Experiment: R2A with nitrite 9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4651 and Atu4652 are separated by 148 nucleotides Atu4652 and Atu4653 overlap by 4 nucleotides Atu4653 and Atu4654 are separated by 17 nucleotides
Atu4651: Atu4651 - argininosuccinate lyase, at 1,804,754 to 1,806,124
Atu4651
Atu4652: Atu4652 - sugar ABC transporter permease, at 1,806,273 to 1,807,115
Atu4652
Atu4653: Atu4653 - sugar ABC transporter permease, at 1,807,112 to 1,808,053
Atu4653
Atu4654: Atu4654 - sugar ABC transporter substrate-binding protein, at 1,808,071 to 1,809,336
Atu4654
Position (kb)
1806
1807
1808 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1805.334 kb on - strand, within Atu4651 at 1805.345 kb on - strand, within Atu4651 at 1805.397 kb on - strand, within Atu4651 at 1805.629 kb on - strand, within Atu4651 at 1805.647 kb on - strand, within Atu4651 at 1805.648 kb on + strand, within Atu4651 at 1805.648 kb on + strand, within Atu4651 at 1805.765 kb on + strand, within Atu4651 at 1805.768 kb on - strand, within Atu4651 at 1805.846 kb on - strand, within Atu4651 at 1805.877 kb on + strand, within Atu4651 at 1805.895 kb on + strand, within Atu4651 at 1805.903 kb on - strand, within Atu4651 at 1805.937 kb on + strand, within Atu4651 at 1805.937 kb on + strand, within Atu4651 at 1805.945 kb on - strand, within Atu4651 at 1805.999 kb on + strand at 1806.028 kb on - strand at 1806.042 kb on + strand at 1806.125 kb on + strand at 1806.130 kb on + strand at 1806.133 kb on - strand at 1806.155 kb on + strand at 1806.265 kb on - strand at 1806.293 kb on + strand at 1806.341 kb on + strand at 1806.349 kb on - strand at 1806.411 kb on + strand, within Atu4652 at 1806.432 kb on + strand, within Atu4652 at 1806.433 kb on + strand, within Atu4652 at 1806.433 kb on + strand, within Atu4652 at 1806.441 kb on - strand, within Atu4652 at 1806.441 kb on - strand, within Atu4652 at 1806.471 kb on + strand, within Atu4652 at 1806.479 kb on - strand, within Atu4652 at 1806.479 kb on - strand, within Atu4652 at 1806.479 kb on - strand, within Atu4652 at 1806.506 kb on - strand, within Atu4652 at 1806.556 kb on - strand, within Atu4652 at 1806.592 kb on + strand, within Atu4652 at 1806.726 kb on - strand, within Atu4652 at 1806.727 kb on + strand, within Atu4652 at 1806.815 kb on + strand, within Atu4652 at 1806.815 kb on + strand, within Atu4652 at 1806.815 kb on + strand, within Atu4652 at 1806.823 kb on - strand, within Atu4652 at 1806.823 kb on - strand, within Atu4652 at 1806.858 kb on + strand, within Atu4652 at 1806.875 kb on + strand, within Atu4652 at 1806.902 kb on - strand, within Atu4652 at 1806.907 kb on - strand, within Atu4652 at 1806.958 kb on + strand, within Atu4652 at 1806.975 kb on - strand, within Atu4652 at 1807.034 kb on + strand at 1807.044 kb on + strand at 1807.131 kb on + strand at 1807.139 kb on - strand at 1807.139 kb on - strand at 1807.194 kb on - strand at 1807.224 kb on + strand, within Atu4653 at 1807.293 kb on + strand, within Atu4653 at 1807.293 kb on + strand, within Atu4653 at 1807.293 kb on + strand, within Atu4653 