Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1705 and GFF1706 are separated by 133 nucleotides GFF1706 and GFF1707 are separated by 24 nucleotides GFF1707 and GFF1708 are separated by 5 nucleotides
GFF1705 - Ribokinase (EC 2.7.1.15), at 3,299 to 4,228
GFF1705
GFF1706 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 4,362 to 5,252
GFF1706
GFF1707 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 5,277 to 6,242
GFF1707
GFF1708 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 6,248 to 7,753
GFF1708
Position (kb) 4
5
6 Strain fitness (log2 ratio) -1
0
1 at 3.463 kb on + strand, within GFF1705 at 3.530 kb on + strand, within GFF1705 at 3.563 kb on - strand, within GFF1705 at 3.745 kb on + strand, within GFF1705 at 3.746 kb on - strand, within GFF1705 at 3.746 kb on - strand, within GFF1705 at 3.746 kb on - strand, within GFF1705 at 3.748 kb on + strand, within GFF1705 at 3.748 kb on + strand, within GFF1705 at 3.749 kb on - strand, within GFF1705 at 3.749 kb on - strand, within GFF1705 at 3.959 kb on - strand, within GFF1705 at 3.959 kb on - strand, within GFF1705 at 3.967 kb on + strand, within GFF1705 at 3.968 kb on - strand, within GFF1705 at 3.968 kb on - strand, within GFF1705 at 4.006 kb on + strand, within GFF1705 at 4.013 kb on - strand, within GFF1705 at 4.058 kb on - strand, within GFF1705 at 4.070 kb on - strand, within GFF1705 at 4.070 kb on - strand, within GFF1705 at 4.172 kb on - strand at 4.177 kb on + strand at 4.321 kb on - strand at 4.321 kb on - strand at 4.342 kb on + strand at 4.361 kb on - strand at 4.361 kb on - strand at 4.563 kb on - strand, within GFF1706 at 4.677 kb on - strand, within GFF1706 at 4.715 kb on + strand, within GFF1706 at 4.715 kb on + strand, within GFF1706 at 5.122 kb on - strand, within GFF1706 at 5.141 kb on + strand, within GFF1706 at 5.150 kb on + strand, within GFF1706 at 5.471 kb on + strand, within GFF1707 at 5.471 kb on + strand, within GFF1707 at 5.485 kb on + strand, within GFF1707 at 5.573 kb on - strand, within GFF1707 at 5.579 kb on + strand, within GFF1707 at 5.600 kb on + strand, within GFF1707 at 5.648 kb on + strand, within GFF1707 at 5.651 kb on - strand, within GFF1707 at 5.678 kb on + strand, within GFF1707 at 5.678 kb on + strand, within GFF1707 at 5.679 kb on - strand, within GFF1707 at 5.679 kb on - strand, within GFF1707 at 5.680 kb on + strand, within GFF1707 at 5.723 kb on + strand, within GFF1707 at 5.813 kb on - strand, within GFF1707 at 5.836 kb on - strand, within GFF1707
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 3,463 + GFF1705 0.18 -0.4 3,530 + GFF1705 0.25 +0.5 3,563 - GFF1705 0.28 -0.7 3,745 + GFF1705 0.48 -0.3 3,746 - GFF1705 0.48 -0.7 3,746 - GFF1705 0.48 +0.1 3,746 - GFF1705 0.48 +1.1 3,748 + GFF1705 0.48 +0.1 3,748 + GFF1705 0.48 -1.1 3,749 - GFF1705 0.48 +0.5 3,749 - GFF1705 0.48 +0.6 3,959 - GFF1705 0.71 +0.1 3,959 - GFF1705 0.71 -0.5 3,967 + GFF1705 0.72 +0.9 3,968 - GFF1705 0.72 +0.4 3,968 - GFF1705 0.72 -0.2 4,006 + GFF1705 0.76 +1.0 4,013 - GFF1705 0.77 -0.1 4,058 - GFF1705 0.82 -0.2 4,070 - GFF1705 0.83 +0.5 4,070 - GFF1705 0.83 -0.0 4,172 - +0.1 4,177 + +0.2 4,321 - -0.4 4,321 - +0.7 4,342 + +0.3 4,361 - -0.6 4,361 - +0.7 4,563 - GFF1706 0.23 -0.5 4,677 - GFF1706 0.35 -0.1 4,715 + GFF1706 0.40 +0.1 4,715 + GFF1706 0.40 +0.4 5,122 - GFF1706 0.85 +0.4 5,141 + GFF1706 0.87 -0.0 5,150 + GFF1706 0.88 -0.1 5,471 + GFF1707 0.20 +0.3 5,471 + GFF1707 0.20 -0.3 5,485 + GFF1707 0.22 -0.3 5,573 - GFF1707 0.31 +0.5 5,579 + GFF1707 0.31 +0.1 5,600 + GFF1707 0.33 -0.1 5,648 + GFF1707 0.38 +0.4 5,651 - GFF1707 0.39 -0.3 5,678 + GFF1707 0.42 +0.3 5,678 + GFF1707 0.42 +0.2 5,679 - GFF1707 0.42 +0.2 5,679 - GFF1707 0.42 +0.5 5,680 + GFF1707 0.42 +0.5 5,723 + GFF1707 0.46 +0.7 5,813 - GFF1707 0.55 +1.3 5,836 - GFF1707 0.58 +0.3
Or see this region's nucleotide sequence