Experiment: 3-methyl-3-butenol 0.625 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybfF and seqA are separated by 184 nucleotides seqA and pgm are separated by 25 nucleotides pgm and ybfP are separated by 213 nucleotides ybfP and ybfG are separated by 40 nucleotides
b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025
ybfF
b0687: seqA - regulatory protein for replication initiation (NCBI), at 712,210 to 712,755
seqA
b0688: pgm - phosphoglucomutase (NCBI), at 712,781 to 714,421
pgm
b0689: ybfP - hypothetical protein (NCBI), at 714,635 to 715,129
ybfP
b0691: ybfG - orf, hypothetical protein (VIMSS), at 715,170 to 715,820
ybfG
Position (kb) 712
713
714
715 Strain fitness (log2 ratio) -2
-1
0
1 at 711.803 kb on - strand, within ybfF at 711.803 kb on - strand, within ybfF at 711.900 kb on - strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.933 kb on + strand, within ybfF at 712.060 kb on + strand at 712.077 kb on + strand at 712.124 kb on + strand at 712.202 kb on + strand at 712.758 kb on - strand at 712.814 kb on - strand at 712.937 kb on + strand at 712.978 kb on + strand, within pgm at 713.023 kb on + strand, within pgm at 713.081 kb on + strand, within pgm at 713.205 kb on + strand, within pgm at 713.428 kb on - strand, within pgm at 713.435 kb on + strand, within pgm at 713.435 kb on + strand, within pgm at 713.525 kb on - strand, within pgm at 713.580 kb on + strand, within pgm at 713.655 kb on - strand, within pgm at 713.777 kb on + strand, within pgm at 713.784 kb on - strand, within pgm at 713.863 kb on - strand, within pgm at 713.915 kb on + strand, within pgm at 713.955 kb on + strand, within pgm at 714.076 kb on + strand, within pgm at 714.206 kb on + strand, within pgm at 714.260 kb on + strand at 714.260 kb on + strand at 714.309 kb on - strand at 714.599 kb on + strand at 714.653 kb on - strand at 714.655 kb on + strand at 714.659 kb on + strand at 714.659 kb on + strand at 714.667 kb on - strand at 714.689 kb on - strand, within ybfP at 714.689 kb on - strand, within ybfP at 714.691 kb on + strand, within ybfP at 714.769 kb on - strand, within ybfP at 714.769 kb on - strand, within ybfP at 714.842 kb on + strand, within ybfP at 714.842 kb on + strand, within ybfP at 714.864 kb on + strand, within ybfP at 714.884 kb on + strand, within ybfP at 714.884 kb on + strand, within ybfP at 714.949 kb on - strand, within ybfP at 714.949 kb on - strand, within ybfP at 714.951 kb on - strand, within ybfP at 714.951 kb on - strand, within ybfP at 714.952 kb on - strand, within ybfP at 714.974 kb on + strand, within ybfP at 714.979 kb on + strand, within ybfP at 714.986 kb on + strand, within ybfP at 714.986 kb on + strand, within ybfP at 714.986 kb on + strand, within ybfP at 714.986 kb on + strand, within ybfP at 714.987 kb on - strand, within ybfP at 714.987 kb on - strand, within ybfP at 714.993 kb on - strand, within ybfP at 714.994 kb on - strand, within ybfP at 714.997 kb on + strand, within ybfP at 714.997 kb on + strand, within ybfP at 715.019 kb on + strand, within ybfP at 715.052 kb on - strand, within ybfP at 715.067 kb on + strand, within ybfP at 715.068 kb on + strand, within ybfP at 715.158 kb on - strand at 715.159 kb on - strand at 715.159 kb on - strand at 715.169 kb on - strand at 715.169 kb on - strand at 715.175 kb on - strand at 715.175 kb on - strand at 715.207 kb on + strand at 715.262 kb on - strand, within ybfG at 715.262 kb on - strand, within ybfG at 715.264 kb on + strand, within ybfG at 715.272 kb on + strand, within ybfG at 715.272 kb on - strand, within ybfG at 715.272 kb on - strand, within ybfG at 715.287 kb on - strand, within ybfG at 715.305 kb on - strand, within ybfG at 715.305 kb on - strand, within ybfG
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-3-butenol 0.625 vol% remove 711,803 - ybfF b0686 0.71 -0.6 711,803 - ybfF b0686 0.71 -0.3 711,900 - ybfF b0686 0.84 +0.2 711,923 + ybfF b0686 0.87 -0.3 711,923 + ybfF b0686 0.87 -0.4 711,933 + ybfF b0686 0.88 -0.3 712,060 + +0.4 712,077 + -0.8 712,124 + +1.2 712,202 + +1.0 712,758 - +0.1 712,814 - -1.2 712,937 + -2.5 712,978 + pgm b0688 0.12 -0.5 713,023 + pgm b0688 0.15 +0.3 713,081 + pgm b0688 0.18 +1.0 713,205 + pgm b0688 0.26 +0.1 713,428 - pgm b0688 0.39 -0.0 713,435 + pgm b0688 0.40 +0.5 713,435 + pgm b0688 0.40 +1.3 713,525 - pgm b0688 0.45 -0.3 713,580 + pgm b0688 0.49 -0.2 713,655 - pgm b0688 0.53 -0.2 713,777 + pgm b0688 0.61 -0.5 713,784 - pgm b0688 0.61 +0.5 713,863 - pgm b0688 0.66 -0.0 713,915 + pgm b0688 0.69 -1.0 713,955 + pgm b0688 0.72 -0.0 714,076 + pgm b0688 0.79 -1.2 714,206 + pgm b0688 0.87 -0.2 714,260 + -0.8 714,260 + -0.0 714,309 - -0.5 714,599 + -0.0 714,653 - -0.0 714,655 + +0.2 714,659 + -0.5 714,659 + -0.3 714,667 - -0.2 714,689 - ybfP b0689 0.11 -0.0 714,689 - ybfP b0689 0.11 +0.6 714,691 + ybfP b0689 0.11 +0.6 714,769 - ybfP b0689 0.27 +1.6 714,769 - ybfP b0689 0.27 -0.0 714,842 + ybfP b0689 0.42 -0.1 714,842 + ybfP b0689 0.42 +0.1 714,864 + ybfP b0689 0.46 +0.5 714,884 + ybfP b0689 0.50 -0.4 714,884 + ybfP b0689 0.50 +0.2 714,949 - ybfP b0689 0.63 +0.5 714,949 - ybfP b0689 0.63 -0.0 714,951 - ybfP b0689 0.64 +1.0 714,951 - ybfP b0689 0.64 -0.8 714,952 - ybfP b0689 0.64 +0.4 714,974 + ybfP b0689 0.68 +0.4 714,979 + ybfP b0689 0.69 -0.4 714,986 + ybfP b0689 0.71 +0.5 714,986 + ybfP b0689 0.71 -0.2 714,986 + ybfP b0689 0.71 +0.4 714,986 + ybfP b0689 0.71 -0.0 714,987 - ybfP b0689 0.71 +0.2 714,987 - ybfP b0689 0.71 +0.9 714,993 - ybfP b0689 0.72 -0.4 714,994 - ybfP b0689 0.73 -0.4 714,997 + ybfP b0689 0.73 -0.3 714,997 + ybfP b0689 0.73 +0.0 715,019 + ybfP b0689 0.78 -0.2 715,052 - ybfP b0689 0.84 +0.0 715,067 + ybfP b0689 0.87 +0.1 715,068 + ybfP b0689 0.87 -1.0 715,158 - +0.5 715,159 - -1.0 715,159 - +1.4 715,169 - -0.5 715,169 - -0.3 715,175 - -0.7 715,175 - -0.7 715,207 + +0.9 715,262 - ybfG b0691 0.14 -1.3 715,262 - ybfG b0691 0.14 +0.7 715,264 + ybfG b0691 0.14 -0.3 715,272 + ybfG b0691 0.16 +0.3 715,272 - ybfG b0691 0.16 +1.0 715,272 - ybfG b0691 0.16 -0.8 715,287 - ybfG b0691 0.18 -0.5 715,305 - ybfG b0691 0.21 -1.0 715,305 - ybfG b0691 0.21 +1.0
Or see this region's nucleotide sequence