Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_425 and PfGW456L13_426 are separated by 130 nucleotides PfGW456L13_426 and PfGW456L13_427 are separated by 115 nucleotides
PfGW456L13_425 - Ribosomal protein S6 glutaminyl transferase, at 488,655 to 489,560
_425
PfGW456L13_426 - Osmolarity sensory histidine kinase EnvZ, at 489,691 to 491,004
_426
PfGW456L13_427 - Two-component system response regulator OmpR, at 491,120 to 491,860
_427
Position (kb)
489
490
491
492 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 488.691 kb on + strand at 488.691 kb on + strand at 488.691 kb on + strand at 488.691 kb on + strand at 488.692 kb on - strand at 488.766 kb on + strand, within PfGW456L13_425 at 488.766 kb on + strand, within PfGW456L13_425 at 488.766 kb on + strand, within PfGW456L13_425 at 488.767 kb on - strand, within PfGW456L13_425 at 488.767 kb on - strand, within PfGW456L13_425 at 488.767 kb on - strand, within PfGW456L13_425 at 488.767 kb on - strand, within PfGW456L13_425 at 488.767 kb on - strand, within PfGW456L13_425 at 488.767 kb on - strand, within PfGW456L13_425 at 488.868 kb on + strand, within PfGW456L13_425 at 488.868 kb on + strand, within PfGW456L13_425 at 488.868 kb on + strand, within PfGW456L13_425 at 488.868 kb on + strand, within PfGW456L13_425 at 488.868 kb on + strand, within PfGW456L13_425 at 488.869 kb on - strand, within PfGW456L13_425 at 488.869 kb on - strand, within PfGW456L13_425 at 488.869 kb on - strand, within PfGW456L13_425 at 488.869 kb on - strand, within PfGW456L13_425 at 488.869 kb on - strand, within PfGW456L13_425 at 488.869 kb on - strand, within PfGW456L13_425 at 488.908 kb on + strand, within PfGW456L13_425 at 488.908 kb on + strand, within PfGW456L13_425 at 488.908 kb on + strand, within PfGW456L13_425 at 488.908 kb on + strand, within PfGW456L13_425 at 488.908 kb on + strand, within PfGW456L13_425 at 489.106 kb on + strand, within PfGW456L13_425 at 489.107 kb on - strand, within PfGW456L13_425 at 489.107 kb on - strand, within PfGW456L13_425 at 489.107 kb on - strand, within PfGW456L13_425 at 489.189 kb on + strand, within PfGW456L13_425 at 489.189 kb on + strand, within PfGW456L13_425 at 489.189 kb on + strand, within PfGW456L13_425 at 489.189 kb on + strand, within PfGW456L13_425 at 489.189 kb on + strand, within PfGW456L13_425 at 489.189 kb on + strand, within PfGW456L13_425 at 489.189 kb on + strand, within PfGW456L13_425 at 489.190 kb on - strand, within PfGW456L13_425 at 489.190 kb on - strand, within PfGW456L13_425 at 489.190 kb on - strand, within PfGW456L13_425 at 489.355 kb on + strand, within PfGW456L13_425 at 489.355 kb on + strand, within PfGW456L13_425 at 489.437 kb on + strand, within PfGW456L13_425 at 489.437 kb on + strand, within PfGW456L13_425 at 489.437 kb on + strand, within PfGW456L13_425 at 489.438 kb on - strand, within PfGW456L13_425 at 489.438 kb on - strand, within PfGW456L13_425 at 489.533 kb on + strand at 489.558 kb on + strand at 489.624 kb