Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4511 and GFF4512 are separated by 316 nucleotides GFF4512 and GFF4513 are separated by 380 nucleotides
GFF4511 - LysR-family transcriptional regulator YbeF, at 298,257 to 299,159
GFF4511
GFF4512 - Thiol:disulfide interchange protein DsbG precursor, at 299,476 to 300,282
GFF4512
GFF4513 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 300,663 to 301,226
GFF4513
Position (kb) 299
300
301 Strain fitness (log2 ratio) -1
0
1 at 299.110 kb on - strand at 299.425 kb on + strand at 299.425 kb on + strand at 299.561 kb on + strand, within GFF4512 at 299.599 kb on + strand, within GFF4512 at 299.867 kb on + strand, within GFF4512 at 299.867 kb on + strand, within GFF4512 at 299.868 kb on - strand, within GFF4512 at 299.868 kb on - strand, within GFF4512 at 299.955 kb on - strand, within GFF4512 at 300.005 kb on + strand, within GFF4512 at 300.006 kb on - strand, within GFF4512 at 300.009 kb on + strand, within GFF4512 at 300.009 kb on + strand, within GFF4512 at 300.009 kb on + strand, within GFF4512 at 300.011 kb on + strand, within GFF4512 at 300.048 kb on - strand, within GFF4512 at 300.196 kb on - strand, within GFF4512 at 300.217 kb on - strand at 300.282 kb on + strand at 300.283 kb on - strand at 300.283 kb on - strand at 300.347 kb on + strand at 300.348 kb on - strand at 300.392 kb on + strand at 300.394 kb on + strand at 300.394 kb on + strand at 300.395 kb on - strand at 300.423 kb on - strand at 300.568 kb on - strand at 300.791 kb on + strand, within GFF4513 at 300.834 kb on + strand, within GFF4513 at 300.835 kb on - strand, within GFF4513 at 300.943 kb on - strand, within GFF4513 at 301.156 kb on + strand, within GFF4513
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 299,110 - +0.4 299,425 + -1.0 299,425 + +0.3 299,561 + GFF4512 0.11 +0.4 299,599 + GFF4512 0.15 +1.4 299,867 + GFF4512 0.48 +0.2 299,867 + GFF4512 0.48 -0.1 299,868 - GFF4512 0.49 +0.7 299,868 - GFF4512 0.49 +0.1 299,955 - GFF4512 0.59 +0.4 300,005 + GFF4512 0.66 +0.4 300,006 - GFF4512 0.66 -0.3 300,009 + GFF4512 0.66 +0.1 300,009 + GFF4512 0.66 +0.9 300,009 + GFF4512 0.66 -0.2 300,011 + GFF4512 0.66 -1.2 300,048 - GFF4512 0.71 +1.0 300,196 - GFF4512 0.89 -0.2 300,217 - -0.2 300,282 + +0.2 300,283 - +0.1 300,283 - +0.4 300,347 + -0.0 300,348 - +0.0 300,392 + +0.0 300,394 + +0.2 300,394 + +0.1 300,395 - +0.5 300,423 - -0.1 300,568 - -0.1 300,791 + GFF4513 0.23 +0.6 300,834 + GFF4513 0.30 +0.2 300,835 - GFF4513 0.30 +0.2 300,943 - GFF4513 0.50 -1.1 301,156 + GFF4513 0.87 +1.0
Or see this region's nucleotide sequence