Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt PfGW456L13_3387 and PfGW456L13_3388 are separated by 42 nucleotides PfGW456L13_3388 and PfGW456L13_3389 overlap by 4 nucleotides PfGW456L13_3389 and PfGW456L13_3390 overlap by 1 nucleotides  
        
        PfGW456L13_3387 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2), at 3,803,999 to 3,805,123 
         
        _3387 
         
        
        PfGW456L13_3388 - Enoyl-CoA hydratase (EC 4.2.1.17), at 3,805,166 to 3,805,966 
         
        _3388 
         
        
        PfGW456L13_3389 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2), at 3,805,963 to 3,807,036 
         
        _3389 
         
        
        PfGW456L13_3390 - Acyl-CoA dehydrogenase, long-chain specific, mitochondrial precursor (EC 1.3.99.13), at 3,807,036 to 3,808,184 
         
        _3390 
         Position (kb)  
3805 
 
3806 
 
3807 
 
3808 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 3805.044 kb on + strand at 3805.045 kb on - strand at 3805.112 kb on + strand at 3805.113 kb on - strand at 3805.164 kb on - strand at 3805.164 kb on - strand at 3805.291 kb on + strand, within PfGW456L13_3388 at 3805.291 kb on + strand, within PfGW456L13_3388 at 3805.291 kb on + strand, within PfGW456L13_3388 at 3805.292 kb on - strand, within PfGW456L13_3388 at 3805.292 kb on - strand, within PfGW456L13_3388 at 3805.292 kb on - strand, within PfGW456L13_3388 at 3805.292 kb on - strand, within PfGW456L13_3388 at 3805.292 kb on - strand, within PfGW456L13_3388 at 3805.292 kb on - strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.445 kb on + strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.446 kb on - strand, within PfGW456L13_3388 at 3805.668 kb on + strand, within PfGW456L13_3388 at 3805.669 kb on - strand, within PfGW456L13_3388 at 3805.669 kb on - strand, within PfGW456L13_3388 at 3805.684 kb on + strand, within PfGW456L13_3388 at 3805.685 kb on - strand, within PfGW456L13_3388 at 3805.704 kb on + strand, within PfGW456L13_3388 at 3805.705 kb on - strand, within PfGW456L13_3388 at 3805.705 kb on - strand, within PfGW456L13_3388 at 3805.705 kb on - strand, within PfGW456L13_3388 at 3805.708 kb on + strand, within PfGW456L13_3388 at 3805.709 kb on - strand, within PfGW456L13_3388 at 3805.723 kb on - strand, within PfGW456L13_3388 at 3805.838 kb on + strand, within PfGW456L13_3388 at 3805.838 kb on + strand, within PfGW456L13_3388 at 3805.838 kb on + strand, within PfGW456L13_3388 at 3806.027 kb on + strand at 3806.388 kb on - strand at 3806.388 kb on - strand, within PfGW456L13_3389 at 3806.407 kb on + strand, within PfGW456L13_3389 at 3806.510 kb on - strand, within PfGW456L13_3389 at 3806.589 kb on + strand, within PfGW456L13_3389 at 3806.679 kb on - strand, within PfGW456L13_3389 at 3806.849 kb on + strand, within PfGW456L13_3389 at 3806.849 kb on + strand, within PfGW456L13_3389 at 3806.849 kb on + strand, within PfGW456L13_3389 at 3806.849 kb on + strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.850 kb on - strand, within PfGW456L13_3389 at 3806.853 kb on + strand, within PfGW456L13_3389 at 3806.853 kb on + strand, within