Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1356 and GFF1357 are separated by 123 nucleotides GFF1357 and GFF1358 are separated by 48 nucleotides GFF1358 and GFF1359 are separated by 266 nucleotides
GFF1356 - Acetate operon repressor, at 7,339 to 8,163
GFF1356
GFF1357 - hypothetical protein, at 8,287 to 8,418
GFF1357
GFF1358 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), at 8,467 to 12,150
GFF1358
GFF1359 - Sodium-dependent phosphate transporter, at 12,417 to 14,048
GFF1359
Position (kb)
8
9
10
11
12
13 Strain fitness (log2 ratio)
-1
0
1 at 7.485 kb on + strand, within GFF1356 at 7.605 kb on + strand, within GFF1356 at 8.214 kb on - strand at 8.310 kb on + strand, within GFF1357 at 8.541 kb on + strand at 8.541 kb on + strand at 8.554 kb on + strand at 8.561 kb on + strand at 8.677 kb on + strand at 8.719 kb on - strand at 8.780 kb on - strand at 8.922 kb on + strand, within GFF1358 at 8.926 kb on - strand, within GFF1358 at 9.160 kb on + strand, within GFF1358 at 9.161 kb on - strand, within GFF1358 at 9.163 kb on - strand, within GFF1358 at 9.201 kb on + strand, within GFF1358 at 9.236 kb on - strand, within GFF1358 at 9.317 kb on - strand, within GFF1358 at 9.417 kb on + strand, within GFF1358 at 9.553 kb on - strand, within GFF1358 at 9.553 kb on - strand, within GFF1358 at 9.604 kb on + strand, within GFF1358 at 9.834 kb on + strand, within GFF1358 at 9.835 kb on - strand, within GFF1358 at 9.980 kb on - strand, within GFF1358 at 10.038 kb on + strand, within GFF1358 at 10.059 kb on + strand, within GFF1358 at 10.078 kb on + strand, within GFF1358 at 10.078 kb on + strand, within GFF1358 at 10.222 kb on + strand, within GFF1358 at 10.279 kb on + strand, within GFF1358 at 10.671 kb on + strand, within GFF1358 at 10.783 kb on - strand, within GFF1358 at 10.783 kb on - strand, within GFF1358 at 10.945 kb on - strand, within GFF1358 at 10.956 kb on - strand, within GFF1358 at 11.032 kb on + strand, within GFF1358 at 11.033 kb on - strand, within GFF1358 at 11.033 kb on - strand, within GFF1358 at 11.125 kb on + strand, within GFF1358 at 11.228 kb on - strand, within GFF1358 at 11.228 kb on - strand, within GFF1358 at 11.296 kb on + strand, within GFF1358 at 11.296 kb on + strand, within GFF1358 at 11.297 kb on - strand, within GFF1358 at 11.297 kb on - strand, within GFF1358 at 11.397 kb on + strand, within GFF1358 at 11.716 kb on - strand, within GFF1358 at 11.716 kb on - strand, within GFF1358 at 11.770 kb on + strand, within GFF1358 at 11.771 kb on - strand, within GFF1358 at 11.787 kb on - strand at 11.916 kb on + strand at 11.927 kb on + strand at 12.073 kb on + strand at 12.181 kb on + strand at 12.181 kb on + strand at 12.181 kb on + strand at 12.181 kb on + strand at 12.182 kb on - strand