Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1048 and GFF1049 are separated by 13 nucleotides GFF1049 and GFF1050 are separated by 77 nucleotides GFF1050 and GFF1051 are separated by 47 nucleotides
GFF1048 - Beta-fimbriae usher protein, at 229,819 to 232,227
GFF1048
GFF1049 - Beta-fimbriae chaperone protein, at 232,241 to 232,948
GFF1049
GFF1050 - Beta-fimbriae probable major subunit, at 233,026 to 233,715
GFF1050
GFF1051 - Beta-fimbriae probable major subunit, at 233,763 to 234,452
GFF1051
Position (kb) 232
233 Strain fitness (log2 ratio) -2
-1
0
1 at 231.310 kb on - strand, within GFF1048 at 231.352 kb on - strand, within GFF1048 at 231.435 kb on + strand, within GFF1048 at 231.449 kb on - strand, within GFF1048 at 231.486 kb on + strand, within GFF1048 at 231.520 kb on + strand, within GFF1048 at 231.594 kb on + strand, within GFF1048 at 231.594 kb on + strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.598 kb on + strand, within GFF1048 at 231.599 kb on - strand, within GFF1048 at 231.599 kb on - strand, within GFF1048 at 231.627 kb on + strand, within GFF1048 at 231.673 kb on + strand, within GFF1048 at 231.751 kb on - strand, within GFF1048 at 231.757 kb on - strand, within GFF1048 at 231.799 kb on + strand, within GFF1048 at 231.802 kb on + strand, within GFF1048 at 231.808 kb on - strand, within GFF1048 at 231.870 kb on + strand, within GFF1048 at 231.896 kb on - strand, within GFF1048 at 232.058 kb on - strand at 232.202 kb on + strand at 232.202 kb on + strand at 232.203 kb on - strand at 232.203 kb on - strand at 232.203 kb on - strand at 232.203 kb on - strand at 232.205 kb on - strand at 232.340 kb on - strand, within GFF1049 at 232.511 kb on - strand, within GFF1049 at 232.541 kb on + strand, within GFF1049 at 232.630 kb on - strand, within GFF1049 at 232.673 kb on + strand, within GFF1049 at 232.766 kb on - strand, within GFF1049 at 233.038 kb on - strand at 233.260 kb on - strand, within GFF1050 at 233.310 kb on + strand, within GFF1050 at 233.311 kb on - strand, within GFF1050 at 233.380 kb on + strand, within GFF1050 at 233.380 kb on + strand, within GFF1050 at 233.380 kb on + strand, within GFF1050 at 233.382 kb on + strand, within GFF1050 at 233.382 kb on + strand, within GFF1050 at 233.383 kb on - strand, within GFF1050 at 233.385 kb on + strand, within GFF1050 at 233.386 kb on - strand, within GFF1050 at 233.386 kb on - strand, within GFF1050 at 233.388 kb on + strand, within GFF1050 at 233.389 kb on - strand, within GFF1050 at 233.389 kb on - strand, within GFF1050 at 233.389 kb on - strand, within GFF1050 at 233.457 kb on + strand, within GFF1050 at 233.494 kb on - strand, within GFF1050 at 233.512 kb on - strand, within GFF1050 at 233.512 kb on - strand, within GFF1050 at 233.512 kb on - strand, within GFF1050 at 233.638 kb on + strand, within GFF1050 at 233.770 kb on + strand at 233.772 kb on + strand at 233.781 kb on - strand at 233.841 kb on + strand, within GFF1051 at 233.854 kb on - strand, within GFF1051 at 233.880 kb on - strand, within GFF1051 at 233.913 kb on - strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.914 kb on + strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.915 kb on - strand, within GFF1051 at 233.918 kb on + strand, within GFF1051 at 233.918 kb on + strand, within GFF1051 at 233.937 kb on - strand, within GFF1051 at 233.943 kb on - strand, within GFF1051
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 231,310 - GFF1048 0.62 +0.2 231,352 - GFF1048 0.64 +0.2 231,435 + GFF1048 0.67 -1.7 231,449 - GFF1048 0.68 +1.1 231,486 + GFF1048 0.69 +0.3 231,520 + GFF1048 0.71 +1.3 231,594 + GFF1048 0.74 -0.2 231,594 + GFF1048 0.74 +0.3 231,595 - GFF1048 0.74 +1.6 231,595 - GFF1048 0.74 -0.0 231,595 - GFF1048 0.74 +0.3 231,595 - GFF1048 0.74 +0.2 231,598 + GFF1048 0.74 -0.1 231,599 - GFF1048 0.74 -1.7 231,599 - GFF1048 0.74 +0.4 231,627 + GFF1048 0.75 -0.2 231,673 + GFF1048 0.77 -0.1 231,751 - GFF1048 0.80 +0.2 231,757 - GFF1048 0.80 +0.7 231,799 + GFF1048 0.82 -0.8 231,802 + GFF1048 0.82 -0.1 231,808 - GFF1048 0.83 +0.9 231,870 + GFF1048 0.85 -0.3 231,896 - GFF1048 0.86 -0.2 232,058 - -0.0 232,202 + -0.1 232,202 + -0.0 232,203 - +0.5 232,203 - -0.1 232,203 - +0.4 232,203 - -0.4 232,205 - +1.0 232,340 - GFF1049 0.14 -0.0 232,511 - GFF1049 0.38 -0.3 232,541 + GFF1049 0.42 -0.0 232,630 - GFF1049 0.55 -0.3 232,673 + GFF1049 0.61 -0.2 232,766 - GFF1049 0.74 -2.0 233,038 - +1.5 233,260 - GFF1050 0.34 +0.3 233,310 + GFF1050 0.41 +0.1 233,311 - GFF1050 0.41 -0.3 233,380 + GFF1050 0.51 +0.0 233,380 + GFF1050 0.51 +0.7 233,380 + GFF1050 0.51 -0.2 233,382 + GFF1050 0.52 -0.4 233,382 + GFF1050 0.52 -0.4 233,383 - GFF1050 0.52 -0.2 233,385 + GFF1050 0.52 -0.4 233,386 - GFF1050 0.52 -0.2 233,386 - GFF1050 0.52 +0.2 233,388 + GFF1050 0.52 -0.1 233,389 - GFF1050 0.53 -0.0 233,389 - GFF1050 0.53 -0.1 233,389 - GFF1050 0.53 +0.9 233,457 + GFF1050 0.62 +0.1 233,494 - GFF1050 0.68 +0.5 233,512 - GFF1050 0.70 -0.8 233,512 - GFF1050 0.70 +0.7 233,512 - GFF1050 0.70 -1.9 233,638 + GFF1050 0.89 +0.1 233,770 + -0.1 233,772 + -0.4 233,781 - +0.5 233,841 + GFF1051 0.11 -0.3 233,854 - GFF1051 0.13 -1.1 233,880 - GFF1051 0.17 -0.3 233,913 - GFF1051 0.22 +0.4 233,914 + GFF1051 0.22 -1.2 233,914 + GFF1051 0.22 -0.3 233,914 + GFF1051 0.22 -0.2 233,915 - GFF1051 0.22 +0.5 233,915 - GFF1051 0.22 -0.2 233,915 - GFF1051 0.22 -0.1 233,918 + GFF1051 0.22 +0.8 233,918 + GFF1051 0.22 +0.5 233,937 - GFF1051 0.25 +0.6 233,943 - GFF1051 0.26 +0.6
Or see this region's nucleotide sequence