Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF126 and GFF127 overlap by 1 nucleotides GFF127 and GFF128 are separated by 91 nucleotides GFF128 and GFF129 overlap by 4 nucleotides
GFF126 - Possible GPH family transporter (TC 2.A.2) for arabinosides, at 115,919 to 117,280
GFF126
GFF127 - Alpha-L-arabinofuranosidase II precursor (EC 3.2.1.55), at 117,280 to 118,230
GFF127
GFF128 - AmpE protein, at 118,322 to 119,176
GFF128
GFF129 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 119,173 to 119,736
GFF129
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-1
0
1 at 116.459 kb on - strand, within GFF126 at 116.459 kb on - strand, within GFF126 at 116.569 kb on - strand, within GFF126 at 116.581 kb on - strand, within GFF126 at 116.589 kb on + strand, within GFF126 at 116.589 kb on + strand, within GFF126 at 116.589 kb on + strand, within GFF126 at 116.590 kb on - strand, within GFF126 at 116.590 kb on - strand, within GFF126 at 116.594 kb on - strand, within GFF126 at 116.612 kb on - strand, within GFF126 at 116.633 kb on + strand, within GFF126 at 116.633 kb on + strand, within GFF126 at 116.634 kb on - strand, within GFF126 at 116.634 kb on - strand, within GFF126 at 116.634 kb on - strand, within GFF126 at 116.634 kb on - strand, within GFF126 at 116.636 kb on + strand, within GFF126 at 116.637 kb on - strand, within GFF126 at 116.637 kb on - strand, within GFF126 at 117.094 kb on + strand, within GFF126 at 117.094 kb on + strand, within GFF126 at 117.095 kb on - strand, within GFF126 at 117.095 kb on - strand, within GFF126 at 117.095 kb on - strand, within GFF126 at 117.129 kb on - strand, within GFF126 at 117.151 kb on - strand at 117.328 kb on - strand at 117.333 kb on - strand at 117.333 kb on - strand at 117.349 kb on + strand at 117.353 kb on - strand at 117.357 kb on + strand at 117.451 kb on - strand, within GFF127 at 117.526 kb on + strand, within GFF127 at 117.559 kb on - strand, within GFF127 at 117.897 kb on + strand, within GFF127 at 117.904 kb on + strand, within GFF127 at 117.959 kb on + strand, within GFF127 at 117.993 kb on + strand, within GFF127 at 117.994 kb on - strand, within GFF127 at 118.062 kb on + strand, within GFF127 at 118.228 kb on + strand at 118.328 kb on + strand at 118.328 kb on + strand at 118.339 kb on - strand at 118.418 kb on + strand, within GFF128 at 118.479 kb on + strand, within GFF128 at 118.479 kb on + strand, within GFF128 at 118.489 kb on + strand, within GFF128 at 118.490 kb on - strand, within GFF128 at 118.490 kb on - strand, within GFF128 at 118.554 kb on + strand, within GFF128 at 118.554 kb on + strand, within GFF128 at 118.560 kb on - strand, within GFF128 at 118.808 kb on - strand, within GFF128 at 118.808 kb on - strand, within GFF128 at 118.905 kb on + strand, within GFF128 at 118.906 kb on - strand, within GFF128 at 118.911 kb on - strand, within GFF128 at 118.934 kb on - strand, within GFF128 at 118.983 kb on - strand, within GFF128 at 118.994 kb on + strand, within GFF128 at 119.084 kb on + strand, within GFF128 at 119.156 kb on + strand at 119.164 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 116,459 - GFF126 0.40 -0.4 116,459 - GFF126 0.40 +0.1 116,569 - GFF126 0.48 +0.5 116,581 - GFF126 0.49 +0.3 116,589 + GFF126 0.49 -0.5 116,589 + GFF126 0.49 -1.5 116,589 + GFF126 0.49 -0.1 116,590 - GFF126 0.49 -0.5 116,590 - GFF126 0.49 -0.5 116,594 - GFF126 0.50 -0.5 116,612 - GFF126 0.51 +0.4 116,633 + GFF126 0.52 -0.8 116,633 + GFF126 0.52 -0.2 116,634 - GFF126 0.52 +0.4 116,634 - GFF126 0.52 +0.4 116,634 - GFF126 0.52 +0.3 116,634 - GFF126 0.52 -0.3 116,636 + GFF126 0.53 +0.0 116,637 - GFF126 0.53 +0.5 116,637 - GFF126 0.53 +0.1 117,094 + GFF126 0.86 +0.0 117,094 + GFF126 0.86 -0.2 117,095 - GFF126 0.86 +0.3 117,095 - GFF126 0.86 -0.0 117,095 - GFF126 0.86 +0.2 117,129 - GFF126 0.89 +0.3 117,151 - -0.2 117,328 - -0.1 117,333 - +1.0 117,333 - -0.1 117,349 + +0.3 117,353 - +0.1 117,357 + +0.4 117,451 - GFF127 0.18 -0.5 117,526 + GFF127 0.26 +0.2 117,559 - GFF127 0.29 +0.1 117,897 + GFF127 0.65 -0.0 117,904 + GFF127 0.66 +0.2 117,959 + GFF127 0.71 -0.2 117,993 + GFF127 0.75 +0.3 117,994 - GFF127 0.75 -0.3 118,062 + GFF127 0.82 +0.2 118,228 + +0.2 118,328 + -1.4 118,328 + -0.1 118,339 - +0.0 118,418 + GFF128 0.11 +0.2 118,479 + GFF128 0.18 -0.7 118,479 + GFF128 0.18 -0.6 118,489 + GFF128 0.20 +0.2 118,490 - GFF128 0.20 +0.5 118,490 - GFF128 0.20 +1.0 118,554 + GFF128 0.27 +0.3 118,554 + GFF128 0.27 -0.3 118,560 - GFF128 0.28 -0.1 118,808 - GFF128 0.57 -0.2 118,808 - GFF128 0.57 -0.8 118,905 + GFF128 0.68 -0.3 118,906 - GFF128 0.68 -0.1 118,911 - GFF128 0.69 -0.1 118,934 - GFF128 0.72 +0.0 118,983 - GFF128 0.77 -0.0 118,994 + GFF128 0.79 +0.1 119,084 + GFF128 0.89 +0.7 119,156 + +0.1 119,164 + +0.1
Or see this region's nucleotide sequence