at 12.182 kb on - strand at 12.215 kb on + strand at 12.216 kb on - strand at 12.230 kb on - strand at 12.232 kb on - strand at 12.297 kb on - strand at 12.297 kb on - strand at 12.299 kb on + strand at 12.299 kb on + strand at 12.303 kb on + strand at 12.391 kb on + strand at 12.394 kb on - strand at 12.473 kb on + strand at 12.474 kb on - strand at 12.474 kb on - strand at 12.701 kb on - strand, within GFF1359 at 12.782 kb on + strand, within GFF1359 at 12.930 kb on - strand, within GFF1359 at 12.966 kb on - strand, within GFF1359 at 13.025 kb on + strand, within GFF1359 at 13.025 kb on + strand, within GFF1359 at 13.027 kb on + strand, within GFF1359 at 13.028 kb on - strand, within GFF1359 at 13.145 kb on - strand, within GFF1359 at 13.145 kb on - strand, within GFF1359
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 7,485 + GFF1356 0.18 -0.5 7,605 + GFF1356 0.32 -0.6 8,214 - -0.1 8,310 + GFF1357 0.17 +0.3 8,541 + -0.4 8,541 + -0.1 8,554 + +0.9 8,561 + -0.0 8,677 + +0.3 8,719 - +0.5 8,780 - -0.2 8,922 + GFF1358 0.12 -0.5 8,926 - GFF1358 0.12 -0.0 9,160 + GFF1358 0.19 -0.1 9,161 - GFF1358 0.19 -0.3 9,163 - GFF1358 0.19 +0.4 9,201 + GFF1358 0.20 +0.2 9,236 - GFF1358 0.21 +0.3 9,317 - GFF1358 0.23 -0.6 9,417 + GFF1358 0.26 -0.6 9,553 - GFF1358 0.29 -0.6 9,553 - GFF1358 0.29 +0.2 9,604 + GFF1358 0.31 +0.6 9,834 + GFF1358 0.37 +0.2 9,835 - GFF1358 0.37 +0.7 9,980 - GFF1358 0.41 -0.3 10,038 + GFF1358 0.43 -0.4 10,059 + GFF1358 0.43 -0.6 10,078 + GFF1358 0.44 +0.4 10,078 + GFF1358 0.44 +0.6 10,222 + GFF1358 0.48 +0.3 10,279 + GFF1358 0.49 -0.4 10,671 + GFF1358 0.60 -0.3 10,783 - GFF1358 0.63 -0.0 10,783 - GFF1358 0.63 -0.2 10,945 - GFF1358 0.67 +0.2 10,956 - GFF1358 0.68 -0.1 11,032 + GFF1358 0.70 +0.6 11,033 - GFF1358 0.70 +0.2 11,033 - GFF1358 0.70 +0.1 11,125 + GFF1358 0.72 +0.4 11,228 - GFF1358 0.75 +0.0 11,228 - GFF1358 0.75 -0.4 11,296 + GFF1358 0.77 +0.2 11,296 + GFF1358 0.77 -0.5 11,297 - GFF1358 0.77 +0.4 11,297 - GFF1358 0.77 +0.2 11,397 + GFF1358 0.80 +0.0 11,716 - GFF1358 0.88 +0.3 11,716 - GFF1358 0.88 +1.5 11,770 + GFF1358 0.90 +0.6 11,771 - GFF1358 0.90 -0.1 11,787 - -0.2 11,916 + +0.2 11,927 + +0.4 12,073 + -0.0 12,181 + +0.3 12,181 + -0.5 12,181 + +0.3 12,181 + -0.6 12,182 - -0.1 12,182 - +0.0 12,215 + +0.2 12,216 - -0.2 12,230 - -0.0 12,232 - +0.3 12,297 - +0.2 12,297 - +0.5 12,299 + +0.2 12,299 + +0.4 12,303 + +0.5 12,391 + -0.1 12,394 - -0.4 12,473 + +0.3 12,474 - -0.3 12,474 - +0.4 12,701 - GFF1359 0.17 +0.2 12,782 + GFF1359 0.22 +0.2 12,930 - GFF1359 0.31 -0.4 12,966 - GFF1359 0.34 -1.6 13,025 + GFF1359 0.37 -0.5 13,025 + GFF1359 0.37 -0.7 13,027 + GFF1359 0.37 +0.2 13,028 - GFF1359 0.37 +0.2 13,145 - GFF1359 0.45 +0.2 13,145 - GFF1359 0.45 +1.5
Or see this region's nucleotide sequence