at 1807.293 kb on + strand, within Atu4653 at 1807.301 kb on - strand, within Atu4653 at 1807.359 kb on + strand, within Atu4653 at 1807.398 kb on - strand, within Atu4653 at 1807.518 kb on + strand, within Atu4653 at 1807.539 kb on - strand, within Atu4653 at 1807.552 kb on - strand, within Atu4653 at 1807.592 kb on - strand, within Atu4653 at 1807.692 kb on + strand, within Atu4653 at 1807.706 kb on - strand, within Atu4653 at 1807.733 kb on + strand, within Atu4653 at 1807.738 kb on + strand, within Atu4653 at 1807.738 kb on + strand, within Atu4653 at 1807.738 kb on + strand, within Atu4653 at 1807.738 kb on + strand, within Atu4653 at 1807.746 kb on - strand, within Atu4653 at 1807.780 kb on - strand, within Atu4653 at 1808.033 kb on + strand at 1808.084 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrite 9 mM remove 1,805,334 - Atu4651 0.42 -1.9 1,805,345 - Atu4651 0.43 +0.4 1,805,397 - Atu4651 0.47 +0.5 1,805,629 - Atu4651 0.64 -0.2 1,805,647 - Atu4651 0.65 +0.4 1,805,648 + Atu4651 0.65 +0.6 1,805,648 + Atu4651 0.65 +1.0 1,805,765 + Atu4651 0.74 +0.1 1,805,768 - Atu4651 0.74 +0.2 1,805,846 - Atu4651 0.80 +0.1 1,805,877 + Atu4651 0.82 +0.0 1,805,895 + Atu4651 0.83 -0.1 1,805,903 - Atu4651 0.84 +0.5 1,805,937 + Atu4651 0.86 -0.4 1,805,937 + Atu4651 0.86 -0.6 1,805,945 - Atu4651 0.87 +1.2 1,805,999 + -0.4 1,806,028 - -0.4 1,806,042 + +1.2 1,806,125 + -0.2 1,806,130 + -0.0 1,806,133 - +0.5 1,806,155 + -0.3 1,806,265 - +1.0 1,806,293 + +0.1 1,806,341 + +0.2 1,806,349 - +0.1 1,806,411 + Atu4652 0.16 -0.3 1,806,432 + Atu4652 0.19 +0.4 1,806,433 + Atu4652 0.19 -0.8 1,806,433 + Atu4652 0.19 -0.1 1,806,441 - Atu4652 0.20 -0.2 1,806,441 - Atu4652 0.20 +0.6 1,806,471 + Atu4652 0.23 -0.7 1,806,479 - Atu4652 0.24 +1.1 1,806,479 - Atu4652 0.24 -1.0 1,806,479 - Atu4652 0.24 -0.5 1,806,506 - Atu4652 0.28 -0.1 1,806,556 - Atu4652 0.34 +0.4 1,806,592 + Atu4652 0.38 +0.1 1,806,726 - Atu4652 0.54 +0.0 1,806,727 + Atu4652 0.54 +0.3 1,806,815 + Atu4652 0.64 +0.1 1,806,815 + Atu4652 0.64 -0.3 1,806,815 + Atu4652 0.64 +1.2 1,806,823 - Atu4652 0.65 +0.4 1,806,823 - Atu4652 0.65 +1.8 1,806,858 + Atu4652 0.69 +0.3 1,806,875 + Atu4652 0.71 -0.9 1,806,902 - Atu4652 0.75 +0.7 1,806,907 - Atu4652 0.75 -0.8 1,806,958 + Atu4652 0.81 +0.6 1,806,975 - Atu4652 0.83 +0.1 1,807,034 + -0.3 1,807,044 + +0.7 1,807,131 + +0.1 1,807,139 - -2.4 1,807,139 - -0.2 1,807,194 - +0.3 1,807,224 + Atu4653 0.12 +0.4 1,807,293 + Atu4653 0.19 -0.6 1,807,293 + Atu4653 0.19 -0.4 1,807,293 + Atu4653 0.19 -0.2 1,807,293 + Atu4653 0.19 +0.5 1,807,301 - Atu4653 0.20 +0.2 1,807,359 + Atu4653 0.26 +0.2 1,807,398 - Atu4653 0.30 +0.1 1,807,518 + Atu4653 0.43 +0.5 1,807,539 - Atu4653 0.45 +0.4 1,807,552 - Atu4653 0.47 -0.4 1,807,592 - Atu4653 0.51 +1.1 1,807,692 + Atu4653 0.62 -0.3 1,807,706 - Atu4653 0.63 -0.4 1,807,733 + Atu4653 0.66 -0.6 1,807,738 + Atu4653 0.66 -0.8 1,807,738 + Atu4653 0.66 -0.1 1,807,738 + Atu4653 0.66 +0.2 1,807,738 + Atu4653 0.66 -0.9 1,807,746 - Atu4653 0.67 -0.1 1,807,780 - Atu4653 0.71 +0.4 1,808,033 + -0.1 1,808,084 + +1.9
Or see this region's nucleotide sequence