Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2222 and GFF2223 are separated by 375 nucleotides GFF2223 and GFF2224 are separated by 54 nucleotides GFF2224 and GFF2225 overlap by 16 nucleotides
GFF2222 - Glutamine synthetase type I (EC 6.3.1.2), at 14,523 to 15,932
GFF2222
GFF2223 - GTP-binding protein TypA/BipA, at 16,308 to 18,131
GFF2223
GFF2224 - Putative hydrolase, at 18,186 to 18,920
GFF2224
GFF2225 - FIG01046022: hypothetical protein, at 18,905 to 19,783
GFF2225
Position (kb) 16
17
18
19 Strain fitness (log2 ratio) -2
-1
0
1 at 15.446 kb on + strand, within GFF2222 at 15.447 kb on - strand, within GFF2222 at 15.501 kb on - strand, within GFF2222 at 15.632 kb on - strand, within GFF2222 at 15.933 kb on - strand at 15.939 kb on - strand at 15.939 kb on - strand at 15.939 kb on - strand at 16.015 kb on - strand at 16.178 kb on + strand at 16.359 kb on - strand at 16.377 kb on - strand at 16.470 kb on - strand at 16.470 kb on - strand at 16.470 kb on - strand at 16.529 kb on + strand, within GFF2223 at 16.865 kb on - strand, within GFF2223 at 16.956 kb on + strand, within GFF2223 at 17.031 kb on - strand, within GFF2223 at 17.231 kb on + strand, within GFF2223 at 17.618 kb on - strand, within GFF2223 at 17.618 kb on - strand, within GFF2223 at 17.831 kb on + strand, within GFF2223 at 17.911 kb on + strand, within GFF2223 at 18.129 kb on + strand at 18.242 kb on + strand at 18.242 kb on + strand at 18.341 kb on + strand, within GFF2224 at 18.397 kb on + strand, within GFF2224 at 18.398 kb on - strand, within GFF2224 at 18.422 kb on + strand, within GFF2224 at 18.423 kb on - strand, within GFF2224 at 18.423 kb on - strand, within GFF2224 at 18.467 kb on + strand, within GFF2224 at 18.475 kb on - strand, within GFF2224 at 18.481 kb on - strand, within GFF2224 at 18.554 kb on + strand, within GFF2224 at 18.596 kb on + strand, within GFF2224 at 18.657 kb on + strand, within GFF2224 at 18.657 kb on + strand, within GFF2224 at 18.710 kb on + strand, within GFF2224 at 18.713 kb on - strand, within GFF2224 at 18.761 kb on - strand, within GFF2224 at 18.763 kb on + strand, within GFF2224 at 18.906 kb on + strand at 18.991 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 15,446 + GFF2222 0.65 +0.5 15,447 - GFF2222 0.66 -0.6 15,501 - GFF2222 0.69 -0.3 15,632 - GFF2222 0.79 -1.0 15,933 - -1.1 15,939 - -0.6 15,939 - -0.6 15,939 - -0.6 16,015 - -0.2 16,178 + +0.1 16,359 - -1.0 16,377 - -1.4 16,470 - -1.1 16,470 - -1.1 16,470 - -1.1 16,529 + GFF2223 0.12 -1.0 16,865 - GFF2223 0.31 -1.0 16,956 + GFF2223 0.36 -1.5 17,031 - GFF2223 0.40 +1.2 17,231 + GFF2223 0.51 -0.7 17,618 - GFF2223 0.72 -0.6 17,618 - GFF2223 0.72 -2.0 17,831 + GFF2223 0.83 -2.4 17,911 + GFF2223 0.88 -1.5 18,129 + -0.3 18,242 + -1.4 18,242 + -0.6 18,341 + GFF2224 0.21 +0.2 18,397 + GFF2224 0.29 -0.9 18,398 - GFF2224 0.29 +0.3 18,422 + GFF2224 0.32 +0.4 18,423 - GFF2224 0.32 -0.1 18,423 - GFF2224 0.32 +0.2 18,467 + GFF2224 0.38 +0.1 18,475 - GFF2224 0.39 -0.6 18,481 - GFF2224 0.40 +0.0 18,554 + GFF2224 0.50 +0.4 18,596 + GFF2224 0.56 +0.1 18,657 + GFF2224 0.64 -0.1 18,657 + GFF2224 0.64 -0.0 18,710 + GFF2224 0.71 -0.2 18,713 - GFF2224 0.72 -0.5 18,761 - GFF2224 0.78 -0.2 18,763 + GFF2224 0.79 +0.2 18,906 + -1.3 18,991 - -0.5
Or see this region's nucleotide sequence