on - strand at 489.624 kb on - strand at 489.934 kb on + strand, within PfGW456L13_426 at 489.934 kb on + strand, within PfGW456L13_426 at 489.934 kb on + strand, within PfGW456L13_426 at 489.934 kb on + strand, within PfGW456L13_426 at 489.935 kb on - strand, within PfGW456L13_426 at 489.935 kb on - strand, within PfGW456L13_426 at 489.935 kb on - strand, within PfGW456L13_426 at 489.935 kb on - strand, within PfGW456L13_426 at 489.938 kb on + strand, within PfGW456L13_426 at 489.938 kb on + strand, within PfGW456L13_426 at 489.938 kb on + strand, within PfGW456L13_426 at 489.938 kb on + strand, within PfGW456L13_426 at 489.938 kb on + strand, within PfGW456L13_426 at 489.939 kb on - strand, within PfGW456L13_426 at 489.939 kb on - strand, within PfGW456L13_426 at 489.952 kb on - strand, within PfGW456L13_426 at 489.962 kb on - strand, within PfGW456L13_426 at 489.966 kb on - strand, within PfGW456L13_426 at 489.966 kb on - strand, within PfGW456L13_426 at 489.966 kb on - strand, within PfGW456L13_426 at 489.966 kb on - strand, within PfGW456L13_426 at 489.966 kb on - strand, within PfGW456L13_426 at 490.417 kb on - strand, within PfGW456L13_426 at 490.470 kb on - strand, within PfGW456L13_426 at 490.470 kb on - strand, within PfGW456L13_426 at 490.542 kb on + strand, within PfGW456L13_426 at 490.722 kb on - strand, within PfGW456L13_426 at 490.722 kb on - strand, within PfGW456L13_426 at 490.722 kb on - strand, within PfGW456L13_426 at 490.734 kb on - strand, within PfGW456L13_426 at 490.836 kb on - strand, within PfGW456L13_426 at 490.937 kb on - strand at 490.937 kb on - strand at 491.107 kb on + strand at 491.107 kb on + strand at 491.108 kb on - strand at 491.108 kb on - strand at 491.108 kb on - strand at 491.154 kb on + strand at 491.182 kb on - strand at 491.340 kb on - strand, within PfGW456L13_427 at 491.340 kb on - strand, within PfGW456L13_427 at 491.416 kb on - strand, within PfGW456L13_427 at 491.889 kb on + strand at 491.897 kb on - strand at 491.897 kb on - strand at 491.898 kb on - strand at 491.906 kb on + strand at 491.908 kb on + strand at 491.908 kb on + strand at 491.908 kb on + strand at 491.908 kb on + strand at 491.908 kb on + strand at 491.909 kb on - strand at 491.909 kb on - strand at 491.909 kb on - strand at 491.909 kb on - strand at 491.909 kb on - strand at 491.909 kb on - strand at 491.909 kb on - strand at 491.909 kb on - strand at 491.928 kb on - strand at 491.943 kb on + strand at 491.943 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 488,691 + -1.5 488,691 + +1.8 488,691 + -1.9 488,691 + -1.3 488,692 - +0.4 488,766 + PfGW456L13_425 0.12 +0.3 488,766 + PfGW456L13_425 0.12 -1.4 488,766 + PfGW456L13_425 0.12 +2.1 488,767 - PfGW456L13_425 0.12 +0.2 488,767 - PfGW456L13_425 0.12 -0.8 488,767 - PfGW456L13_425 0.12 -0.9 488,767 - PfGW456L13_425 0.12 +0.1 488,767 - PfGW456L13_425 0.12 +1.0 488,767 - PfGW456L13_425 0.12 -1.1 488,868 + PfGW456L13_425 0.24 -0.8 488,868 + PfGW456L13_425 