Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_488 and PfGW456L13_489 overlap by 4 nucleotides PfGW456L13_489 and PfGW456L13_490 are separated by 127 nucleotides PfGW456L13_490 and PfGW456L13_491 are separated by 146 nucleotides
PfGW456L13_488 - Mobile element protein, at 558,305 to 558,610
_488
PfGW456L13_489 - Mobile element protein, at 558,607 to 559,443
_489
PfGW456L13_490 - GGDEF domain protein, at 559,571 to 561,490
_490
PfGW456L13_491 - Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1), at 561,637 to 562,821
_491
Position (kb) 559
560
561
562 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 559.467 kb on + strand at 559.467 kb on + strand at 559.529 kb on - strand at 559.572 kb on + strand at 559.573 kb on - strand at 559.573 kb on - strand at 559.573 kb on - strand at 559.573 kb on - strand at 559.573 kb on - strand at 559.855 kb on - strand, within PfGW456L13_490 at 560.023 kb on - strand, within PfGW456L13_490 at 560.280 kb on + strand, within PfGW456L13_490 at 560.281 kb on - strand, within PfGW456L13_490 at 560.281 kb on - strand, within PfGW456L13_490 at 560.283 kb on + strand, within PfGW456L13_490 at 560.284 kb on - strand, within PfGW456L13_490 at 560.284 kb on - strand, within PfGW456L13_490 at 560.373 kb on + strand, within PfGW456L13_490 at 560.373 kb on + strand, within PfGW456L13_490 at 560.382 kb on + strand, within PfGW456L13_490 at 560.495 kb on + strand, within PfGW456L13_490 at 560.518 kb on + strand, within PfGW456L13_490 at 560.518 kb on + strand, within PfGW456L13_490 at 560.600 kb on + strand, within PfGW456L13_490 at 560.600 kb on + strand, within PfGW456L13_490 at 560.601 kb on - strand, within PfGW456L13_490 at 560.601 kb on - strand, within PfGW456L13_490 at 560.601 kb on - strand, within PfGW456L13_490 at 560.601 kb on - strand, within PfGW456L13_490 at 560.702 kb on - strand, within PfGW456L13_490 at 560.702 kb on - strand, within PfGW456L13_490 at 560.702 kb on - strand, within PfGW456L13_490 at 560.919 kb on + strand, within PfGW456L13_490 at 560.919 kb on + strand, within PfGW456L13_490 at 560.919 kb on + strand, within PfGW456L13_490 at 560.919 kb on + strand, within PfGW456L13_490 at 560.920 kb on - strand, within PfGW456L13_490 at 560.976 kb on + strand, within PfGW456L13_490 at 561.057 kb on + strand, within PfGW456L13_490 at 561.057 kb on + strand, within PfGW456L13_490 at 561.058 kb on - strand, within PfGW456L13_490 at 561.345 kb on + strand at 561.345 kb on + strand at 561.345 kb on + strand at 561.401 kb on + strand at 561.408 kb on + strand at 561.447 kb on + strand at 561.447 kb on + strand at 561.447 kb on + strand at 561.447 kb on + strand at 561.447 kb on + strand at 561.447 kb on + strand at 561.448 kb on - strand at 561.477 kb on + strand at 561.478 kb on - strand at 561.478 kb on - strand at 561.526 kb on - strand at 561.526 kb on - strand at 561.528 kb on - strand at 561.532 kb on + strand at 561.532 kb on + strand at 561.533 kb on - strand at 561.554 kb on + strand at 561.554 kb on + strand at 561.555 kb on - strand at 561.555 kb on - strand at 561.555 kb on - strand at 561.555 kb on - strand at 561.555 kb on - strand at 561.588 kb on + strand at 561.589 kb on - strand at 561.589 kb on - strand at 561.589 kb on - strand at 561.589 kb on - strand at 561.595 kb on - strand at 561.630 kb on - strand at 561.636 kb on + strand at 561.636 kb on + strand at 561.636 kb on + strand at 561.637 kb on - strand at 561.637 kb on - strand at 561.637 kb on - strand at 561.637 kb on - strand at 561.637 kb on - strand at 561.637 kb on - strand at 561.644 kb on - strand at 561.644 kb on - strand at 561.730 kb on + strand at 561.731 kb on - strand at 561.731 kb on - strand at 561.731 kb on - strand at 561.731 kb on - strand at 561.731 kb on - strand at 561.731 kb on - strand at 561.731 kb on - strand at 561.853 kb on - strand, within PfGW456L13_491 