PfGW456L13_3389 at 3806.853 kb on + strand, within PfGW456L13_3389 at 3806.853 kb on + strand, within PfGW456L13_3389 at 3806.853 kb on + strand, within PfGW456L13_3389 at 3806.854 kb on - strand, within PfGW456L13_3389 at 3806.854 kb on - strand, within PfGW456L13_3389 at 3806.854 kb on - strand, within PfGW456L13_3389 at 3806.854 kb on - strand, within PfGW456L13_3389 at 3806.854 kb on - strand, within PfGW456L13_3389 at 3806.854 kb on - strand, within PfGW456L13_3389 at 3806.936 kb on - strand at 3806.972 kb on - strand at 3806.972 kb on - strand at 3806.972 kb on - strand at 3806.972 kb on - strand at 3806.972 kb on - strand at 3806.972 kb on - strand at 3806.975 kb on + strand at 3807.026 kb on - strand at 3807.127 kb on + strand at 3807.302 kb on + strand, within PfGW456L13_3390 at 3807.303 kb on - strand, within PfGW456L13_3390 at 3807.303 kb on - strand, within PfGW456L13_3390 at 3807.354 kb on + strand, within PfGW456L13_3390 at 3807.670 kb on + strand, within PfGW456L13_3390 at 3807.671 kb on - strand, within PfGW456L13_3390 at 3807.671 kb on - strand, within PfGW456L13_3390 at 3807.725 kb on + strand, within PfGW456L13_3390 at 3807.805 kb on + strand, within PfGW456L13_3390 at 3807.806 kb on - strand, within PfGW456L13_3390 at 3807.806 kb on - strand, within PfGW456L13_3390 at 3807.835 kb on + strand, within PfGW456L13_3390 at 3807.953 kb on - strand, within PfGW456L13_3390 at 3807.953 kb on - strand, within PfGW456L13_3390 at 3807.986 kb on - strand, within PfGW456L13_3390  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x             remove   3,805,044   +        +0.7   3,805,045   -        -0.1   3,805,112   +        -1.3   3,805,113   -        +0.6   3,805,164   -        -0.2   3,805,164   -        -0.5   3,805,291   +    PfGW456L13_3388   0.16  -0.2   3,805,291   +    PfGW456L13_3388   0.16  +0.6   3,805,291   +    PfGW456L13_3388   0.16  -0.4   3,805,292   -    PfGW456L13_3388   0.16  +0.9   3,805,292   -    PfGW456L13_3388   0.16  -0.0   3,805,292   -    PfGW456L13_3388   0.16  -0.2   3,805,292   -    PfGW456L13_3388   0.16  +0.4   3,805,292   -    PfGW456L13_3388   0.16  -1.6   3,805,292   -    PfGW456L13_3388   0.16  +0.1   3,805,445   +    PfGW456L13_3388   0.35  -0.8   3,805,445   +    PfGW456L13_3388   0.35  -0.3   3,805,445   +    PfGW456L13_3388   0.35  +0.4   3,805,445   +        -0.0   3,805,445   +    PfGW456L13_3388   0.35  -1.2   3,805,445   +    PfGW456L13_3388   0.35  -1.2   3,805,445   +    PfGW456L13_3388   0.35  -0.9   3,805,445   +    PfGW456L13_3388   0.35  +2.6   3,805,445   +    PfGW456L13_3388   0.35  -0.6   3,805,445   +    PfGW456L13_3388   0.35  -1.6   3,805,446   -    PfGW456L13_3388   0.35  -1.5   3,805,446   -    PfGW456L13_3388   0.35  -0.6   3,805,446   -    PfGW456L13_3388   0.35  -0.0   3,805,446   -    PfGW456L13_3388   0.35  -0.4   3,805,446   -    PfGW456L13_3388   0.35  +1.4   3,805,446   -    PfGW456L13_3388   0.35  -0.1   3,805,446   -    PfGW456L13_3388   0.35  -0.7   3,805,446   -    PfGW456L13_3388   0.35  +0.2   3,805,446   -    PfGW456L13_3388   0.35  -0.4   3,805,446   -    PfGW456L13_3388   0.35  -0.8   3,805,668   +    PfGW456L13_3388   