Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF253 and GFF254 are separated by 248 nucleotides GFF254 and GFF255 are separated by 63 nucleotides GFF255 and GFF256 are separated by 199 nucleotides
GFF253 - Transcriptional activator MetR, at 27,394 to 28,347
GFF253
GFF254 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 28,596 to 30,860
GFF254
GFF255 - hypothetical protein, at 30,924 to 31,052
GFF255
GFF256 - Putative arylsulfatase regulatory protein, at 31,252 to 32,547
GFF256
Position (kb)
28
29
30
31 Strain fitness (log2 ratio)
-1
0
1 at 27.811 kb on - strand, within GFF253 at 27.885 kb on - strand, within GFF253 at 28.048 kb on - strand, within GFF253 at 28.053 kb on + strand, within GFF253 at 28.053 kb on + strand, within GFF253 at 28.053 kb on + strand, within GFF253 at 28.065 kb on + strand, within GFF253 at 28.336 kb on - strand at 28.390 kb on + strand at 28.390 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.392 kb on + strand at 28.397 kb on + strand at 28.403 kb on + strand at 28.447 kb on - strand at 28.494 kb on - strand at 28.494 kb on - strand at 28.671 kb on + strand at 28.772 kb on - strand at 28.956 kb on - strand, within GFF254 at 28.958 kb on + strand, within GFF254 at 28.958 kb on + strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.959 kb on - strand, within GFF254 at 28.962 kb on + strand, within GFF254 at 28.963 kb on - strand, within GFF254 at 28.963 kb on - strand, within GFF254 at 28.968 kb on + strand, within GFF254 at 28.969 kb on + strand, within GFF254 at 28.969 kb on - strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.972 kb on + strand, within GFF254 at 28.973 kb on - strand, within GFF254 at 28.973 kb on - strand, within GFF254 at 28.973 kb on - strand, within GFF254 at 29.074 kb on + strand, within GFF254 at 29.074 kb on + strand, within GFF254 at 29.094 kb on + strand, within GFF254 at 29.094 kb on + strand, within GFF254 at 29.094 kb on + strand, within GFF254 at 29.095 kb on - strand, within GFF254 at 29.095 kb on - strand, within GFF254 at 29.095 kb on - strand, within GFF254 at 29.166 kb on + strand, within GFF254 at 29.176 kb on + strand, within GFF254 at 29.176 kb on + strand, within GFF254 at 29.201 kb on + strand, within GFF254 at 29.201 kb on + strand, within GFF254 at 29.322 kb on + strand, within GFF254 at 29.354 kb on + strand, within GFF254 at 29.380 kb on + strand, within GFF254 at 29.380 kb on + strand, within GFF254 at 29.414 kb on - strand, within GFF254 at 29.759 kb on + strand, within GFF254 at 29.759 kb on + strand, within GFF254 at 29.987 kb on + strand, within GFF254 at 29.987 kb on + strand, within GFF254 at 30.037 kb on + strand, within GFF254 at 30.129 kb on + strand, within GFF254 at 30.130 kb on - strand, within GFF254 at 30.210 kb on + strand, within GFF254 at 30.211 kb on - strand, within GFF254 at 30.381 kb on - strand, within GFF254 at 30.525 kb on + strand, within GFF254 at 30.525 kb on + strand, within GFF254 at 30.738 kb on - strand at 30.738 kb on - strand at 30.858 kb on + strand at 30.859 kb on - strand at 31.031 kb on - strand, within GFF255 at 31.099 kb on + strand at 31.099 kb on + strand