at 561.853 kb on - strand, within PfGW456L13_491 at 562.025 kb on - strand, within PfGW456L13_491 at 562.254 kb on + strand, within PfGW456L13_491 at 562.254 kb on + strand, within PfGW456L13_491 at 562.255 kb on - strand, within PfGW456L13_491 at 562.255 kb on - strand, within PfGW456L13_491 at 562.255 kb on - strand, within PfGW456L13_491 at 562.255 kb on - strand, within PfGW456L13_491 at 562.270 kb on + strand, within PfGW456L13_491 at 562.270 kb on + strand, within PfGW456L13_491 at 562.270 kb on + strand, within PfGW456L13_491 at 562.270 kb on + strand, within PfGW456L13_491 at 562.270 kb on + strand, within PfGW456L13_491 at 562.271 kb on - strand, within PfGW456L13_491 at 562.271 kb on - strand, within PfGW456L13_491 at 562.275 kb on + strand, within PfGW456L13_491 at 562.275 kb on + strand, within PfGW456L13_491 at 562.276 kb on - strand, within PfGW456L13_491 at 562.276 kb on - strand, within PfGW456L13_491 at 562.469 kb on - strand, within PfGW456L13_491
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 559,467 + -0.4 559,467 + +1.2 559,529 - +0.3 559,572 + +0.9 559,573 - -0.4 559,573 - -0.1 559,573 - +1.1 559,573 - -0.2 559,573 - +0.1 559,855 - PfGW456L13_490 0.15 -0.0 560,023 - PfGW456L13_490 0.24 -0.3 560,280 + PfGW456L13_490 0.37 +0.1 560,281 - PfGW456L13_490 0.37 -0.4 560,281 - PfGW456L13_490 0.37 -0.2 560,283 + PfGW456L13_490 0.37 -1.9 560,284 - PfGW456L13_490 0.37 +1.1 560,284 - PfGW456L13_490 0.37 -1.0 560,373 + PfGW456L13_490 0.42 +1.2 560,373 + PfGW456L13_490 0.42 +0.1 560,382 + PfGW456L13_490 0.42 +1.7 560,495 + PfGW456L13_490 0.48 +0.3 560,518 + PfGW456L13_490 0.49 +0.5 560,518 + PfGW456L13_490 0.49 +0.4 560,600 + PfGW456L13_490 0.54 +0.1 560,600 + PfGW456L13_490 0.54 +0.8 560,601 - PfGW456L13_490 0.54 +1.1 560,601 - PfGW456L13_490 0.54 -0.3 560,601 - PfGW456L13_490 0.54 -0.3 560,601 - PfGW456L13_490 0.54 +0.6 560,702 - PfGW456L13_490 0.59 -0.1 560,702 - PfGW456L13_490 0.59 +0.8 560,702 - PfGW456L13_490 0.59 +1.2 560,919 + PfGW456L13_490 0.70 +0.1 560,919 + PfGW456L13_490 0.70 -2.8 560,919 + PfGW456L13_490 0.70 -0.9 560,919 + PfGW456L13_490 0.70 -2.5 560,920 - PfGW456L13_490 0.70 +0.1 560,976 + PfGW456L13_490 0.73 +0.6 561,057 + PfGW456L13_490 0.77 -0.3 561,057 + PfGW456L13_490 0.77 -1.5 561,058 - PfGW456L13_490 0.77 -0.1 561,345 + -0.6 561,345 + +0.1 561,345 + +0.1 561,401 + +0.1 561,408 + +0.7 561,447 + +0.1 561,447 + -1.5 561,447 + +0.4 561,447 + +0.1 561,447 + +0.5 561,447 + +0.1 561,448 - +0.5 561,477 + +1.1 561,478 - -0.8 561,478 - +0.3 561,526 - -0.2 561,526 - -4.0 561,528 - -0.1 561,532 + -0.1 561,532 + +1.1 561,533 - -1.3 561,554 + +1.3 561,554 + -0.0 561,555 - -0.9 561,555 - -0.4 561,555 - -2.9 561,555 - -1.5 561,555 - -0.9 561,588 + +0.6 561,589 - -0.8 561,589 - -0.7 561,589 - +0.5 561,589 - -0.9 561,595 - +0.6 561,630 - +0.1 561,636 + +0.7 561,636 + -3.1 561,636 + -0.9 561,637 - +0.6 561,637 - +1.1 561,637 - -0.6 561,637 - +0.1 561,637 - -0.3 561,637 - -2.4 561,644 - -2.5 561,644 - +0.9 561,730 + -1.4 561,731 - -0.2 561,731 - -0.6 561,731 - +0.2 561,731 - -0.4 561,731 - -1.0 561,731 - -0.9 561,731 - -0.1 561,853 - PfGW456L13_491 0.18 -0.7 561,853 - PfGW456L13_491 0.18 -1.5 562,025 - PfGW456L13_491 0.33 +0.1 562,254 + PfGW456L13_491 0.52 -0.4 562,254 + PfGW456L13_491 0.52 -2.3 562,255 - PfGW456L13_491 0.52 +0.1 562,255 - PfGW456L13_491 0.52 -0.2 562,255 - PfGW456L13_491 0.52 +0.1 562,255 - PfGW456L13_491 0.52 -0.5 562,270 + PfGW456L13_491 0.53 +1.2 562,270 + PfGW456L13_491 0.53 -2.5 562,270 + PfGW456L13_491 0.53 -3.1 562,270 + PfGW456L13_491 0.53 +0.6 562,270 + PfGW456L13_491 0.53 -0.5 562,271 - PfGW456L13_491 0.54 +1.1 562,271 - PfGW456L13_491 0.54 -0.3 562,275 + PfGW456L13_491 0.54 +0.1 562,275 + PfGW456L13_491 0.54 -1.0 562,276 - PfGW456L13_491 0.54 +0.1 562,276 - PfGW456L13_491 0.54 -0.8 562,469 - PfGW456L13_491 0.70 -2.4
Or see this region's nucleotide sequence