Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_3891 and PfGW456L13_3892 are separated by 750 nucleotides PfGW456L13_3892 and PfGW456L13_5218 are separated by 92 nucleotides PfGW456L13_5218 and PfGW456L13_3893 are separated by 89 nucleotides PfGW456L13_3893 and PfGW456L13_3894 overlap by 20 nucleotides PfGW456L13_3894 and PfGW456L13_3895 are separated by 3 nucleotides
PfGW456L13_3891 - hypothetical protein, at 4,368,460 to 4,368,588
_3891
PfGW456L13_3892 - Integrase, at 4,369,339 to 4,369,833
_3892
PfGW456L13_5218 - tRNA-Pro-GGG, at 4,369,926 to 4,369,999
_5218
PfGW456L13_3893 - Transcriptional regulator, MerR family, at 4,370,089 to 4,370,445
_3893
PfGW456L13_3894 - Integration host factor alpha subunit, at 4,370,426 to 4,370,728
_3894
PfGW456L13_3895 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 4,370,732 to 4,373,110
_3895
Position (kb) 4369
4370 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4368.381 kb on + strand at 4368.382 kb on - strand at 4368.382 kb on - strand at 4368.382 kb on - strand at 4368.382 kb on - strand at 4368.382 kb on - strand at 4368.388 kb on + strand at 4368.389 kb on - strand at 4368.389 kb on - strand at 4368.405 kb on + strand at 4368.405 kb on + strand at 4368.405 kb on + strand at 4368.405 kb on + strand at 4368.406 kb on - strand at 4368.406 kb on - strand at 4368.406 kb on - strand at 4368.406 kb on - strand at 4368.406 kb on - strand at 4368.406 kb on - strand at 4368.468 kb on + strand at 4368.468 kb on + strand at 4368.534 kb on + strand, within PfGW456L13_3891 at 4368.535 kb on - strand, within PfGW456L13_3891 at 4368.631 kb on + strand at 4368.631 kb on + strand at 4368.631 kb on + strand at 4368.631 kb on + strand at 4368.631 kb on + strand at 4368.631 kb on + strand at 4368.631 kb on + strand at 4368.632 kb on - strand at 4368.689 kb on - strand at 4368.696 kb on + strand at 4368.697 kb on - strand at 4368.697 kb on - strand at 4368.697 kb on - strand at 4368.781 kb on + strand at 4368.843 kb on + strand at 4368.844 kb on - strand at 4368.980 kb on + strand at 4368.980 kb on + strand at 4368.981 kb on - strand at 4368.981 kb on - strand at 4368.981 kb on - strand at 4368.986 kb on + strand at 4368.987 kb on - strand at 4368.987 kb on - strand at 4368.987 kb on - strand at 4369.000 kb on + strand at 4369.000 kb on + strand at 4369.001 kb on - strand at 4369.001 kb on - strand at 4369.001 kb on - strand at 4369.018 kb on + strand at 4369.018 kb on + strand at 4369.018 kb on + strand at 4369.018 kb on + strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.019 kb on - strand at 4369.052 kb on + strand at 4369.053 kb on - strand at 4369.053 kb on - strand at 4369.053 kb on - strand at 4369.053 kb on - strand at 4369.053 kb on - strand at 4369.122 kb on - strand at 4369.122 kb on - strand