080 kb on + strand at 38.102 kb on + strand at 38.107 kb on - strand at 38.125 kb on + strand at 38.139 kb on + strand at 38.144 kb on + strand at 38.144 kb on + strand at 38.145 kb on - strand at 38.145 kb on - strand at 38.183 kb on - strand at 38.201 kb on + strand at 38.265 kb on + strand at 38.277 kb on + strand at 38.279 kb on + strand at 38.280 kb on - strand at 38.286 kb on + strand at 38.334 kb on + strand at 38.376 kb on + strand, within GFF861 at 38.376 kb on + strand, within GFF861 at 38.541 kb on + strand, within GFF861 at 38.637 kb on - strand, within GFF861 at 38.637 kb on - strand, within GFF861 at 38.896 kb on - strand, within GFF861 at 38.974 kb on + strand, within GFF861 at 38.997 kb on + strand, within GFF861 at 39.205 kb on - strand, within GFF861 at 39.210 kb on - strand, within GFF861 at 39.252 kb on + strand, within GFF861 at 39.252 kb on + strand, within GFF861 at 39.253 kb on - strand, within GFF861 at 39.276 kb on + strand, within GFF861 at 39.345 kb on + strand, within GFF861 at 39.345 kb on + strand, within GFF861 at 39.386 kb on - strand, within GFF861 at 39.424 kb on + strand, within GFF861 at 39.427 kb on - strand, within GFF861 at 39.488 kb on + strand at 39.489 kb on - strand at 39.492 kb on - strand at 39.575 kb on + strand at 39.580 kb on - strand at 39.643 kb on - strand at 39.649 kb on + strand at 39.809 kb on + strand, within GFF862 at 39.809 kb on + strand, within GFF862 at 39.810 kb on - strand, within GFF862 at 39.851 kb on - strand, within GFF862 at 39.993 kb on + strand, within GFF862 at 39.994 kb on - strand, within GFF862 at 40.155 kb on + strand, within GFF862 at 40.156 kb on - strand, within GFF862 at 40.228 kb on + strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.303 kb on - strand, within GFF862 at 40.461 kb on + strand at 40.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 37,280 - -0.1 37,323 + -3.0 37,425 - GFF860 0.23 -1.2 37,592 - GFF860 0.50 -0.1 37,648 + GFF860 0.59 +0.2 37,660 - GFF860 0.61 -0.1 37,660 - GFF860 0.61 -1.2 37,802 + GFF860 0.84 -0.2 37,930 - -0.4 38,036 - -0.1 38,080 + -0.1 38,102 + +0.3 38,107 - +0.1 38,125 + +0.0 38,139 + -0.1 38,144 + -0.0 38,144 + +0.3 38,145 - +0.3 38,145 - +0.3 38,183 - +0.1 38,201 + +0.4 38,265 + +0.6 38,277 + +0.1 38,279 + +0.1 38,280 - +0.7 38,286 + +0.2 38,334 + -0.5 38,376 + GFF861 0.13 +0.1 38,376 + GFF861 0.13 -0.2 38,541 + GFF861 0.24 -0.1 38,637 - GFF861 0.31 +0.5 38,637 - GFF861 0.31 +1.1 38,896 - GFF861 0.50 +1.1 38,974 + GFF861 0.55 -0.3 38,997 + GFF861 0.57 -0.3 39,205 - GFF861 0.71 +0.9 39,210 - GFF861 0.72 -0.2 39,252 + GFF861 0.75 +0.4 39,252 + GFF861 0.75 +0.6 39,253 - GFF861 0.75 -0.6 39,276 + GFF861 0.77 +0.0 39,345 + GFF861 0.81 +0.6 39,345 + GFF861 0.81 +0.2 39,386 - GFF861 0.84 -1.0 39,424 + GFF861 0.87 -0.6 39,427 - GFF861 0.87 -0.5 39,488 + +0.2 39,489 - -0.7 39,492 - -0.4 39,575 + -0.5 39,580 - +0.3 39,643 - +0.2 39,649 + -0.5 39,809 + GFF862 0.14 -0.0 39,809 + GFF862 0.14 +1.0 39,810 - GFF862 0.14 -0.5 39,851 - GFF862 0.20 +0.1 39,993 + GFF862 0.41 +0.2 39,994 - GFF862 0.42 +0.3 40,155 + GFF862 0.66 -0.1 40,156 - GFF862 0.66 -0.0 40,228 + GFF862 0.77 +0.1 40,229 - GFF862 0.77 -0.3 40,229 - GFF862 0.77 -0.5 40,229 - GFF862 0.77 +0.2 40,229 - GFF862 0.77 -0.4 40,303 - GFF862 0.88 -0.6 40,461 + +0.7 40,462 - +0.1
Or see this region's nucleotide sequence