Experiment: 3-methyl-3-butenol 0.625 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagE and yagF are separated by 14 nucleotides yagF and yagG are separated by 226 nucleotides
b0268: yagE - 2-keto-3-deoxy gluconate (KDG) aldolase; CP4-6 prophage (NCBI), at 281,502 to 282,410
yagE
b0269: yagF - CP4-6 prophage; predicted dehydratase (NCBI), at 282,425 to 284,392
yagF
b0270: yagG - CP4-6 prophage; predicted sugar transporter (NCBI), at 284,619 to 286,001
yagG
Position (kb) 282
283
284
285 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 281.464 kb on + strand at 281.464 kb on + strand at 281.579 kb on + strand at 281.579 kb on + strand at 281.639 kb on - strand, within yagE at 281.664 kb on + strand, within yagE at 281.869 kb on + strand, within yagE at 282.021 kb on - strand, within yagE at 282.021 kb on - strand, within yagE at 282.072 kb on + strand, within yagE at 282.101 kb on + strand, within yagE at 282.109 kb on - strand, within yagE at 282.109 kb on - strand, within yagE at 282.114 kb on - strand, within yagE at 282.114 kb on - strand, within yagE at 282.297 kb on + strand, within yagE at 282.305 kb on + strand, within yagE at 282.321 kb on - strand at 282.321 kb on - strand at 282.449 kb on + strand at 282.524 kb on - strand at 282.524 kb on - strand at 282.553 kb on + strand at 282.591 kb on + strand at 282.684 kb on - strand, within yagF at 282.811 kb on + strand, within yagF at 282.811 kb on + strand, within yagF at 282.958 kb on - strand, within yagF at 283.075 kb on - strand, within yagF at 283.090 kb on - strand, within yagF at 283.154 kb on - strand, within yagF at 283.393 kb on + strand, within yagF at 283.411 kb on - strand, within yagF at 283.411 kb on - strand, within yagF at 283.434 kb on + strand, within yagF at 283.456 kb on - strand, within yagF at 283.584 kb on + strand, within yagF at 283.584 kb on + strand, within yagF at 283.731 kb on - strand, within yagF at 283.820 kb on + strand, within yagF at 283.835 kb on + strand, within yagF at 283.835 kb on + strand, within yagF at 284.022 kb on - strand, within yagF at 284.115 kb on - strand, within yagF at 284.150 kb on + strand, within yagF at 284.237 kb on + strand at 284.237 kb on + strand at 284.251 kb on - strand at 284.252 kb on + strand at 284.274 kb on + strand at 284.274 kb on + strand at 284.281 kb on + strand at 284.281 kb on + strand at 284.281 kb on + strand at 284.281 kb on + strand at 284.289 kb on - strand at 284.290 kb on - strand at 284.291 kb on + strand at 284.291 kb on + strand at 284.291 kb on + strand at 284.292 kb on - strand at 284.294 kb on - strand at 284.307 kb on + strand at 284.307 kb on + strand at 284.311 kb on - strand at 284.311 kb on - strand at 284.316 kb on + strand at 284.328 kb on + strand at 284.330 kb on + strand at 284.417 kb on + strand at 284.565 kb on + strand at 284.582 kb on - strand at 284.590 kb on - strand at 284.596 kb on + strand at 284.598 kb on + strand at 284.598 kb on + strand at 284.598 kb on + strand at 284.598 kb on + strand at 284.598 kb on + strand at 284.598 kb on + strand at 284.598 kb on + strand at 284.604 kb on - strand at 284.606 kb on - strand at 284.606 kb on - strand at 284.606 kb on - strand at 284.606 kb on - strand at 284.606 kb on - strand