at 4369.152 kb on + strand at 4369.153 kb on - strand at 4369.153 kb on - strand at 4369.184 kb on + strand at 4369.199 kb on + strand at 4369.200 kb on - strand at 4369.200 kb on - strand at 4369.207 kb on + strand at 4369.208 kb on - strand at 4369.208 kb on - strand at 4369.257 kb on + strand at 4369.257 kb on + strand at 4369.257 kb on + strand at 4369.258 kb on - strand at 4369.258 kb on - strand at 4369.305 kb on + strand at 4369.330 kb on + strand at 4369.331 kb on - strand at 4369.331 kb on - strand at 4369.352 kb on + strand at 4369.352 kb on + strand at 4369.352 kb on + strand at 4369.352 kb on + strand at 4369.353 kb on - strand at 4369.353 kb on - strand at 4369.353 kb on - strand at 4369.450 kb on + strand, within PfGW456L13_3892 at 4369.450 kb on + strand, within PfGW456L13_3892 at 4369.450 kb on + strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.451 kb on - strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.582 kb on + strand, within PfGW456L13_3892 at 4369.583 kb on - strand, within PfGW456L13_3892 at 4369.583 kb on - strand at 4369.627 kb on + strand, within PfGW456L13_3892 at 4369.627 kb on + strand, within PfGW456L13_3892 at 4369.759 kb on + strand, within PfGW456L13_3892 at 4369.812 kb on + strand at 4369.886 kb on - strand at 4369.917 kb on + strand at 4369.917 kb on + strand at 4369.958 kb on - strand, within PfGW456L13_5218 at 4369.970 kb on + strand, within PfGW456L13_5218 at 4369.971 kb on - strand, within PfGW456L13_5218 at 4370.002 kb on + strand at 4370.047 kb on - strand at 4370.153 kb on + strand, within PfGW456L13_3893 at 4370.153 kb on + strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.154 kb on - strand, within PfGW456L13_3893 at 4370.229 kb on - strand, within PfGW456L13_3893 at 4370.277 kb on - strand, within PfGW456L13_3893 at 4370.356 kb on + strand, within PfGW456L13_3893 at 4370.393 kb on + strand, within PfGW456L13_3893 at 4370.394 kb on - strand, within PfGW456L13_3893 at 4370.445 kb on + strand at 4370.445 kb on + strand at 4370.446 kb on - strand at 4370.446 kb on - strand at 4370.446 kb on - strand at 4370.688 kb on + strand, within PfGW456L13_3894 at 4370.689 kb on - strand, within PfGW456L13_3894 at 4370.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 4,368,381 + -0.9 4,368,382 - +0.8 4,368,382 - +0.3 4,368,382 - -0.3 4,368,382 - +0.7 4,368,382 - -1.0 4,368,388 + -0.3 4,368,389 - +0.2 4,368,389 - +0.3 4,368,405 + +0.2 4,368,405 + +0.6 4,368,405 + +0.7 4,368,405 + -0.8 4,368,406 - +0.1 4,368,406 - +4.3 4,368,406 - -1.4 4,368,406 - -0.2 4,368,406 - +0.5 4,368,406 - -0.4 4,368,468 + -0.8 4,368,468 + -0.6 4,368,534 + PfGW456L13_3891 0.57 +0.7 4,368,535 - PfGW456L13_3891 0.58 -0.3 4,368,631 + -0.8 4,368,631 + -0.2 4,368,631 + -0.7 4,368,631 + -0.6 4,368,631 + -0.1 4,368,631 + +0.3 