at 31.175 kb on + strand at 31.175 kb on + strand at 31.176 kb on - strand at 31.275 kb on - strand at 31.318 kb on + strand at 31.318 kb on + strand at 31.327 kb on + strand at 31.328 kb on - strand at 31.328 kb on - strand at 31.401 kb on + strand, within GFF256 at 31.401 kb on + strand, within GFF256 at 31.403 kb on + strand, within GFF256 at 31.413 kb on + strand, within GFF256 at 31.509 kb on - strand, within GFF256 at 31.646 kb on + strand, within GFF256 at 31.646 kb on + strand, within GFF256 at 31.666 kb on - strand, within GFF256 at 31.741 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.824 kb on + strand, within GFF256 at 31.845 kb on - strand, within GFF256
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 27,811 - GFF253 0.44 +0.5 27,885 - GFF253 0.51 +0.2 28,048 - GFF253 0.69 +0.9 28,053 + GFF253 0.69 +0.2 28,053 + GFF253 0.69 +0.5 28,053 + GFF253 0.69 +0.3 28,065 + GFF253 0.70 -1.1 28,336 - +0.3 28,390 + +0.4 28,390 + +0.3 28,392 + -0.5 28,392 + -0.1 28,392 + +0.6 28,392 + +0.6 28,392 + +0.7 28,392 + +0.6 28,392 + -0.4 28,397 + -0.0 28,403 + +0.8 28,447 - +0.1 28,494 - +0.7 28,494 - +0.4 28,671 + -0.2 28,772 - -0.3 28,956 - GFF254 0.16 -0.2 28,958 + GFF254 0.16 -0.5 28,958 + GFF254 0.16 +0.1 28,959 - GFF254 0.16 -0.2 28,959 - GFF254 0.16 -0.9 28,959 - GFF254 0.16 +0.5 28,959 - GFF254 0.16 +1.2 28,959 - GFF254 0.16 -0.0 28,962 + GFF254 0.16 -0.5 28,963 - GFF254 0.16 +0.8 28,963 - GFF254 0.16 -0.7 28,968 + GFF254 0.16 -0.2 28,969 + GFF254 0.16 +0.9 28,969 - GFF254 0.16 +0.4 28,972 + GFF254 0.17 +0.1 28,972 + GFF254 0.17 +0.1 28,972 + GFF254 0.17 +0.1 28,972 + GFF254 0.17 +0.8 28,972 + GFF254 0.17 -0.5 28,972 + GFF254 0.17 -0.2 28,973 - GFF254 0.17 +0.7 28,973 - GFF254 0.17 +0.7 28,973 - GFF254 0.17 -0.2 29,074 + GFF254 0.21 -0.1 29,074 + GFF254 0.21 +1.5 29,094 + GFF254 0.22 +0.1 29,094 + GFF254 0.22 +0.0 29,094 + GFF254 0.22 +0.6 29,095 - GFF254 0.22 +0.5 29,095 - GFF254 0.22 +0.3 29,095 - GFF254 0.22 -0.0 29,166 + GFF254 0.25 +0.0 29,176 + GFF254 0.26 -0.1 29,176 + GFF254 0.26 -0.8 29,201 + GFF254 0.27 -0.4 29,201 + GFF254 0.27 -0.1 29,322 + GFF254 0.32 -0.3 29,354 + GFF254 0.33 -0.8 29,380 + GFF254 0.35 +0.1 29,380 + GFF254 0.35 +0.5 29,414 - GFF254 0.36 +0.2 29,759 + GFF254 0.51 +0.4 29,759 + GFF254 0.51 +0.3 29,987 + GFF254 0.61 +0.4 29,987 + GFF254 0.61 -0.1 30,037 + GFF254 0.64 +0.1 30,129 + GFF254 0.68 +0.6 30,130 - GFF254 0.68 +0.4 30,210 + GFF254 0.71 -0.7 30,211 - GFF254 0.71 +1.4 30,381 - GFF254 0.79 +1.0 30,525 + GFF254 0.85 -0.0 30,525 + GFF254 0.85 +0.1 30,738 - +0.6 30,738 - +0.2 30,858 + +0.2 30,859 - -0.1 31,031 - GFF255 0.83 -0.0 31,099 + +0.2 31,099 + +0.6 31,175 + +0.3 31,175 + -0.2 31,176 - +0.7 31,275 - +0.8 31,318 + +0.0 31,318 + -0.0 31,327 + +0.2 31,328 - +0.4 31,328 - +1.1 31,401 + GFF256 0.11 -0.1 31,401 + GFF256 0.11 -0.4 31,403 + GFF256 0.12 -0.2 31,413 + GFF256 0.12 +0.1 31,509 - GFF256 0.20 +0.3 31,646 + GFF256 0.30 -0.6 31,646 + GFF256 0.30 +0.6 31,666 - GFF256 0.32 +0.3 31,741 - GFF256 0.38 -0.1 31,744 - GFF256 0.38 +0.6 31,744 - GFF256 0.38 +0.3 31,824 + GFF256 0.44 +1.2 31,845 - GFF256 0.46 +0.9
Or see this region's nucleotide sequence