Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_2352

Experiment: KB with Rotenone 0.25 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2350 and Psyr_2351 are separated by 78 nucleotidesPsyr_2351 and Psyr_2352 are separated by 289 nucleotidesPsyr_2352 and Psyr_2353 are separated by 115 nucleotides Psyr_2350: Psyr_2350 - Protein of unknown function UPF0025, at 2,726,540 to 2,726,998 _2350 Psyr_2351: Psyr_2351 - tRNA synthetase, class-II (G, H, P and S), at 2,727,077 to 2,727,658 _2351 Psyr_2352: Psyr_2352 - GCN5-related N-acetyltransferase, at 2,727,948 to 2,728,475 _2352 Psyr_2353: Psyr_2353 - Uncharacterized protein conserved in bacteria-like protein, at 2,728,591 to 2,729,817 _2353 Position (kb) 2727 2728 2729Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2726.999 kb on - strandat 2727.038 kb on + strandat 2727.054 kb on + strandat 2727.055 kb on - strandat 2727.064 kb on + strandat 2727.220 kb on - strand, within Psyr_2351at 2727.222 kb on + strand, within Psyr_2351at 2727.222 kb on + strand, within Psyr_2351at 2727.222 kb on + strand, within Psyr_2351at 2727.223 kb on - strand, within Psyr_2351at 2727.223 kb on - strand, within Psyr_2351at 2727.258 kb on + strand, within Psyr_2351at 2727.414 kb on + strand, within Psyr_2351at 2727.631 kb on + strandat 2727.632 kb on - strandat 2727.632 kb on - strandat 2727.632 kb on - strandat 2727.927 kb on - strandat 2728.003 kb on + strand, within Psyr_2352at 2728.046 kb on - strand, within Psyr_2352at 2728.075 kb on + strand, within Psyr_2352at 2728.075 kb on + strand, within Psyr_2352at 2728.076 kb on - strand, within Psyr_2352at 2728.378 kb on + strand, within Psyr_2352at 2728.378 kb on + strand, within Psyr_2352at 2728.378 kb on + strand, within Psyr_2352at 2728.378 kb on + strand, within Psyr_2352at 2728.378 kb on + strand, within Psyr_2352at 2728.378 kb on + strand, within Psyr_2352at 2728.379 kb on - strand, within Psyr_2352at 2728.379 kb on - strand, within Psyr_2352at 2728.379 kb on - strand, within Psyr_2352at 2728.379 kb on - strand, within Psyr_2352at 2728.530 kb on + strandat 2728.530 kb on + strandat 2728.530 kb on + strandat 2728.530 kb on + strandat 2728.530 kb on + strandat 2728.531 kb on - strandat 2728.565 kb on + strandat 2728.565 kb on + strandat 2728.565 kb on + strandat 2728.566 kb on - strandat 2728.570 kb on + strandat 2728.571 kb on - strandat 2728.574 kb on + strandat 2728.920 kb on - strand, within Psyr_2353at 2728.920 kb on - strand, within Psyr_2353at 2728.935 kb on - strand, within Psyr_2353at 2728.987 kb on + strand, within Psyr_2353at 2728.987 kb on + strand, within Psyr_2353at 2728.987 kb on + strand, within Psyr_2353at 2728.987 kb on + strand, within Psyr_2353at 2728.988 kb on - strand, within Psyr_2353at 2728.988 kb on - strand, within Psyr_2353at 2728.988 kb on - strand, within Psyr_2353at 2728.988 kb on - strand, within Psyr_2353at 2728.991 kb on + strand, within Psyr_2353at 2728.991 kb on + strand, within Psyr_2353at 2728.992 kb on - strand, within Psyr_2353at 2728.992 kb on - strand, within Psyr_2353at 2728.992 kb on - strand, within Psyr_2353at 2728.992 kb on - strand, within Psyr_2353at 2728.992 kb on - strand, within Psyr_2353at 2729.287 kb on + strand, within Psyr_2353at 2729.288 kb on - strand, within Psyr_2353at 2729.335 kb on - strand, within Psyr_2353at 2729.362 kb on + strand, within Psyr_2353at 2729.362 kb on + strand, within Psyr_2353at 2729.362 kb on + strand, within Psyr_2353at 2729.362 kb on + strand, within Psyr_2353at 2729.362 kb on + strand, within Psyr_2353at 2729.362 kb on + strand, within Psyr_2353at 2729.363 kb on - strand, within Psyr_2353at 2729.363 kb on - strand, within Psyr_2353at 2729.363 kb on - strand, within Psyr_2353at 2729.363 kb on - strand, within Psyr_2353at 2729.408 kb on + strand, within Psyr_2353at 2729.408 kb on + strand, within Psyr_2353at 2729.409 kb on - strand, within Psyr_2353