4,368,631 + -0.5 4,368,632 - -1.1 4,368,689 - +0.4 4,368,696 + +0.3 4,368,697 - -0.4 4,368,697 - -1.2 4,368,697 - +0.4 4,368,781 + +0.7 4,368,843 + +0.8 4,368,844 - -0.1 4,368,980 + -0.0 4,368,980 + +2.0 4,368,981 - -0.8 4,368,981 - -0.0 4,368,981 - +0.7 4,368,986 + -0.0 4,368,987 - +1.6 4,368,987 - -0.0 4,368,987 - +1.8 4,369,000 + -0.6 4,369,000 + -0.8 4,369,001 - -0.1 4,369,001 - -0.2 4,369,001 - +0.1 4,369,018 + -1.6 4,369,018 + -1.0 4,369,018 + +0.3 4,369,018 + -0.0 4,369,019 - +0.2 4,369,019 - -1.8 4,369,019 - +0.5 4,369,019 - +0.2 4,369,019 - +0.1 4,369,019 - -0.3 4,369,019 - +0.8 4,369,019 - -0.7 4,369,019 - +1.1 4,369,052 + -0.0 4,369,053 - -0.8 4,369,053 - +0.5 4,369,053 - -0.5 4,369,053 - -1.8 4,369,053 - +0.4 4,369,122 - -1.0 4,369,122 - -2.0 4,369,152 + -0.3 4,369,153 - +1.8 4,369,153 - +0.5 4,369,184 + -0.0 4,369,199 + -1.2 4,369,200 - +0.1 4,369,200 - +0.2 4,369,207 + -0.6 4,369,208 - -0.9 4,369,208 - +1.1 4,369,257 + -0.6 4,369,257 + +0.5 4,369,257 + +1.8 4,369,258 - +1.0 4,369,258 - +0.2 4,369,305 + +0.5 4,369,330 + +1.1 4,369,331 - -0.1 4,369,331 - -1.4 4,369,352 + +2.0 4,369,352 + -0.6 4,369,352 + +0.7 4,369,352 + -1.0 4,369,353 - -0.5 4,369,353 - +0.9 4,369,353 - -1.7 4,369,450 + PfGW456L13_3892 0.22 +0.2 4,369,450 + PfGW456L13_3892 0.22 +0.3 4,369,450 + PfGW456L13_3892 0.22 -0.4 4,369,451 - PfGW456L13_3892 0.23 +0.4 4,369,451 - PfGW456L13_3892 0.23 +1.4 4,369,451 - PfGW456L13_3892 0.23 -0.0 4,369,451 - PfGW456L13_3892 0.23 +0.1 4,369,451 - PfGW456L13_3892 0.23 +0.1 4,369,451 - PfGW456L13_3892 0.23 +0.2 4,369,451 - PfGW456L13_3892 0.23 +0.7 4,369,582 + PfGW456L13_3892 0.49 -0.4 4,369,582 + PfGW456L13_3892 0.49 +0.4 4,369,582 + PfGW456L13_3892 0.49 +1.1 4,369,582 + PfGW456L13_3892 0.49 +2.6 4,369,582 + PfGW456L13_3892 0.49 -0.0 4,369,583 - PfGW456L13_3892 0.49 -1.0 4,369,583 - +1.0 4,369,627 + PfGW456L13_3892 0.58 -0.5 4,369,627 + PfGW456L13_3892 0.58 +1.2 4,369,759 + PfGW456L13_3892 0.85 +1.4 4,369,812 + +1.7 4,369,886 - -2.0 4,369,917 + -0.0 4,369,917 + -1.6 4,369,958 - PfGW456L13_5218 0.43 -0.0 4,369,970 + PfGW456L13_5218 0.59 -0.0 4,369,971 - PfGW456L13_5218 0.61 -1.1 4,370,002 + +2.3 4,370,047 - -0.3 4,370,153 + PfGW456L13_3893 0.18 -1.6 4,370,153 + PfGW456L13_3893 0.18 -0.5 4,370,154 - PfGW456L13_3893 0.18 -0.0 4,370,154 - PfGW456L13_3893 0.18 -1.0 4,370,154 - PfGW456L13_3893 0.18 -0.9 4,370,154 - PfGW456L13_3893 0.18 +0.8 4,370,229 - PfGW456L13_3893 0.39 -1.0 4,370,277 - PfGW456L13_3893 0.53 -0.3 4,370,356 + PfGW456L13_3893 0.75 +1.0 4,370,393 + PfGW456L13_3893 0.85 +1.0 4,370,394 - PfGW456L13_3893 0.85 +0.3 4,370,445 + -0.0 4,370,445 + -1.6 4,370,446 - -1.0 4,370,446 - -2.0 4,370,446 - -0.0 4,370,688 + PfGW456L13_3894 0.86 -0.0 4,370,689 - PfGW456L13_3894 0.87 -2.0 4,370,729 - -0.0
Or see this region's nucleotide sequence