Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF501 and GFF502 are separated by 350 nucleotides GFF502 and GFF503 are separated by 72 nucleotides
GFF501 - Inner membrane metabolite transport protein YhjE, at 228,704 to 230,026
GFF501
GFF502 - Inner membrane protein YhjD, at 230,377 to 231,405
GFF502
GFF503 - LysR family transcriptional regulator YhjC, at 231,478 to 232,380
GFF503
Position (kb) 230
231
232 Strain fitness (log2 ratio) -2
-1
0
1 at 229.836 kb on - strand, within GFF501 at 229.836 kb on - strand, within GFF501 at 229.873 kb on + strand, within GFF501 at 229.906 kb on - strand at 229.910 kb on - strand at 229.912 kb on - strand at 229.954 kb on + strand at 230.283 kb on + strand at 230.378 kb on + strand at 230.417 kb on + strand at 230.547 kb on - strand, within GFF502 at 230.812 kb on + strand, within GFF502 at 230.847 kb on - strand, within GFF502 at 230.988 kb on + strand, within GFF502 at 231.009 kb on + strand, within GFF502 at 231.039 kb on + strand, within GFF502 at 231.061 kb on + strand, within GFF502 at 231.061 kb on + strand, within GFF502 at 231.168 kb on - strand, within GFF502 at 231.436 kb on + strand at 231.436 kb on + strand at 231.436 kb on + strand at 231.437 kb on - strand at 231.444 kb on + strand at 231.446 kb on + strand at 231.446 kb on + strand at 231.514 kb on + strand at 231.520 kb on - strand at 231.670 kb on + strand, within GFF503 at 231.804 kb on - strand, within GFF503 at 231.866 kb on + strand, within GFF503 at 232.000 kb on - strand, within GFF503 at 232.000 kb on - strand, within GFF503 at 232.000 kb on - strand, within GFF503 at 232.086 kb on + strand, within GFF503 at 232.087 kb on - strand, within GFF503 at 232.087 kb on - strand, within GFF503 at 232.091 kb on - strand, within GFF503 at 232.203 kb on + strand, within GFF503
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 229,836 - GFF501 0.86 -0.2 229,836 - GFF501 0.86 -0.9 229,873 + GFF501 0.88 -0.1 229,906 - +0.1 229,910 - +0.1 229,912 - +0.2 229,954 + -0.0 230,283 + -0.8 230,378 + +1.6 230,417 + +0.2 230,547 - GFF502 0.17 -1.2 230,812 + GFF502 0.42 -0.1 230,847 - GFF502 0.46 -0.2 230,988 + GFF502 0.59 +1.2 231,009 + GFF502 0.61 +0.5 231,039 + GFF502 0.64 +0.4 231,061 + GFF502 0.66 -0.0 231,061 + GFF502 0.66 -0.8 231,168 - GFF502 0.77 -0.2 231,436 + -0.1 231,436 + +0.0 231,436 + +0.2 231,437 - +0.4 231,444 + -0.2 231,446 + +0.1 231,446 + -0.0 231,514 + +0.1 231,520 - -0.1 231,670 + GFF503 0.21 -0.6 231,804 - GFF503 0.36 +0.1 231,866 + GFF503 0.43 -0.1 232,000 - GFF503 0.58 -0.1 232,000 - GFF503 0.58 +0.2 232,000 - GFF503 0.58 +0.3 232,086 + GFF503 0.67 +0.3 232,087 - GFF503 0.67 +0.3 232,087 - GFF503 0.67 +0.5 232,091 - GFF503 0.68 -0.3 232,203 + GFF503 0.80 -2.4
Or see this region's nucleotide sequence