Strain Fitness in Escherichia coli BW25113 around b3770

Experiment: 3-methyl-3-butenol 0.625 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-3-butenol 0.625 vol%
3,949,513	-	ilvG	b4488	0.57	-0.4
3,949,533	+	ilvG	b4488	0.58	+1.8
3,949,533	+	ilvG	b4488	0.58	-0.4
3,949,533	+	ilvG	b4488	0.58	-0.9
3,949,566	+	ilvG	b4488	0.60	-0.4
3,949,566	+	ilvG	b4488	0.60	+0.1
3,949,571	+	ilvG	b4488	0.60	+1.3
3,949,571	+	ilvG	b4488	0.60	+0.1
3,949,577	-	ilvG	b4488	0.60	+0.4
3,949,618	-	ilvG	b4488	0.63	-0.7
3,949,714	+	ilvG	b4488	0.69	+1.0
3,949,717	+	ilvG	b4488	0.69	+0.6
3,949,722	+	ilvG	b4488	0.69	-0.1
3,949,722	+	ilvG	b4488	0.69	-1.5
3,949,725	-	ilvG	b4488	0.69	+0.1
3,949,725	-	ilvG	b4488	0.69	-1.9
3,949,725	-	ilvG	b4488	0.69	+0.6
3,949,732	+	ilvG	b4488	0.70	-0.4
3,949,732	+	ilvG	b4488	0.70	+0.7
3,949,789	+	ilvG	b4488	0.73	-0.1
3,949,837	-	ilvG	b4488	0.76	+0.0
3,949,839	-	ilvG	b4488	0.76	+0.6
3,949,839	-	ilvG	b4488	0.76	-0.1
3,949,863	-	ilvG	b4488	0.78	-0.3
3,949,951	+	ilvG	b4488	0.83	+0.0
3,949,959	-	ilvG	b4488	0.84	+1.3
3,950,004	+	ilvG	b4488	0.86	-0.2
3,950,004	+	ilvG	b4488	0.86	+0.3
3,950,063	+				-0.0
3,950,064	+				+0.1
3,950,106	+				+0.1
3,950,109	-				+0.5
3,950,109	-				-1.6
3,950,111	+				+0.2
3,950,119	-				-0.1
3,950,119	-				-0.5
3,950,119	-				+0.1
3,950,121	+				-2.6
3,950,121	+				+0.4
3,950,127	+				-0.4
3,950,140	-				-0.5
3,950,140	-				+0.2
3,950,228	+				-0.4
3,950,251	-	ilvM	b3769	0.10	-0.2
3,950,301	-	ilvM	b3769	0.29	+0.0
3,950,301	-	ilvM	b3769	0.29	+0.2
3,950,381	+	ilvM	b3769	0.59	-0.1
3,950,381	+	ilvM	b3769	0.59	-2.5
3,950,381	+	ilvM	b3769	0.59	-0.1
3,950,381	+	ilvM	b3769	0.59	-0.4
3,950,381	+	ilvM	b3769	0.59	+0.4
3,950,389	-	ilvM	b3769	0.62	-0.4
3,950,456	+	ilvM	b3769	0.88	-0.2
3,950,475	-				-1.1
3,950,475	-				-1.8
3,950,478	-				+0.5
3,950,478	-				-0.3
3,950,592	-				-0.3
3,950,599	-				+0.7
3,950,631	+	ilvE	b3770	0.13	-0.1
3,950,631	+	ilvE	b3770	0.13	+0.3
3,950,654	+	ilvE	b3770	0.16	-1.4
3,950,654	+	ilvE	b3770	0.16	-0.2
3,950,654	+	ilvE	b3770	0.16	-0.0
3,950,675	+	ilvE	b3770	0.18	+1.2
3,950,675	+	ilvE	b3770	0.18	-0.8
3,950,695	-	ilvE	b3770	0.20	+0.0
3,950,713	+	ilvE	b3770	0.22	+0.2
