Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1405 and GFF1406 are separated by 88 nucleotides GFF1406 and GFF1407 are separated by 163 nucleotides
GFF1405 - Maltose/maltodextrin transport ATP-binding protein MalK (EC 3.6.3.19), at 51,011 to 52,120
GFF1405
GFF1406 - Maltoporin (maltose/maltodextrin high-affinity receptor, phage lambda receptor protein), at 52,209 to 53,567
GFF1406
GFF1407 - Maltose operon periplasmic protein MalM, at 53,731 to 54,648
GFF1407
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1 at 51.250 kb on - strand, within GFF1405 at 51.260 kb on + strand, within GFF1405 at 51.261 kb on - strand, within GFF1405 at 51.337 kb on - strand, within GFF1405 at 51.397 kb on + strand, within GFF1405 at 51.550 kb on + strand, within GFF1405 at 51.550 kb on + strand, within GFF1405 at 51.551 kb on - strand, within GFF1405 at 51.674 kb on + strand, within GFF1405 at 51.675 kb on - strand, within GFF1405 at 51.844 kb on + strand, within GFF1405 at 51.844 kb on + strand, within GFF1405 at 51.845 kb on - strand, within GFF1405 at 51.853 kb on - strand, within GFF1405 at 51.933 kb on + strand, within GFF1405 at 51.989 kb on + strand, within GFF1405 at 51.989 kb on + strand, within GFF1405 at 52.162 kb on + strand at 52.205 kb on + strand at 52.374 kb on - strand, within GFF1406 at 52.374 kb on - strand, within GFF1406 at 52.449 kb on - strand, within GFF1406 at 52.459 kb on - strand, within GFF1406 at 52.480 kb on - strand, within GFF1406 at 52.602 kb on + strand, within GFF1406 at 52.618 kb on + strand, within GFF1406 at 52.619 kb on - strand, within GFF1406 at 52.740 kb on + strand, within GFF1406 at 52.760 kb on + strand, within GFF1406 at 52.764 kb on + strand, within GFF1406 at 52.804 kb on + strand, within GFF1406 at 52.889 kb on + strand, within GFF1406 at 52.890 kb on - strand, within GFF1406 at 52.890 kb on - strand, within GFF1406 at 52.890 kb on - strand, within GFF1406 at 52.890 kb on - strand, within GFF1406 at 52.890 kb on - strand, within GFF1406 at 52.983 kb on + strand, within GFF1406 at 52.983 kb on + strand, within GFF1406 at 52.984 kb on - strand, within GFF1406 at 53.013 kb on - strand, within GFF1406 at 53.117 kb on + strand, within GFF1406 at 53.118 kb on - strand, within GFF1406 at 53.127 kb on - strand, within GFF1406 at 53.160 kb on - strand, within GFF1406 at 53.160 kb on - strand, within GFF1406 at 53.175 kb on + strand, within GFF1406 at 53.175 kb on + strand, within GFF1406 at 53.183 kb on + strand, within GFF1406 at 53.187 kb on + strand, within GFF1406 at 53.187 kb on + strand, within GFF1406 at 53.210 kb on + strand, within GFF1406 at 53.210 kb on + strand, within GFF1406 at 53.242 kb on + strand, within GFF1406 at 53.342 kb on + strand, within GFF1406 at 53.342 kb on + strand, within GFF1406 at 53.405 kb on + strand, within GFF1406 at 53.445 kb on + strand at 53.445 kb on + strand at 53.566 kb on - strand at 53.568 kb on + strand at 53.818 kb on + strand at 53.818 kb on + strand at 53.819 kb on - strand at 53.819 kb on - strand at 53.819 kb on - strand at 53.958 kb on + strand, within GFF1407 at 53.977 kb on + strand, within GFF1407 at 53.978 kb on - strand, within GFF1407 at 54.217 kb on - strand, within GFF1407 at 54.246 kb on + strand, within GFF1407 at 54.246 kb on + strand, within GFF1407 at 54.271 kb on + strand, within GFF1407 at 54.289 kb on - strand, within GFF1407 at 54.516 kb on + strand, within GFF1407
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 51,250 - GFF1405 0.22 +0.7 51,260 + GFF1405 0.22 -0.0 51,261 - GFF1405 0.23 +0.3 51,337 - GFF1405 0.29 -0.0 51,397 + GFF1405 0.35 +0.1 51,550 + GFF1405 0.49 -0.4 51,550 + GFF1405 0.49 +0.3 51,551 - GFF1405 0.49 +0.2 51,674 + GFF1405 0.60 -0.6 51,675 - GFF1405 0.60 -0.1 51,844 + GFF1405 0.75 +0.0 51,844 + GFF1405 0.75 +0.4 51,845 - GFF1405 0.75 -0.1 51,853 - GFF1405 0.76 -0.7 51,933 + GFF1405 0.83 -0.3 51,989 + GFF1405 0.88 +0.2 51,989 + GFF1405 0.88 +0.3 52,162 + +0.3 52,205 + -0.1 52,374 - GFF1406 0.12 -0.5 52,374 - GFF1406 0.12 -0.1 52,449 - GFF1406 0.18 -0.0 52,459 - GFF1406 0.18 +0.2 52,480 - GFF1406 0.20 +0.5 52,602 + GFF1406 0.29 +0.1 52,618 + GFF1406 0.30 -0.5 52,619 - GFF1406 0.30 +0.1 52,740 + GFF1406 0.39 +0.2 52,760 + GFF1406 0.41 +0.3 52,764 + GFF1406 0.41 -0.4 52,804 + GFF1406 0.44 +0.4 52,889 + GFF1406 0.50 +0.1 52,890 - GFF1406 0.50 +0.3 52,890 - GFF1406 0.50 +0.1 52,890 - GFF1406 0.50 -0.1 52,890 - GFF1406 0.50 -0.9 52,890 - GFF1406 0.50 +0.0 52,983 + GFF1406 0.57 -0.0 52,983 + GFF1406 0.57 +0.2 52,984 - GFF1406 0.57 -1.6 53,013 - GFF1406 0.59 -2.0 53,117 + GFF1406 0.67 +0.4 53,118 - GFF1406 0.67 +0.1 53,127 - GFF1406 0.68 -0.6 53,160 - GFF1406 0.70 -0.0 53,160 - GFF1406 0.70 +0.6 53,175 + GFF1406 0.71 +0.4 53,175 + GFF1406 0.71 +0.2 53,183 + GFF1406 0.72 +0.5 53,187 + GFF1406 0.72 -1.3 53,187 + GFF1406 0.72 -1.3 53,210 + GFF1406 0.74 +0.6 53,210 + GFF1406 0.74 -0.7 53,242 + GFF1406 0.76 -0.5 53,342 + GFF1406 0.83 +0.0 53,342 + GFF1406 0.83 -0.6 53,405 + GFF1406 0.88 +0.4 53,445 + -0.3 53,445 + -0.0 53,566 - -0.4 53,568 + +0.2 53,818 + +0.0 53,818 + +0.1 53,819 - +1.1 53,819 - +0.4 53,819 - +0.5 53,958 + GFF1407 0.25 -0.2 53,977 + GFF1407 0.27 -0.0 53,978 - GFF1407 0.27 -1.4 54,217 - GFF1407 0.53 -0.8 54,246 + GFF1407 0.56 -0.4 54,246 + GFF1407 0.56 +0.2 54,271 + GFF1407 0.59 +0.9 54,289 - GFF1407 0.61 +0.4 54,516 + GFF1407 0.86 +0.2
Or see this region's nucleotide sequence