Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3375 and GFF3376 are separated by 155 nucleotides GFF3376 and GFF3377 are separated by 121 nucleotides GFF3377 and GFF3378 are separated by 123 nucleotides GFF3378 and GFF3379 are separated by 2 nucleotides
GFF3375 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 141,275 to 142,009
GFF3375
GFF3376 - Acyl carrier protein, at 142,165 to 142,401
GFF3376
GFF3377 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 142,523 to 143,728
GFF3377
GFF3378 - Aminodeoxychorismate lyase (EC 4.1.3.38), at 143,852 to 144,661
GFF3378
GFF3379 - FIG004453: protein YceG like, at 144,664 to 145,686
GFF3379
Position (kb) 142
143
144 Strain fitness (log2 ratio) -2
-1
0
1 at 142.033 kb on + strand at 142.033 kb on + strand at 142.456 kb on + strand at 142.492 kb on + strand at 142.549 kb on - strand at 142.644 kb on + strand, within GFF3377 at 142.657 kb on + strand, within GFF3377 at 142.931 kb on - strand, within GFF3377 at 142.931 kb on - strand, within GFF3377 at 142.937 kb on + strand, within GFF3377 at 142.937 kb on + strand, within GFF3377 at 142.938 kb on - strand, within GFF3377 at 143.085 kb on + strand, within GFF3377 at 143.288 kb on + strand, within GFF3377 at 143.288 kb on + strand, within GFF3377 at 143.294 kb on - strand, within GFF3377 at 143.533 kb on - strand, within GFF3377 at 143.533 kb on - strand, within GFF3377 at 143.539 kb on + strand, within GFF3377 at 143.698 kb on + strand at 143.726 kb on + strand at 143.790 kb on - strand at 143.892 kb on + strand at 144.507 kb on + strand, within GFF3378 at 144.575 kb on - strand, within GFF3378
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 142,033 + -0.3 142,033 + -0.7 142,456 + +1.0 142,492 + +0.3 142,549 - +0.4 142,644 + GFF3377 0.10 -0.8 142,657 + GFF3377 0.11 +0.1 142,931 - GFF3377 0.34 -0.4 142,931 - GFF3377 0.34 -1.0 142,937 + GFF3377 0.34 -0.8 142,937 + GFF3377 0.34 -2.4 142,938 - GFF3377 0.34 -1.2 143,085 + GFF3377 0.47 -0.2 143,288 + GFF3377 0.63 +0.6 143,288 + GFF3377 0.63 -1.0 143,294 - GFF3377 0.64 -0.2 143,533 - GFF3377 0.84 -0.6 143,533 - GFF3377 0.84 +0.8 143,539 + GFF3377 0.84 +0.6 143,698 + -1.4 143,726 + +0.3 143,790 - +1.0 143,892 + -0.0 144,507 + GFF3378 0.81 -0.3 144,575 - GFF3378 0.89 -0.1
Or see this region's nucleotide sequence