Experiment: 3-methyl-3-butenol 0.625 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdnG and fdnH are separated by 12 nucleotides fdnH and fdnI overlap by 8 nucleotides fdnI and yddM are separated by 406 nucleotides yddM and adhP are separated by 145 nucleotides
b1474: fdnG - formate dehydrogenase-N, alpha subunit, nitrate-inducible (NCBI), at 1,545,425 to 1,548,472
fdnG
b1475: fdnH - formate dehydrogenase-N, Fe-S (beta) subunit, nitrate-inducible (NCBI), at 1,548,485 to 1,549,369
fdnH
b1476: fdnI - formate dehydrogenase-N, cytochrome B556 (gamma) subunit, nitrate-inducible (NCBI), at 1,549,362 to 1,550,015
fdnI
b1477: yddM - predicted DNA-binding transcriptional regulator (RefSeq), at 1,550,422 to 1,550,706
yddM
b1478: adhP - alcohol dehydrogenase (VIMSS), at 1,550,852 to 1,551,862
adhP
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1548.444 kb on - strand at 1548.444 kb on - strand at 1548.465 kb on + strand at 1548.607 kb on - strand, within fdnH at 1548.745 kb on - strand, within fdnH at 1548.745 kb on - strand, within fdnH at 1548.789 kb on + strand, within fdnH at 1548.789 kb on + strand, within fdnH at 1549.042 kb on - strand, within fdnH at 1549.104 kb on + strand, within fdnH at 1549.104 kb on + strand, within fdnH at 1549.200 kb on - strand, within fdnH at 1549.396 kb on - strand at 1549.396 kb on - strand at 1549.813 kb on - strand, within fdnI at 1550.056 kb on - strand at 1550.189 kb on - strand at 1550.189 kb on - strand at 1550.209 kb on - strand at 1550.806 kb on + strand at 1550.833 kb on - strand at 1551.011 kb on + strand, within adhP
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol 0.625 vol% remove 1,548,444 - -0.6 1,548,444 - -0.3 1,548,465 + -0.1 1,548,607 - fdnH b1475 0.14 +0.2 1,548,745 - fdnH b1475 0.29 +0.5 1,548,745 - fdnH b1475 0.29 -0.2 1,548,789 + fdnH b1475 0.34 -0.0 1,548,789 + fdnH b1475 0.34 -2.5 1,549,042 - fdnH b1475 0.63 +0.0 1,549,104 + fdnH b1475 0.70 -0.1 1,549,104 + fdnH b1475 0.70 -0.2 1,549,200 - fdnH b1475 0.81 +2.0 1,549,396 - -0.0 1,549,396 - +0.7 1,549,813 - fdnI b1476 0.69 -0.2 1,550,056 - +0.1 1,550,189 - +0.3 1,550,189 - -1.0 1,550,209 - +0.2 1,550,806 + -0.2 1,550,833 - -0.2 1,551,011 + adhP b1478 0.16 +0.4
Or see this region's nucleotide sequence