Experiment: KB with Rotenone 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2350 and Psyr_2351 are separated by 78 nucleotides Psyr_2351 and Psyr_2352 are separated by 289 nucleotides Psyr_2352 and Psyr_2353 are separated by 115 nucleotides
Psyr_2350: Psyr_2350 - Protein of unknown function UPF0025, at 2,726,540 to 2,726,998
_2350
Psyr_2351: Psyr_2351 - tRNA synthetase, class-II (G, H, P and S), at 2,727,077 to 2,727,658
_2351
Psyr_2352: Psyr_2352 - GCN5-related N-acetyltransferase, at 2,727,948 to 2,728,475
_2352
Psyr_2353: Psyr_2353 - Uncharacterized protein conserved in bacteria-like protein, at 2,728,591 to 2,729,817
_2353
Position (kb)
2727
2728
2729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2726.999 kb on - strand at 2727.038 kb on + strand at 2727.054 kb on + strand at 2727.055 kb on - strand at 2727.064 kb on + strand at 2727.220 kb on - strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.222 kb on + strand, within Psyr_2351 at 2727.223 kb on - strand, within Psyr_2351 at 2727.223 kb on - strand, within Psyr_2351 at 2727.258 kb on + strand, within Psyr_2351 at 2727.414 kb on + strand, within Psyr_2351 at 2727.631 kb on + strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.632 kb on - strand at 2727.927 kb on - strand at 2728.003 kb on + strand, within Psyr_2352 at 2728.046 kb on - strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.075 kb on + strand, within Psyr_2352 at 2728.076 kb on - strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.378 kb on + strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.379 kb on - strand, within Psyr_2352 at 2728.530 kb on + strand at 2728.530 kb on + strand at 2728.530 kb on + strand at 2728.530 kb on + strand at 2728.530 kb on + strand at 2728.531 kb on - strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.565 kb on + strand at 2728.566 kb on - strand at 2728.570 kb on + strand at 2728.571 kb on - strand at 2728.574 kb on + strand at 2728.920 kb on - strand, within Psyr_2353 at 2728.920 kb on - strand, within Psyr_2353 at 2728.935 kb on - strand, within Psyr_2353 at 2728.987 kb on + strand, within Psyr_2353 at 2728.987 kb on + strand, within Psyr_2353 at 2728.987 kb on + strand, within Psyr_2353 at 2728.987 kb on + strand, within Psyr_2353 at 2728.988 kb on - strand, within Psyr_2353 at 2728.988 kb on - strand, within Psyr_2353 at 2728.988 kb on - strand, within Psyr_2353 at 2728.988 kb on - strand, within Psyr_2353 at 2728.991 kb on + strand, within Psyr_2353 at 2728.991 kb on + strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2728.992 kb on - strand, within Psyr_2353 at 2729.287 kb on + strand, within Psyr_2353 at 2729.288 kb on - strand, within Psyr_2353 at 2729.335 kb on - strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.362 kb on + strand, within Psyr_2353 at 2729.363 kb on - strand, within Psyr_2353 at 2729.363 kb on - strand, within Psyr_2353 at 2729.363 kb on - strand, within Psyr_2353 at 2729.363 kb on - strand, within Psyr_2353 at 2729.408 kb on + strand, within Psyr_2353 at 2729.408 kb on + strand, within Psyr_2353 at 2729.409 kb on - strand, within Psyr_2353
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Rotenone 0.25 mg/ml remove 2,726,999 - -0.2 2,727,038 + +1.4 2,727,054 + +0.5 2,727,055 - -1.0 2,727,064 + +2.2 2,727,220 - Psyr_2351 0.25 +0.9 2,727,222 + Psyr_2351 0.25 +0.0 2,727,222 + Psyr_2351 0.25 -1.6 2,727,222 + Psyr_2351 0.25 -0.0 2,727,223 - Psyr_2351 0.25 -0.6 2,727,223 - Psyr_2351 0.25 +1.1 2,727,258 + Psyr_2351 0.31 -0.9 2,727,414 + Psyr_2351 0.58 +0.0 2,727,631 + +0.8 2,727,632 - +0.0 2,727,632 - -0.2 2,727,632 - -0.2 2,727,927 - +0.6 2,728,003 + Psyr_2352 0.10 +1.5 2,728,046 - Psyr_2352 0.19 -1.9 2,728,075 + Psyr_2352 0.24 -1.6 2,728,075 + Psyr_2352 0.24 -0.1 2,728,076 - Psyr_2352 0.24 -1.4 2,728,378 + Psyr_2352 0.81 -0.2 2,728,378 + Psyr_2352 0.81 -0.8 2,728,378 + Psyr_2352 0.81 -3.4 2,728,378 + Psyr_2352 0.81 -0.0 2,728,378 + Psyr_2352 0.81 +0.1 2,728,378 + Psyr_2352 0.81 -0.4 2,728,379 - Psyr_2352 0.82 -0.3 2,728,379 - Psyr_2352 0.82 -0.1 2,728,379 - Psyr_2352 0.82 +0.2 2,728,379 - Psyr_2352 0.82 -0.4 2,728,530 + -0.2 2,728,530 + +0.3 2,728,530 + -0.1 2,728,530 + -0.4 2,728,530 + +0.9 2,728,531 - -0.5 2,728,565 + -0.6 2,728,565 + -0.4 2,728,565 + -0.5 2,728,566 - -0.4 2,728,570 + -0.3 2,728,571 - -1.3 2,728,574 + -0.2 2,728,920 - Psyr_2353 0.27 +0.8 2,728,920 - Psyr_2353 0.27 -1.4 2,728,935 - Psyr_2353 0.28 -0.7 2,728,987 + Psyr_2353 0.32 -0.7 2,728,987 + Psyr_2353 0.32 -1.0 2,728,987 + Psyr_2353 0.32 +0.8 2,728,987 + Psyr_2353 0.32 +0.1 2,728,988 - Psyr_2353 0.32 -0.7 2,728,988 - Psyr_2353 0.32 +1.0 2,728,988 - Psyr_2353 0.32 -0.4 2,728,988 - Psyr_2353 0.32 +2.0 2,728,991 + Psyr_2353 0.33 +0.7 2,728,991 + Psyr_2353 0.33 +1.7 2,728,992 - Psyr_2353 0.33 -2.6 2,728,992 - Psyr_2353 0.33 -0.4 2,728,992 - Psyr_2353 0.33 -1.0 2,728,992 - Psyr_2353 0.33 -1.3 2,728,992 - Psyr_2353 0.33 -0.0 2,729,287 + Psyr_2353 0.57 -0.3 2,729,288 - Psyr_2353 0.57 -2.2 2,729,335 - Psyr_2353 0.61 -0.4 2,729,362 + Psyr_2353 0.63 +1.0 2,729,362 + Psyr_2353 0.63 +0.1 2,729,362 + Psyr_2353 0.63 +0.7 2,729,362 + Psyr_2353 0.63 +0.0 2,729,362 + Psyr_2353 0.63 -0.2 2,729,362 + Psyr_2353 0.63 +0.2 2,729,363 - Psyr_2353 0.63 -1.9 2,729,363 - Psyr_2353 0.63 -1.3 2,729,363 - Psyr_2353 0.63 -0.7 2,729,363 - Psyr_2353 0.63 -0.3 2,729,408 + Psyr_2353 0.67 +0.4 2,729,408 + Psyr_2353 0.67 +0.1 2,729,409 - Psyr_2353 0.67 -0.1
Or see this region's nucleotide sequence