Experiment: LB_plus_SM_buffer with 6D2_phage 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1019 and GFF1020 are separated by 57 nucleotides GFF1020 and GFF1021 are separated by 2 nucleotides GFF1021 and GFF1022 are separated by 92 nucleotides GFF1022 and GFF1023 overlap by 10 nucleotides
GFF1019 - Putative glycoprotein/receptor, at 205,625 to 206,086
GFF1019
GFF1020 - DNA replication protein DnaC, at 206,144 to 206,881
GFF1020
GFF1021 - Primosomal protein I, at 206,884 to 207,423
GFF1021
GFF1022 - FIG001826: putative inner membrane protein, at 207,516 to 207,989
GFF1022
GFF1023 - FIG023911: putative membrane protein, at 207,980 to 208,924
GFF1023
Position (kb) 206
207
208 Strain fitness (log2 ratio) -2
-1
0
1 at 205.900 kb on + strand, within GFF1019 at 205.901 kb on - strand, within GFF1019 at 206.040 kb on - strand at 206.040 kb on - strand at 206.080 kb on + strand at 206.080 kb on + strand at 206.081 kb on - strand at 206.090 kb on - strand at 207.424 kb on - strand at 207.424 kb on - strand at 207.490 kb on - strand at 207.490 kb on - strand at 207.547 kb on - strand at 207.683 kb on - strand, within GFF1022 at 207.842 kb on + strand, within GFF1022 at 207.969 kb on + strand at 207.981 kb on + strand at 207.981 kb on + strand at 207.983 kb on + strand at 207.984 kb on - strand at 208.004 kb on + strand at 208.084 kb on + strand, within GFF1023 at 208.084 kb on + strand, within GFF1023 at 208.085 kb on - strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.087 kb on + strand, within GFF1023 at 208.088 kb on - strand, within GFF1023 at 208.088 kb on - strand, within GFF1023 at 208.103 kb on - strand, within GFF1023 at 208.117 kb on + strand, within GFF1023 at 208.148 kb on + strand, within GFF1023 at 208.149 kb on - strand, within GFF1023 at 208.293 kb on + strand, within GFF1023 at 208.293 kb on + strand, within GFF1023 at 208.294 kb on - strand, within GFF1023 at 208.294 kb on - strand, within GFF1023 at 208.294 kb on - strand, within GFF1023 at 208.301 kb on + strand, within GFF1023
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 0.75 MOI remove 205,900 + GFF1019 0.60 -0.3 205,901 - GFF1019 0.60 -0.1 206,040 - +0.1 206,040 - -0.6 206,080 + -0.5 206,080 + +0.4 206,081 - -0.0 206,090 - -0.4 207,424 - +0.5 207,424 - -0.4 207,490 - +0.2 207,490 - +0.1 207,547 - +0.2 207,683 - GFF1022 0.35 -0.4 207,842 + GFF1022 0.69 +0.1 207,969 + -0.6 207,981 + -0.1 207,981 + +0.5 207,983 + +0.4 207,984 - +0.0 208,004 + -1.4 208,084 + GFF1023 0.11 +0.3 208,084 + GFF1023 0.11 -0.3 208,085 - GFF1023 0.11 +0.1 208,087 + GFF1023 0.11 -0.1 208,087 + GFF1023 0.11 +0.2 208,087 + GFF1023 0.11 +0.2 208,087 + GFF1023 0.11 -1.0 208,088 - GFF1023 0.11 +0.2 208,088 - GFF1023 0.11 +0.3 208,103 - GFF1023 0.13 +0.2 208,117 + GFF1023 0.14 +0.0 208,148 + GFF1023 0.18 -0.2 208,149 - GFF1023 0.18 +0.1 208,293 + GFF1023 0.33 +0.3 208,293 + GFF1023 0.33 +0.2 208,294 - GFF1023 0.33 +0.2 208,294 - GFF1023 0.33 -1.9 208,294 - GFF1023 0.33 -0.7 208,301 + GFF1023 0.34 +0.1
Or see this region's nucleotide sequence