Experiment: 3-methyl-3-butenol 0.625 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafS and rnhA overlap by 4 nucleotides rnhA and dnaQ are separated by 64 nucleotides dnaQ and aspV are separated by 132 nucleotides aspV and yafT are separated by 327 nucleotides
b0213: yafS - orf, hypothetical protein (VIMSS), at 234,816 to 235,538
yafS
b0214: rnhA - ribonuclease H (NCBI), at 235,535 to 236,002
rnhA
b0215: dnaQ - DNA polymerase III subunit epsilon (NCBI), at 236,067 to 236,798
dnaQ
b0216: aspV - tRNA-Asp (NCBI), at 236,931 to 237,007
aspV
b0217: yafT - predicted aminopeptidase (NCBI), at 237,335 to 238,120
yafT
Position (kb) 236
237 Strain fitness (log2 ratio) -1
0
1 at 235.257 kb on + strand, within yafS at 235.257 kb on + strand, within yafS at 235.325 kb on + strand, within yafS at 235.333 kb on - strand, within yafS at 235.379 kb on + strand, within yafS at 235.519 kb on + strand at 235.527 kb on - strand at 235.569 kb on - strand at 235.569 kb on - strand at 236.069 kb on + strand at 236.072 kb on - strand at 236.876 kb on + strand at 236.876 kb on + strand at 236.910 kb on + strand at 236.910 kb on + strand at 236.917 kb on + strand at 236.965 kb on + strand, within aspV at 236.965 kb on + strand, within aspV at 236.968 kb on + strand, within aspV at 236.985 kb on + strand, within aspV at 236.985 kb on + strand, within aspV at 236.993 kb on + strand, within aspV at 236.994 kb on + strand, within aspV at 237.008 kb on + strand at 237.008 kb on + strand at 237.027 kb on + strand at 237.027 kb on + strand at 237.110 kb on + strand at 237.110 kb on + strand at 237.122 kb on + strand at 237.131 kb on + strand at 237.163 kb on + strand at 237.576 kb on - strand, within yafT at 237.576 kb on - strand, within yafT at 237.619 kb on - strand, within yafT at 237.669 kb on - strand, within yafT at 237.674 kb on - strand, within yafT at 237.687 kb on + strand, within yafT at 237.689 kb on - strand, within yafT at 237.689 kb on - strand, within yafT at 237.741 kb on + strand, within yafT at 237.775 kb on + strand, within yafT
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol 0.625 vol% remove 235,257 + yafS b0213 0.61 +0.5 235,257 + yafS b0213 0.61 -0.1 235,325 + yafS b0213 0.70 -0.2 235,333 - yafS b0213 0.72 +0.9 235,379 + yafS b0213 0.78 +0.6 235,519 + -0.1 235,527 - +0.1 235,569 - -0.3 235,569 - -0.9 236,069 + -0.6 236,072 - +0.4 236,876 + -1.0 236,876 + +0.8 236,910 + +0.5 236,910 + +0.1 236,917 + -0.1 236,965 + aspV b0216 0.44 -0.0 236,965 + aspV b0216 0.44 -0.4 236,968 + aspV b0216 0.48 -0.2 236,985 + aspV b0216 0.70 +0.2 236,985 + aspV b0216 0.70 +0.8 236,993 + aspV b0216 0.81 -0.9 236,994 + aspV b0216 0.82 -0.7 237,008 + +0.3 237,008 + +1.3 237,027 + -0.6 237,027 + -1.0 237,110 + +0.1 237,110 + -0.3 237,122 + -1.1 237,131 + +0.1 237,163 + +0.8 237,576 - yafT b0217 0.31 -0.4 237,576 - yafT b0217 0.31 +0.9 237,619 - yafT b0217 0.36 -0.0 237,669 - yafT b0217 0.42 +0.2 237,674 - yafT b0217 0.43 -0.3 237,687 + yafT b0217 0.45 +0.5 237,689 - yafT b0217 0.45 -0.1 237,689 - yafT b0217 0.45 +0.1 237,741 + yafT b0217 0.52 +1.2 237,775 + yafT b0217 0.56 -0.5
Or see this region's nucleotide sequence