at 284.606 kb on - strand at 284.608 kb on - strand at 284.608 kb on - strand at 284.608 kb on - strand at 284.608 kb on - strand at 284.608 kb on - strand at 284.610 kb on + strand at 284.610 kb on + strand at 284.616 kb on - strand at 284.655 kb on + strand at 284.658 kb on - strand at 284.879 kb on + strand, within yagG at 284.879 kb on + strand, within yagG at 284.910 kb on + strand, within yagG at 284.938 kb on - strand, within yagG at 284.955 kb on + strand, within yagG at 284.975 kb on + strand, within yagG at 285.007 kb on - strand, within yagG at 285.007 kb on - strand, within yagG at 285.059 kb on - strand, within yagG at 285.190 kb on + strand, within yagG at 285.208 kb on - strand, within yagG at 285.246 kb on - strand, within yagG
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol 0.625 vol% remove 281,464 + +0.3 281,464 + +0.5 281,579 + -0.3 281,579 + -1.9 281,639 - yagE b0268 0.15 +0.2 281,664 + yagE b0268 0.18 +1.1 281,869 + yagE b0268 0.40 -0.2 282,021 - yagE b0268 0.57 -0.9 282,021 - yagE b0268 0.57 -0.2 282,072 + yagE b0268 0.63 -0.1 282,101 + yagE b0268 0.66 +0.1 282,109 - yagE b0268 0.67 -1.3 282,109 - yagE b0268 0.67 -1.1 282,114 - yagE b0268 0.67 +0.6 282,114 - yagE b0268 0.67 +0.2 282,297 + yagE b0268 0.87 +0.2 282,305 + yagE b0268 0.88 +0.5 282,321 - +0.9 282,321 - +1.6 282,449 + +0.1 282,524 - -0.9 282,524 - -1.1 282,553 + +0.3 282,591 + +0.3 282,684 - yagF b0269 0.13 +1.2 282,811 + yagF b0269 0.20 -0.9 282,811 + yagF b0269 0.20 +1.0 282,958 - yagF b0269 0.27 -0.0 283,075 - yagF b0269 0.33 +0.5 283,090 - yagF b0269 0.34 +0.7 283,154 - yagF b0269 0.37 +0.6 283,393 + yagF b0269 0.49 -0.5 283,411 - yagF b0269 0.50 -0.3 283,411 - yagF b0269 0.50 -1.1 283,434 + yagF b0269 0.51 -0.1 283,456 - yagF b0269 0.52 +0.4 283,584 + yagF b0269 0.59 +0.1 283,584 + yagF b0269 0.59 +0.6 283,731 - yagF b0269 0.66 -0.1 283,820 + yagF b0269 0.71 +0.1 283,835 + yagF b0269 0.72 +0.3 283,835 + yagF b0269 0.72 +0.1 284,022 - yagF b0269 0.81 -0.1 284,115 - yagF b0269 0.86 +2.8 284,150 + yagF b0269 0.88 -0.1 284,237 + +0.1 284,237 + +0.4 284,251 - +0.1 284,252 + +0.3 284,274 + +0.5 284,274 + -0.1 284,281 + -0.1 284,281 + +1.3 284,281 + +0.2 284,281 + -0.3 284,289 - +0.6 284,290 - -0.2 284,291 + +0.6 284,291 + -0.6 284,291 + -0.9 284,292 - -0.3 284,294 - +0.2 284,307 + +0.1 284,307 + -0.9 284,311 - -0.6 284,311 - -0.2 284,316 + +0.7 284,328 + -0.2 284,330 + +0.5 284,417 + +0.3 284,565 + -0.1 284,582 - +0.3 284,590 - +0.1 284,596 + -0.1 284,598 + +0.6 284,598 + +0.4 284,598 + -0.0 284,598 + -0.1 284,598 + +0.9 284,598 + -0.2 284,598 + +0.5 284,604 - -0.2 284,606 - -0.6 284,606 - +0.1 284,606 - -0.4 284,606 - -0.2 284,606 - +0.0 284,606 - -0.0 284,608 - +0.0 284,608 - +0.4 284,608 - +0.0 284,608 - +0.0 284,608 - +0.2 284,610 + -0.1 284,610 + -0.7 284,616 - +0.6 284,655 + -0.7 284,658 - +0.8 284,879 + yagG b0270 0.19 -0.3 284,879 + yagG b0270 0.19 -0.3 284,910 + yagG b0270 0.21 -0.6 284,938 - yagG b0270 0.23 -0.2 284,955 + yagG b0270 0.24 +0.3 284,975 + yagG b0270 0.26 +0.9 285,007 - yagG b0270 0.28 +0.8 285,007 - yagG b0270 0.28 -0.0 285,059 - yagG b0270 0.32 +0.2 285,190 + yagG b0270 0.41 -0.5 285,208 - yagG b0270 0.43 -0.6 285,246 - yagG b0270 0.45 -0.6
Or see this region's nucleotide sequence