0.24 -0.2 488,868 + PfGW456L13_425 0.24 -0.4 488,868 + PfGW456L13_425 0.24 -0.1 488,868 + PfGW456L13_425 0.24 +0.3 488,869 - PfGW456L13_425 0.24 -0.1 488,869 - PfGW456L13_425 0.24 +0.4 488,869 - PfGW456L13_425 0.24 +0.1 488,869 - PfGW456L13_425 0.24 -0.9 488,869 - PfGW456L13_425 0.24 -1.4 488,869 - PfGW456L13_425 0.24 +1.1 488,908 + PfGW456L13_425 0.28 +1.6 488,908 + PfGW456L13_425 0.28 +1.6 488,908 + PfGW456L13_425 0.28 +0.6 488,908 + PfGW456L13_425 0.28 +0.8 488,908 + PfGW456L13_425 0.28 +0.4 489,106 + PfGW456L13_425 0.50 -0.3 489,107 - PfGW456L13_425 0.50 +0.6 489,107 - PfGW456L13_425 0.50 +0.1 489,107 - PfGW456L13_425 0.50 -0.3 489,189 + PfGW456L13_425 0.59 +0.2 489,189 + PfGW456L13_425 0.59 -1.9 489,189 + PfGW456L13_425 0.59 +1.2 489,189 + PfGW456L13_425 0.59 +0.9 489,189 + PfGW456L13_425 0.59 +1.1 489,189 + PfGW456L13_425 0.59 -1.4 489,189 + PfGW456L13_425 0.59 +0.7 489,190 - PfGW456L13_425 0.59 -0.9 489,190 - PfGW456L13_425 0.59 -0.2 489,190 - PfGW456L13_425 0.59 -2.5 489,355 + PfGW456L13_425 0.77 +1.0 489,355 + PfGW456L13_425 0.77 +0.3 489,437 + PfGW456L13_425 0.86 -1.5 489,437 + PfGW456L13_425 0.86 +1.1 489,437 + PfGW456L13_425 0.86 +0.8 489,438 - PfGW456L13_425 0.86 -0.4 489,438 - PfGW456L13_425 0.86 +0.1 489,533 + -1.3 489,558 + +0.3 489,624 - +0.2 489,624 - -0.7 489,934 + PfGW456L13_426 0.18 -0.8 489,934 + PfGW456L13_426 0.18 +0.1 489,934 + PfGW456L13_426 0.18 -1.9 489,934 + PfGW456L13_426 0.18 -0.9 489,935 - PfGW456L13_426 0.19 +2.1 489,935 - PfGW456L13_426 0.19 -0.5 489,935 - PfGW456L13_426 0.19 +0.3 489,935 - PfGW456L13_426 0.19 +1.1 489,938 + PfGW456L13_426 0.19 -3.4 489,938 + PfGW456L13_426 0.19 -1.9 489,938 + PfGW456L13_426 0.19 +2.1 489,938 + PfGW456L13_426 0.19 -0.9 489,938 + PfGW456L13_426 0.19 -0.9 489,939 - PfGW456L13_426 0.19 +0.1 489,939 - PfGW456L13_426 0.19 -0.4 489,952 - PfGW456L13_426 0.20 -2.5 489,962 - PfGW456L13_426 0.21 -2.3 489,966 - PfGW456L13_426 0.21 -1.3 489,966 - PfGW456L13_426 0.21 +0.1 489,966 - PfGW456L13_426 0.21 +2.6 489,966 - PfGW456L13_426 0.21 +1.1 489,966 - PfGW456L13_426 0.21 -1.9 490,417 - PfGW456L13_426 0.55 +0.1 490,470 - PfGW456L13_426 0.59 +0.1 490,470 - PfGW456L13_426 0.59 +0.1 490,542 + PfGW456L13_426 0.65 -0.9 490,722 - PfGW456L13_426 0.78 +1.3 490,722 - PfGW456L13_426 0.78 -1.7 490,722 - PfGW456L13_426 0.78 -1.5 490,734 - PfGW456L13_426 0.79 -0.9 490,836 - PfGW456L13_426 0.87 -1.9 490,937 - -0.5 490,937 - +2.4 491,107 + -0.0 491,107 + +2.8 491,108 - -0.9 491,108 - -0.3 491,108 - -0.8 491,154 + +0.1 491,182 - +0.1 491,340 - PfGW456L13_427 0.30 -0.9 491,340 - PfGW456L13_427 0.30 +0.1 491,416 - PfGW456L13_427 0.40 +1.3 491,889 + +2.6 491,897 - +0.6 491,897 - +3.2 491,898 - -1.5 491,906 + -0.2 491,908 + +1.9 491,908 + +0.7 491,908 + -0.8 491,908 + -1.2 491,908 + +0.6 491,909 - +0.7 491,909 - -1.6 491,909 - +1.1 491,909 - -0.9 491,909 - -0.4 491,909 - -2.6 491,909 - +0.4 491,909 - +0.6 491,928 - +0.6 491,943 + -0.3 491,943 + -1.3
Or see this region's nucleotide sequence