Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1101 and GFF1102 are separated by 0 nucleotides GFF1102 and GFF1103 are separated by 30 nucleotides GFF1103 and GFF1104 are separated by 15 nucleotides
GFF1101 - type 1 fimbriae anchoring protein FimD, at 286,587 to 289,208
GFF1101
GFF1102 - Fimbriae-like adhesin SfmH, at 289,209 to 290,216
GFF1102
GFF1103 - type 1 fimbrae adaptor subunit FimF, at 290,247 to 290,762
GFF1103
GFF1104 - type 1 fimbrae adaptor subunit FimF, at 290,778 to 291,296
GFF1104
Position (kb)
289
290
291 Strain fitness (log2 ratio)
-1
0
1
2
3 at 288.312 kb on + strand, within GFF1101 at 288.432 kb on + strand, within GFF1101 at 288.452 kb on + strand, within GFF1101 at 288.467 kb on + strand, within GFF1101 at 288.661 kb on - strand, within GFF1101 at 288.792 kb on - strand, within GFF1101 at 289.392 kb on - strand, within GFF1102 at 289.392 kb on - strand, within GFF1102 at 289.392 kb on - strand, within GFF1102 at 289.441 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.450 kb on - strand, within GFF1102 at 289.494 kb on + strand, within GFF1102 at 289.515 kb on + strand, within GFF1102 at 289.706 kb on + strand, within GFF1102 at 289.707 kb on - strand, within GFF1102 at 289.712 kb on + strand, within GFF1102 at 289.712 kb on + strand, within GFF1102 at 289.719 kb on + strand, within GFF1102 at 289.761 kb on + strand, within GFF1102 at 289.775 kb on + strand, within GFF1102 at 290.094 kb on - strand, within GFF1102 at 290.148 kb on - strand at 290.322 kb on - strand, within GFF1103 at 290.344 kb on + strand, within GFF1103 at 290.344 kb on + strand, within GFF1103 at 290.618 kb on + strand, within GFF1103 at 290.679 kb on + strand, within GFF1103 at 290.773 kb on + strand at 290.789 kb on + strand at 291.060 kb on - strand, within GFF1104 at 291.071 kb on - strand, within GFF1104 at 291.071 kb on - strand, within GFF1104 at 291.134 kb on + strand, within GFF1104
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 288,312 + GFF1101 0.66 +0.1 288,432 + GFF1101 0.70 +0.4 288,452 + GFF1101 0.71 +0.1 288,467 + GFF1101 0.72 +0.4 288,661 - GFF1101 0.79 +0.2 288,792 - GFF1101 0.84 +0.5 289,392 - GFF1102 0.18 -1.4 289,392 - GFF1102 0.18 +0.3 289,392 - GFF1102 0.18 -0.1 289,441 - GFF1102 0.23 -0.3 289,450 - GFF1102 0.24 +0.4 289,450 - GFF1102 0.24 -0.6 289,450 - GFF1102 0.24 -1.2 289,450 - GFF1102 0.24 -0.2 289,494 + GFF1102 0.28 -0.2 289,515 + GFF1102 0.30 +0.3 289,706 + GFF1102 0.49 -0.2 289,707 - GFF1102 0.49 +0.2 289,712 + GFF1102 0.50 -0.8 289,712 + GFF1102 0.50 -0.4 289,719 + GFF1102 0.51 -0.6 289,761 + GFF1102 0.55 +0.4 289,775 + GFF1102 0.56 +0.4 290,094 - GFF1102 0.88 +0.1 290,148 - -0.2 290,322 - GFF1103 0.15 -0.8 290,344 + GFF1103 0.19 +0.1 290,344 + GFF1103 0.19 +0.3 290,618 + GFF1103 0.72 +0.5 290,679 + GFF1103 0.84 +0.5 290,773 + +0.9 290,789 + -0.1 291,060 - GFF1104 0.54 +3.1 291,071 - GFF1104 0.56 +0.5 291,071 - GFF1104 0.56 +0.6 291,134 + GFF1104 0.69 +0.0
Or see this region's nucleotide sequence