Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1245 and GFF1246 are separated by 421 nucleotides GFF1246 and GFF1247 are separated by 344 nucleotides
GFF1245 - FIG01046087: hypothetical protein, at 75,918 to 76,556
GFF1245
GFF1246 - RlgA, at 76,978 to 77,400
GFF1246
GFF1247 - Carbonic anhydrase (EC 4.2.1.1), at 77,745 to 78,485
GFF1247
Position (kb) 76
77
78 Strain fitness (log2 ratio) -2
-1
0
1 at 76.018 kb on + strand, within GFF1245 at 76.031 kb on - strand, within GFF1245 at 76.182 kb on - strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.227 kb on + strand, within GFF1245 at 76.228 kb on - strand, within GFF1245 at 76.262 kb on - strand, within GFF1245 at 76.262 kb on - strand, within GFF1245 at 76.360 kb on + strand, within GFF1245 at 76.360 kb on + strand, within GFF1245 at 76.361 kb on - strand, within GFF1245 at 76.372 kb on + strand, within GFF1245 at 76.408 kb on - strand, within GFF1245 at 76.494 kb on - strand at 76.555 kb on - strand at 76.617 kb on + strand at 76.634 kb on + strand at 76.729 kb on - strand at 76.869 kb on - strand at 76.873 kb on + strand at 76.873 kb on + strand at 76.873 kb on + strand at 76.947 kb on + strand at 76.947 kb on + strand at 77.012 kb on + strand at 77.076 kb on + strand, within GFF1246 at 77.078 kb on + strand, within GFF1246 at 77.078 kb on + strand, within GFF1246 at 77.079 kb on - strand, within GFF1246 at 77.079 kb on - strand, within GFF1246 at 77.087 kb on + strand, within GFF1246 at 77.088 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.090 kb on - strand, within GFF1246 at 77.105 kb on - strand, within GFF1246 at 77.105 kb on - strand, within GFF1246 at 77.127 kb on - strand, within GFF1246 at 77.128 kb on + strand, within GFF1246 at 77.215 kb on - strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.300 kb on + strand, within GFF1246 at 77.301 kb on - strand, within GFF1246 at 77.301 kb on - strand, within GFF1246 at 77.365 kb on - strand at 77.449 kb on - strand at 77.491 kb on - strand at 77.528 kb on + strand at 77.529 kb on - strand at 77.539 kb on - strand at 77.567 kb on - strand at 77.700 kb on + strand at 77.747 kb on - strand at 77.786 kb on - strand at 77.789 kb on + strand at 77.790 kb on - strand at 77.916 kb on - strand, within GFF1247 at 77.922 kb on - strand, within GFF1247 at 78.002 kb on + strand, within GFF1247 at 78.003 kb on - strand, within GFF1247 at 78.102 kb on - strand, within GFF1247 at 78.117 kb on - strand, within GFF1247 at 78.201 kb on - strand, within GFF1247 at 78.305 kb on + strand, within GFF1247
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 76,018 + GFF1245 0.16 -0.8 76,031 - GFF1245 0.18 -0.0 76,182 - GFF1245 0.41 -0.3 76,227 + GFF1245 0.48 +0.6 76,227 + GFF1245 0.48 +0.5 76,227 + GFF1245 0.48 -0.4 76,228 - GFF1245 0.49 -1.3 76,262 - GFF1245 0.54 -0.4 76,262 - GFF1245 0.54 -0.3 76,360 + GFF1245 0.69 +0.4 76,360 + GFF1245 0.69 -0.3 76,361 - GFF1245 0.69 -0.4 76,372 + GFF1245 0.71 -0.1 76,408 - GFF1245 0.77 +0.3 76,494 - +0.3 76,555 - -0.4 76,617 + -0.1 76,634 + +0.3 76,729 - -1.4 76,869 - -0.2 76,873 + -0.3 76,873 + -0.5 76,873 + -0.0 76,947 + -0.1 76,947 + -0.8 77,012 + -1.2 77,076 + GFF1246 0.23 -0.2 77,078 + GFF1246 0.24 -0.4 77,078 + GFF1246 0.24 -0.4 77,079 - GFF1246 0.24 -0.0 77,079 - GFF1246 0.24 -1.6 77,087 + GFF1246 0.26 +0.2 77,088 - GFF1246 0.26 -0.0 77,090 - GFF1246 0.26 +0.0 77,090 - GFF1246 0.26 +0.5 77,090 - GFF1246 0.26 -0.2 77,105 - GFF1246 0.30 +0.6 77,105 - GFF1246 0.30 -0.7 77,127 - GFF1246 0.35 +0.1 77,128 + GFF1246 0.35 -0.7 77,215 - GFF1246 0.56 -1.6 77,300 + GFF1246 0.76 -0.0 77,300 + GFF1246 0.76 -0.3 77,300 + GFF1246 0.76 -0.3 77,301 - GFF1246 0.76 +0.2 77,301 - GFF1246 0.76 +0.1 77,365 - -0.0 77,449 - +0.0 77,491 - -1.8 77,528 + +0.0 77,529 - +0.0 77,539 - -0.6 77,567 - -0.4 77,700 + +0.7 77,747 - -0.4 77,786 - -0.5 77,789 + -1.0 77,790 - +0.1 77,916 - GFF1247 0.23 -0.2 77,922 - GFF1247 0.24 -0.9 78,002 + GFF1247 0.35 -0.9 78,003 - GFF1247 0.35 +0.1 78,102 - GFF1247 0.48 -0.3 78,117 - GFF1247 0.50 -0.1 78,201 - GFF1247 0.62 +0.2 78,305 + GFF1247 0.76 +0.2
Or see this region's nucleotide sequence