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml
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2,726,999 - -0.2
2,727,038 + +1.4
2,727,054 + +0.5
2,727,055 - -1.0
2,727,064 + +2.2
2,727,220 - Psyr_2351 0.25 +0.9
2,727,222 + Psyr_2351 0.25 +0.0
2,727,222 + Psyr_2351 0.25 -1.6
2,727,222 + Psyr_2351 0.25 -0.0
2,727,223 - Psyr_2351 0.25 -0.6
2,727,223 - Psyr_2351 0.25 +1.1
2,727,258 + Psyr_2351 0.31 -0.9
2,727,414 + Psyr_2351 0.58 +0.0
2,727,631 + +0.8
2,727,632 - +0.0
2,727,632 - -0.2
2,727,632 - -0.2
2,727,927 - +0.6
2,728,003 + Psyr_2352 0.10 +1.5
2,728,046 - Psyr_2352 0.19 -1.9
2,728,075 + Psyr_2352 0.24 -1.6
2,728,075 + Psyr_2352 0.24 -0.1
2,728,076 - Psyr_2352 0.24 -1.4
2,728,378 + Psyr_2352 0.81 -0.2
2,728,378 + Psyr_2352 0.81 -0.8
2,728,378 + Psyr_2352 0.81 -3.4
2,728,378 + Psyr_2352 0.81 -0.0
2,728,378 + Psyr_2352 0.81 +0.1
2,728,378 + Psyr_2352 0.81 -0.4
2,728,379 - Psyr_2352 0.82 -0.3
2,728,379 - Psyr_2352 0.82 -0.1
2,728,379 - Psyr_2352 0.82 +0.2
2,728,379 - Psyr_2352 0.82 -0.4
2,728,530 + -0.2
2,728,530 + +0.3
2,728,530 + -0.1
2,728,530 + -0.4
2,728,530 + +0.9
2,728,531 - -0.5
2,728,565 + -0.6
2,728,565 + -0.4
2,728,565 + -0.5
2,728,566 - -0.4
2,728,570 + -0.3
2,728,571 - -1.3
2,728,574 + -0.2
2,728,920 - Psyr_2353 0.27 +0.8
2,728,920 - Psyr_2353 0.27 -1.4
2,728,935 - Psyr_2353 0.28 -0.7
2,728,987 + Psyr_2353 0.32 -0.7
2,728,987 + Psyr_2353 0.32 -1.0
2,728,987 + Psyr_2353 0.32 +0.8
2,728,987 + Psyr_2353 0.32 +0.1
2,728,988 - Psyr_2353 0.32 -0.7
2,728,988 - Psyr_2353 0.32 +1.0
2,728,988 - Psyr_2353 0.32 -0.4
2,728,988 - Psyr_2353 0.32 +2.0
2,728,991 + Psyr_2353 0.33 +0.7
2,728,991 + Psyr_2353 0.33 +1.7
2,728,992 - Psyr_2353 0.33 -2.6
2,728,992 - Psyr_2353 0.33 -0.4
2,728,992 - Psyr_2353 0.33 -1.0
2,728,992 - Psyr_2353 0.33 -1.3
2,728,992 - Psyr_2353 0.33 -0.0
2,729,287 + Psyr_2353 0.57 -0.3
2,729,288 - Psyr_2353 0.57 -2.2
2,729,335 - Psyr_2353 0.61 -0.4
2,729,362 + Psyr_2353 0.63 +1.0
2,729,362 + Psyr_2353 0.63 +0.1
2,729,362 + Psyr_2353 0.63 +0.7
2,729,362 + Psyr_2353 0.63 +0.0
2,729,362 + Psyr_2353 0.63 -0.2
2,729,362 + Psyr_2353 0.63 +0.2
2,729,363 - Psyr_2353 0.63 -1.9
2,729,363 - Psyr_2353 0.63 -1.3
2,729,363 - Psyr_2353 0.63 -0.7
2,729,363 - Psyr_2353 0.63 -0.3
2,729,408 + Psyr_2353 0.67 +0.4
2,729,408 + Psyr_2353 0.67 +0.1
2,729,409 - Psyr_2353 0.67 -0.1

Or see this region's nucleotide sequence