3,950,789	+	ilvE	b3770	0.30	+1.0
3,950,789	+	ilvE	b3770	0.30	-0.1
3,950,789	+	ilvE	b3770	0.30	+0.2
3,950,797	-	ilvE	b3770	0.31	+0.0
3,950,797	-	ilvE	b3770	0.31	-0.4
3,950,797	-	ilvE	b3770	0.31	+0.5
3,950,798	-	ilvE	b3770	0.31	-0.7
3,950,822	-	ilvE	b3770	0.34	+1.2
3,950,822	-	ilvE	b3770	0.34	+0.2
3,950,828	-	ilvE	b3770	0.35	+0.3
3,950,832	+	ilvE	b3770	0.35	-0.0
3,950,851	+	ilvE	b3770	0.37	-0.4
3,950,851	+	ilvE	b3770	0.37	-0.8
3,950,942	+	ilvE	b3770	0.47	+1.0
3,950,948	-	ilvE	b3770	0.47	-0.8
3,950,952	+	ilvE	b3770	0.48	-0.2
3,950,967	-	ilvE	b3770	0.49	+0.5
3,951,009	-	ilvE	b3770	0.54	+3.0
3,951,022	-	ilvE	b3770	0.55	+0.3
3,951,054	+	ilvE	b3770	0.59	-0.9
3,951,057	-	ilvE	b3770	0.59	-0.3
3,951,164	+	ilvE	b3770	0.71	+0.5
3,951,164	+	ilvE	b3770	0.71	-0.7
3,951,168	-	ilvE	b3770	0.71	-0.0
3,951,170	+	ilvE	b3770	0.71	+0.2
3,951,171	-	ilvE	b3770	0.71	+0.3
3,951,242	+	ilvE	b3770	0.79	+0.6
3,951,247	+	ilvE	b3770	0.80	-0.1
3,951,314	-	ilvE	b3770	0.87	+0.7
3,951,315	+	ilvE	b3770	0.87	-0.7
3,951,323	-	ilvE	b3770	0.88	+0.1
3,951,323	-	ilvE	b3770	0.88	+0.5
3,951,334	+	ilvE	b3770	0.89	+0.1
3,951,390	-				+0.1
3,951,461	-				+0.7
3,951,461	-				+0.4
3,951,499	-				+0.2
3,951,512	-				-0.6
3,951,512	-				+0.6
3,951,571	+				+0.6
3,951,571	+				+0.3
3,951,579	-				-0.5
3,951,613	+				+0.7
3,951,634	+				-0.1
3,951,644	+				-0.3
3,951,756	+	ilvD	b3771	0.14	+1.0
3,951,823	-	ilvD	b3771	0.17	+0.5
3,951,824	-	ilvD	b3771	0.17	+0.2
3,951,838	-	ilvD	b3771	0.18	-0.9
3,951,849	-	ilvD	b3771	0.19	+1.1
3,951,857	+	ilvD	b3771	0.19	+0.8
3,951,895	-	ilvD	b3771	0.21	+0.3
3,952,000	+	ilvD	b3771	0.27	-0.5
3,952,005	+	ilvD	b3771	0.27	+0.9
3,952,005	+	ilvD	b3771	0.27	+0.9
3,952,005	+	ilvD	b3771	0.27	+0.1
3,952,017	+	ilvD	b3771	0.28	+0.3
3,952,017	+	ilvD	b3771	0.28	-0.1
3,952,025	-	ilvD	b3771	0.28	+1.3
3,952,025	-	ilvD	b3771	0.28	+0.9
3,952,099	+	ilvD	b3771	0.32	+1.5
3,952,099	+	ilvD	b3771	0.32	-0.1
3,952,196	+	ilvD	b3771	0.38	+0.8
3,952,201	-	ilvD	b3771	0.38	+0.3
3,952,325	+	ilvD	b3771	0.45	+0.0
3,952,340	+	ilvD	b3771	0.45	-0.8
3,952,353	-	ilvD	b3771	0.46	+0.3
3,952,374	-	ilvD	b3771	0.47	+2.3
3,952,374	-	ilvD	b3771	0.47	+1.3
3,952,433	+	ilvD	b3771	0.50	+0.5