Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2807 and GFF2808 are separated by 192 nucleotides GFF2808 and GFF2809 are separated by 108 nucleotides GFF2809 and GFF2810 are separated by 71 nucleotides GFF2810 and GFF2811 are separated by 12 nucleotides GFF2811 and GFF2812 are separated by 62 nucleotides
GFF2807 - Na+/H+ antiporter NhaB, at 332,097 to 333,641
GFF2807
GFF2808 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 333,834 to 334,313
GFF2808
GFF2809 - FIG074102: hypothetical protein, at 334,422 to 334,763
GFF2809
GFF2810 - Gns protein, at 334,835 to 335,008
GFF2810
GFF2811 - hypothetical protein, at 335,021 to 335,137
GFF2811
GFF2812 - hypothetical protein, at 335,200 to 335,337
GFF2812
Position (kb) 333
334
335 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 332.848 kb on + strand, within GFF2807 at 332.995 kb on + strand, within GFF2807 at 333.263 kb on - strand, within GFF2807 at 333.274 kb on - strand, within GFF2807 at 333.274 kb on - strand, within GFF2807 at 333.330 kb on + strand, within GFF2807 at 333.516 kb on - strand at 333.542 kb on + strand at 333.543 kb on - strand at 333.565 kb on + strand at 333.698 kb on + strand at 333.698 kb on + strand at 333.699 kb on - strand at 333.829 kb on + strand at 333.910 kb on + strand, within GFF2808 at 333.910 kb on + strand, within GFF2808 at 333.910 kb on + strand, within GFF2808 at 333.914 kb on + strand, within GFF2808 at 333.914 kb on + strand, within GFF2808 at 333.918 kb on + strand, within GFF2808 at 333.918 kb on + strand, within GFF2808 at 334.017 kb on + strand, within GFF2808 at 334.036 kb on + strand, within GFF2808 at 334.055 kb on + strand, within GFF2808 at 334.267 kb on + strand at 334.421 kb on + strand at 334.422 kb on - strand at 334.432 kb on + strand at 334.432 kb on + strand at 334.433 kb on - strand at 334.467 kb on - strand, within GFF2809 at 334.572 kb on + strand, within GFF2809 at 334.660 kb on - strand, within GFF2809 at 334.673 kb on - strand, within GFF2809 at 334.902 kb on - strand, within GFF2810 at 335.135 kb on + strand at 335.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 332,848 + GFF2807 0.49 -0.1 332,995 + GFF2807 0.58 -0.2 333,263 - GFF2807 0.75 -0.5 333,274 - GFF2807 0.76 +0.4 333,274 - GFF2807 0.76 +0.1 333,330 + GFF2807 0.80 +0.5 333,516 - +0.3 333,542 + -0.1 333,543 - -0.8 333,565 + +0.1 333,698 + -0.1 333,698 + +0.2 333,699 - +0.2 333,829 + -5.2 333,910 + GFF2808 0.16 -3.6 333,910 + GFF2808 0.16 -4.3 333,910 + GFF2808 0.16 -4.4 333,914 + GFF2808 0.17 -3.0 333,914 + GFF2808 0.17 -4.5 333,918 + GFF2808 0.17 -5.3 333,918 + GFF2808 0.17 -1.9 334,017 + GFF2808 0.38 -3.7 334,036 + GFF2808 0.42 -3.2 334,055 + GFF2808 0.46 -5.3 334,267 + +0.8 334,421 + +0.7 334,422 - +0.3 334,432 + +0.2 334,432 + +0.0 334,433 - -0.8 334,467 - GFF2809 0.13 -0.5 334,572 + GFF2809 0.44 -0.3 334,660 - GFF2809 0.70 -0.9 334,673 - GFF2809 0.73 +0.4 334,902 - GFF2810 0.39 +1.4 335,135 + +0.3 335,206 - -0.4
Or see this region's nucleotide sequence