Experiment: R2A with nitrite 9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5423 and Atu5424 are separated by 60 nucleotides Atu5424 and Atu5425 are separated by 10 nucleotides Atu5425 and Atu5426 are separated by 7 nucleotides
Atu5423: Atu5423 - ABC transporter nucleotide binding/ATPase (putrescine), at 417,753 to 418,823
Atu5423
Atu5424: Atu5424 - oxidoreductase, at 418,884 to 420,362
Atu5424
Atu5425: Atu5425 - 2-hydroxychromene-2-carboxylate isomerase, at 420,373 to 420,966
Atu5425
Atu5426: Atu5426 - hypothetical protein, at 420,974 to 421,480
Atu5426
Position (kb) 418
419
420
421 Strain fitness (log2 ratio) -1
0
1
2 at 417.899 kb on + strand, within Atu5423 at 417.976 kb on + strand, within Atu5423 at 417.976 kb on + strand, within Atu5423 at 418.032 kb on - strand, within Atu5423 at 418.088 kb on + strand, within Atu5423 at 418.160 kb on - strand, within Atu5423 at 418.187 kb on + strand, within Atu5423 at 418.249 kb on + strand, within Atu5423 at 418.250 kb on - strand, within Atu5423 at 418.397 kb on + strand, within Atu5423 at 418.400 kb on - strand, within Atu5423 at 418.410 kb on + strand, within Atu5423 at 418.507 kb on - strand, within Atu5423 at 418.567 kb on - strand, within Atu5423 at 418.567 kb on - strand, within Atu5423 at 418.595 kb on + strand, within Atu5423 at 418.603 kb on - strand, within Atu5423 at 418.613 kb on - strand, within Atu5423 at 418.673 kb on + strand, within Atu5423 at 418.673 kb on + strand, within Atu5423 at 418.681 kb on - strand, within Atu5423 at 418.784 kb on + strand at 418.804 kb on + strand at 418.804 kb on + strand at 418.817 kb on - strand at 418.838 kb on + strand at 418.904 kb on - strand at 418.904 kb on - strand at 418.911 kb on + strand at 418.940 kb on + strand at 418.943 kb on - strand at 418.948 kb on - strand at 418.974 kb on + strand at 418.977 kb on - strand at 418.984 kb on + strand at 418.984 kb on + strand at 418.994 kb on + strand at 418.994 kb on + strand at 418.994 kb on + strand at 419.167 kb on - strand, within Atu5424 at 419.178 kb on + strand, within Atu5424 at 419.194 kb on + strand, within Atu5424 at 419.288 kb on + strand, within Atu5424 at 419.296 kb on - strand, within Atu5424 at 419.315 kb on + strand, within Atu5424 at 419.459 kb on + strand, within Atu5424 at 419.474 kb on - strand, within Atu5424 at 419.501 kb on + strand, within Atu5424 at 419.587 kb on + strand, within Atu5424 at 419.589 kb on + strand, within Atu5424 at 419.606 kb on + strand, within Atu5424 at 419.671 kb on - strand, within Atu5424 at 419.773 kb on - strand, within Atu5424 at 419.793 kb on + strand, within Atu5424 at 419.801 kb on + strand, within Atu5424 at 419.803 kb on + strand, within Atu5424 at 419.997 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.020 kb on + strand, within Atu5424 at 420.028 kb on - strand, within Atu5424 at 420.032 kb on + strand, within Atu5424 at 420.032 kb on + strand, within Atu5424 at 420.050 kb on + strand, within Atu5424 at 420.129 kb on + strand, within Atu5424 at 420.220 kb on - strand at 420.221 kb on + strand at 420.266 kb on + strand at 420.274 kb on - strand at 420.277 kb on + strand at 420.318 kb on - strand at 420.318 kb on - strand at 420.318 kb on - strand at 420.331 kb on + strand at 420.347 kb on + strand at 420.365 kb on - strand at 420.371 kb on - strand at 420.423 kb on - strand at 420.427 kb on - strand at 420.449 kb on - strand, within Atu5425 at 420.475 kb on - strand, within Atu5425 at 420.535 kb on + strand, within Atu5425 at 420.549 kb on + strand, within Atu5425 at 420.675 kb on + strand, within Atu5425 at 420.683 kb on - strand, within Atu5425 at 420.686 kb on + strand, within Atu5425 at 420.706 kb on - strand, within Atu5425 at 420.730 kb on - strand, within Atu5425 at 420.743 kb on - strand, within Atu5425 at 420.799 kb on - strand, within Atu5425 at 420.799 kb on - strand, within Atu5425 at 420.816 kb on - strand, within Atu5425 at 420.841 kb on + strand, within Atu5425 at 420.852 kb on + strand, within Atu5425 at 420.919 kb on + strand at 420.922 kb on - strand at 420.927 kb on - strand at 420.986 kb on + strand at 421.025 kb on - strand, within Atu5426 at 421.029 kb on + strand, within Atu5426 at 421.074 kb on - strand, within Atu5426 at 421.118 kb on - strand, within Atu5426 at 421.143 kb on + strand, within Atu5426 at 421.143 kb on + strand, within Atu5426 at 421.201 kb on - strand, within Atu5426 at 421.222 kb on + strand, within Atu5426 at 421.222 kb on + strand, within Atu5426
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 9 mM remove 417,899 + Atu5423 0.14 +1.1 417,976 + Atu5423 0.21 +1.5 417,976 + Atu5423 0.21 -0.9 418,032 - Atu5423 0.26 +0.6 418,088 + Atu5423 0.31 +0.9 418,160 - Atu5423 0.38 -0.2 418,187 + Atu5423 0.41 +0.3 418,249 + Atu5423 0.46 -0.3 418,250 - Atu5423 0.46 +1.3 418,397 + Atu5423 0.60 +0.3 418,400 - Atu5423 0.60 +0.3 418,410 + Atu5423 0.61 +0.4 418,507 - Atu5423 0.70 -0.3 418,567 - Atu5423 0.76 -0.6 418,567 - Atu5423 0.76 -1.3 418,595 + Atu5423 0.79 +0.9 418,603 - Atu5423 0.79 -0.5 418,613 - Atu5423 0.80 +0.1 418,673 + Atu5423 0.86 +0.1 418,673 + Atu5423 0.86 -0.1 418,681 - Atu5423 0.87 -1.5 418,784 + +0.2 418,804 + -0.9 418,804 + -0.5 418,817 - -0.3 418,838 + -0.5 418,904 - +0.7 418,904 - -1.7 418,911 + -0.5 418,940 + -0.8 418,943 - +0.4 418,948 - -0.4 418,974 + -0.3 418,977 - -0.9 418,984 + -0.9 418,984 + +0.1 418,994 + +0.2 418,994 + -0.2 418,994 + +0.4 419,167 - Atu5424 0.19 -0.9 419,178 + Atu5424 0.20 -1.2 419,194 + Atu5424 0.21 -0.7 419,288 + Atu5424 0.27 -0.5 419,296 - Atu5424 0.28 +0.7 419,315 + Atu5424 0.29 -1.7 419,459 + Atu5424 0.39 -0.2 419,474 - Atu5424 0.40 -0.6 419,501 + Atu5424 0.42 +0.4 419,587 + Atu5424 0.48 -0.9 419,589 + Atu5424 0.48 -0.4 419,606 + Atu5424 0.49 -0.3 419,671 - Atu5424 0.53 -1.2 419,773 - Atu5424 0.60 -0.7 419,793 + Atu5424 0.61 -1.0 419,801 + Atu5424 0.62 -0.5 419,803 + Atu5424 0.62 -1.2 419,997 + Atu5424 0.75 +0.0 420,020 + Atu5424 0.77 -0.8 420,020 + Atu5424 0.77 -0.4 420,020 + Atu5424 0.77 -0.7 420,020 + Atu5424 0.77 -0.6 420,020 + Atu5424 0.77 -1.0 420,020 + Atu5424 0.77 -1.2 420,028 - Atu5424 0.77 -1.0 420,032 + Atu5424 0.78 -0.6 420,032 + Atu5424 0.78 -0.4 420,050 + Atu5424 0.79 -1.4 420,129 + Atu5424 0.84 -0.6 420,220 - -0.7 420,221 + +0.6 420,266 + -0.2 420,274 - -0.7 420,277 + +2.3 420,318 - -0.0 420,318 - -0.3 420,318 - +0.4 420,331 + +0.8 420,347 + +0.5 420,365 - +0.1 420,371 - +1.1 420,423 - +0.0 420,427 - -0.7 420,449 - Atu5425 0.13 +0.2 420,475 - Atu5425 0.17 +0.4 420,535 + Atu5425 0.27 -0.3 420,549 + Atu5425 0.30 +1.4 420,675 + Atu5425 0.51 -1.1 420,683 - Atu5425 0.52 +0.6 420,686 + Atu5425 0.53 -0.1 420,706 - Atu5425 0.56 +0.3 420,730 - Atu5425 0.60 +0.5 420,743 - Atu5425 0.62 +0.1 420,799 - Atu5425 0.72 -0.7 420,799 - Atu5425 0.72 +0.3 420,816 - Atu5425 0.75 -0.4 420,841 + Atu5425 0.79 +0.3 420,852 + Atu5425 0.81 +1.3 420,919 + +0.3 420,922 - +0.3 420,927 - +0.4 420,986 + +0.2 421,025 - Atu5426 0.10 +0.1 421,029 + Atu5426 0.11 -0.0 421,074 - Atu5426 0.20 -0.6 421,118 - Atu5426 0.28 -0.9 421,143 + Atu5426 0.33 -0.3 421,143 + Atu5426 0.33 +0.4 421,201 - Atu5426 0.45 -1.2 421,222 + Atu5426 0.49 -0.2 421,222 + Atu5426 0.49 -0.3
Or see this region's nucleotide sequence