Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4345 and GFF4346 are separated by 10 nucleotides GFF4346 and GFF4347 are separated by 273 nucleotides GFF4347 and GFF4348 are separated by 83 nucleotides
GFF4345 - Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-), at 125,401 to 127,392
GFF4345
GFF4346 - Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-), at 127,403 to 128,332
GFF4346
GFF4347 - hypothetical protein, at 128,606 to 128,728
GFF4347
GFF4348 - AmpG permease, at 128,812 to 130,287
GFF4348
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 126.569 kb on + strand, within GFF4345 at 126.569 kb on + strand, within GFF4345 at 126.569 kb on + strand, within GFF4345 at 126.570 kb on - strand, within GFF4345 at 126.620 kb on + strand, within GFF4345 at 126.792 kb on + strand, within GFF4345 at 126.843 kb on + strand, within GFF4345 at 126.851 kb on + strand, within GFF4345 at 126.851 kb on + strand, within GFF4345 at 126.852 kb on - strand, within GFF4345 at 126.852 kb on - strand, within GFF4345 at 127.210 kb on - strand at 127.211 kb on + strand at 127.211 kb on + strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.212 kb on - strand at 127.231 kb on - strand at 127.266 kb on - strand at 127.276 kb on - strand at 127.348 kb on - strand at 127.394 kb on + strand at 127.395 kb on + strand at 127.395 kb on + strand at 127.396 kb on - strand at 127.396 kb on - strand at 127.444 kb on + strand at 127.445 kb on - strand at 127.445 kb on - strand at 127.529 kb on - strand, within GFF4346 at 127.529 kb on - strand, within GFF4346 at 127.566 kb on + strand, within GFF4346 at 127.567 kb on - strand, within GFF4346 at 127.595 kb on - strand, within GFF4346 at 127.618 kb on + strand, within GFF4346 at 127.693 kb on + strand, within GFF4346 at 127.752 kb on + strand, within GFF4346 at 127.752 kb on + strand, within GFF4346 at 127.810 kb on - strand, within GFF4346 at 127.936 kb on - strand, within GFF4346 at 128.127 kb on + strand, within GFF4346 at 128.127 kb on + strand, within GFF4346 at 128.143 kb on + strand, within GFF4346 at 128.316 kb on - strand at 128.338 kb on + strand at 128.429 kb on + strand at 128.481 kb on + strand at 128.493 kb on + strand at 128.493 kb on + strand at 128.509 kb on + strand at 128.774 kb on + strand at 128.813 kb on + strand at 128.925 kb on + strand at 128.930 kb on + strand at 129.064 kb on - strand, within GFF4348 at 129.064 kb on - strand, within GFF4348 at 129.064 kb on - strand, within GFF4348 at 129.065 kb on + strand, within GFF4348 at 129.066 kb on - strand, within GFF4348 at 129.066 kb on - strand, within GFF4348 at 129.250 kb on - strand, within GFF4348 at 129.250 kb on - strand, within GFF4348 at 129.253 kb on - strand, within GFF4348 at 129.253 kb on - strand, within GFF4348
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 126,569 + GFF4345 0.59 +0.5 126,569 + GFF4345 0.59 -0.2 126,569 + GFF4345 0.59 -0.5 126,570 - GFF4345 0.59 +0.1 126,620 + GFF4345 0.61 +0.2 126,792 + GFF4345 0.70 +0.6 126,843 + GFF4345 0.72 +0.4 126,851 + GFF4345 0.73 +0.3 126,851 + GFF4345 0.73 +0.5 126,852 - GFF4345 0.73 +0.5 126,852 - GFF4345 0.73 +0.7 127,210 - +0.7 127,211 + -0.1 127,211 + +0.5 127,212 - +0.6 127,212 - +0.9 127,212 - +1.4 127,212 - +0.7 127,212 - +1.3 127,231 - +0.5 127,266 - +1.2 127,276 - -0.9 127,348 - +0.9 127,394 + +0.3 127,395 + +0.3 127,395 + +0.5 127,396 - +0.8 127,396 - +0.4 127,444 + +1.0 127,445 - +0.3 127,445 - +0.5 127,529 - GFF4346 0.14 +0.4 127,529 - GFF4346 0.14 +0.4 127,566 + GFF4346 0.18 +0.4 127,567 - GFF4346 0.18 +1.1 127,595 - GFF4346 0.21 +0.7 127,618 + GFF4346 0.23 +1.0 127,693 + GFF4346 0.31 +0.5 127,752 + GFF4346 0.38 +0.1 127,752 + GFF4346 0.38 +0.1 127,810 - GFF4346 0.44 -0.5 127,936 - GFF4346 0.57 +0.8 128,127 + GFF4346 0.78 +0.2 128,127 + GFF4346 0.78 -0.6 128,143 + GFF4346 0.80 +2.6 128,316 - -2.6 128,338 + +0.2 128,429 + +0.3 128,481 + +0.3 128,493 + +0.6 128,493 + -0.9 128,509 + -1.0 128,774 + +0.7 128,813 + +0.1 128,925 + -1.7 128,930 + -0.9 129,064 - GFF4348 0.17 -0.0 129,064 - GFF4348 0.17 +0.4 129,064 - GFF4348 0.17 -0.1 129,065 + GFF4348 0.17 +0.1 129,066 - GFF4348 0.17 +0.0 129,066 - GFF4348 0.17 +0.8 129,250 - GFF4348 0.30 -2.4 129,250 - GFF4348 0.30 -0.7 129,253 - GFF4348 0.30 +0.2 129,253 - GFF4348 0.30 +0.2
Or see this region's nucleotide sequence