Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4247 and GFF4248 are separated by 276 nucleotides GFF4248 and GFF4249 are separated by 11 nucleotides GFF4249 and GFF4250 are separated by 77 nucleotides
GFF4247 - RND efflux system, membrane fusion protein CmeA, at 27,269 to 28,495
GFF4247
GFF4248 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 28,772 to 31,060
GFF4248
GFF4249 - Cu(I)-responsive transcriptional regulator, at 31,072 to 31,536
GFF4249
GFF4250 - Putative copper chaperone, at 31,614 to 31,808
GFF4250
Position (kb) 28
29
30
31
32 Strain fitness (log2 ratio) -2
-1
0
1
2 at 27.828 kb on + strand, within GFF4247 at 27.828 kb on + strand, within GFF4247 at 27.828 kb on + strand, within GFF4247 at 27.828 kb on + strand, within GFF4247 at 27.829 kb on - strand, within GFF4247 at 27.851 kb on - strand, within GFF4247 at 27.914 kb on + strand, within GFF4247 at 27.950 kb on - strand, within GFF4247 at 28.125 kb on - strand, within GFF4247 at 28.139 kb on + strand, within GFF4247 at 28.140 kb on - strand, within GFF4247 at 28.330 kb on + strand, within GFF4247 at 28.346 kb on - strand, within GFF4247 at 28.413 kb on - strand at 28.432 kb on - strand at 28.475 kb on - strand at 28.489 kb on + strand at 28.489 kb on + strand at 28.490 kb on - strand at 28.636 kb on + strand at 28.751 kb on - strand at 28.809 kb on + strand at 28.810 kb on - strand at 28.810 kb on - strand at 28.813 kb on + strand at 28.822 kb on + strand at 28.822 kb on + strand at 28.979 kb on - strand at 28.992 kb on + strand at 29.125 kb on + strand, within GFF4248 at 29.135 kb on - strand, within GFF4248 at 29.376 kb on - strand, within GFF4248 at 29.378 kb on + strand, within GFF4248 at 29.465 kb on - strand, within GFF4248 at 29.992 kb on + strand, within GFF4248 at 29.992 kb on + strand, within GFF4248 at 29.993 kb on - strand, within GFF4248 at 29.996 kb on - strand, within GFF4248 at 30.302 kb on + strand, within GFF4248 at 30.302 kb on + strand, within GFF4248 at 30.303 kb on - strand, within GFF4248 at 30.303 kb on - strand, within GFF4248 at 30.347 kb on + strand, within GFF4248 at 30.349 kb on + strand, within GFF4248 at 30.350 kb on - strand, within GFF4248 at 30.429 kb on - strand, within GFF4248 at 30.514 kb on - strand, within GFF4248 at 30.780 kb on + strand, within GFF4248 at 30.781 kb on - strand, within GFF4248 at 30.784 kb on - strand, within GFF4248 at 30.818 kb on - strand, within GFF4248 at 30.915 kb on + strand at 30.936 kb on + strand at 31.093 kb on - strand at 31.204 kb on - strand, within GFF4249 at 31.268 kb on + strand, within GFF4249 at 31.268 kb on + strand, within GFF4249 at 31.534 kb on + strand at 31.534 kb on + strand at 31.548 kb on - strand at 31.692 kb on - strand, within GFF4250 at 31.806 kb on + strand at 31.807 kb on - strand at 31.976 kb on - strand at 31.996 kb on + strand at 32.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 27,828 + GFF4247 0.46 +0.2 27,828 + GFF4247 0.46 -0.1 27,828 + GFF4247 0.46 +0.0 27,828 + GFF4247 0.46 -0.2 27,829 - GFF4247 0.46 -0.1 27,851 - GFF4247 0.47 -0.7 27,914 + GFF4247 0.53 +0.6 27,950 - GFF4247 0.56 +0.6 28,125 - GFF4247 0.70 +1.2 28,139 + GFF4247 0.71 -0.0 28,140 - GFF4247 0.71 -0.1 28,330 + GFF4247 0.86 -0.5 28,346 - GFF4247 0.88 +1.0 28,413 - +0.0 28,432 - -0.1 28,475 - -0.3 28,489 + -0.0 28,489 + -0.8 28,490 - -0.3 28,636 + -0.5 28,751 - -0.1 28,809 + -0.6 28,810 - -0.2 28,810 - -0.2 28,813 + -0.3 28,822 + +0.2 28,822 + +0.1 28,979 - +0.1 28,992 + +0.5 29,125 + GFF4248 0.15 -0.1 29,135 - GFF4248 0.16 -0.2 29,376 - GFF4248 0.26 +2.3 29,378 + GFF4248 0.26 -0.4 29,465 - GFF4248 0.30 +1.0 29,992 + GFF4248 0.53 -0.7 29,992 + GFF4248 0.53 -0.7 29,993 - GFF4248 0.53 -0.5 29,996 - GFF4248 0.53 -0.2 30,302 + GFF4248 0.67 +0.3 30,302 + GFF4248 0.67 +1.1 30,303 - GFF4248 0.67 -0.3 30,303 - GFF4248 0.67 +0.5 30,347 + GFF4248 0.69 -0.5 30,349 + GFF4248 0.69 +0.1 30,350 - GFF4248 0.69 -0.2 30,429 - GFF4248 0.72 -1.4 30,514 - GFF4248 0.76 -0.8 30,780 + GFF4248 0.88 -0.7 30,781 - GFF4248 0.88 +0.3 30,784 - GFF4248 0.88 +0.2 30,818 - GFF4248 0.89 -0.5 30,915 + -0.2 30,936 + -0.1 31,093 - +0.2 31,204 - GFF4249 0.28 -0.8 31,268 + GFF4249 0.42 -0.5 31,268 + GFF4249 0.42 +0.0 31,534 + +0.2 31,534 + -0.4 31,548 - +0.6 31,692 - GFF4250 0.40 +0.5 31,806 + -0.3 31,807 - -1.8 31,976 - +0.4 31,996 + -0.0 32,012 - +0.2
Or see this region's nucleotide sequence