0.63  -1.7   3,805,669   -    PfGW456L13_3388   0.63  -2.0   3,805,669   -    PfGW456L13_3388   0.63  -0.2   3,805,684   +    PfGW456L13_3388   0.65  -0.3   3,805,685   -    PfGW456L13_3388   0.65  -0.0   3,805,704   +    PfGW456L13_3388   0.67  +1.0   3,805,705   -    PfGW456L13_3388   0.67  -1.4   3,805,705   -    PfGW456L13_3388   0.67  +0.5   3,805,705   -    PfGW456L13_3388   0.67  +1.2   3,805,708   +    PfGW456L13_3388   0.68  +0.5   3,805,709   -    PfGW456L13_3388   0.68  -0.6   3,805,723   -    PfGW456L13_3388   0.70  -1.4   3,805,838   +    PfGW456L13_3388   0.84  -0.6   3,805,838   +    PfGW456L13_3388   0.84  +1.3   3,805,838   +    PfGW456L13_3388   0.84  -0.1   3,806,027   +        -0.0   3,806,388   -        -0.6   3,806,388   -    PfGW456L13_3389   0.40  -0.1   3,806,407   +    PfGW456L13_3389   0.41  -1.0   3,806,510   -    PfGW456L13_3389   0.51  -0.4   3,806,589   +    PfGW456L13_3389   0.58  -0.0   3,806,679   -    PfGW456L13_3389   0.67  -0.2   3,806,849   +    PfGW456L13_3389   0.82  +0.4   3,806,849   +    PfGW456L13_3389   0.82  +1.3   3,806,849   +    PfGW456L13_3389   0.82  -0.3   3,806,849   +    PfGW456L13_3389   0.82  +0.6   3,806,850   -    PfGW456L13_3389   0.83  +1.3   3,806,850   -    PfGW456L13_3389   0.83  -0.0   3,806,850   -    PfGW456L13_3389   0.83  -0.6   3,806,850   -    PfGW456L13_3389   0.83  -0.9   3,806,850   -    PfGW456L13_3389   0.83  -1.0   3,806,850   -    PfGW456L13_3389   0.83  -2.4   3,806,850   -        +0.6   3,806,850   -    PfGW456L13_3389   0.83  +0.3   3,806,850   -    PfGW456L13_3389   0.83  -0.6   3,806,850   -    PfGW456L13_3389   0.83  -0.3   3,806,850   -    PfGW456L13_3389   0.83  -0.4   3,806,850   -        +0.6   3,806,850   -    PfGW456L13_3389   0.83  -2.5   3,806,850   -    PfGW456L13_3389   0.83  +1.2   3,806,850   -        -2.3   3,806,850   -    PfGW456L13_3389   0.83  +0.7   3,806,850   -    PfGW456L13_3389   0.83  -0.7   3,806,850   -    PfGW456L13_3389   0.83  -0.7   3,806,850   -    PfGW456L13_3389   0.83  +0.3   3,806,850   -    PfGW456L13_3389   0.83  +0.2   3,806,850   -    PfGW456L13_3389   0.83  -0.5   3,806,850   -    PfGW456L13_3389   0.83  -0.1   3,806,850   -    PfGW456L13_3389   0.83  +0.7   3,806,850   -    PfGW456L13_3389   0.83  -1.0   3,806,850   -    PfGW456L13_3389   0.83  -0.5   3,806,853   +    PfGW456L13_3389   0.83  +0.7   3,806,853   +    PfGW456L13_3389   0.83  +1.0   3,806,853   +    PfGW456L13_3389   0.83  -1.9   3,806,853   +    PfGW456L13_3389   0.83  -1.0   3,806,853   +    PfGW456L13_3389   0.83  +0.3   3,806,854   -    PfGW456L13_3389   0.83  +0.2   3,806,854   -    PfGW456L13_3389   0.83  -0.7   3,806,854   -    PfGW456L13_3389   0.83  -0.0   3,806,854   -    PfGW456L13_3389   0.83  -1.9   3,806,854   -    PfGW456L13_3389   0.83  -0.1   3,806,854   -    PfGW456L13_3389   0.83  +0.7   3,806,936   -        -0.6   3,806,972   -        -0.3   3,806,972   -        -0.7   3,806,972   -        -0.6   3,806,972   -        -0.1   3,806,972   -        -0.6   3,806,972   -        -0.0   3,806,975   +        +0.4   3,807,026   -        +0.3   3,807,127   +        -1.4   3,807,302   +    PfGW456L13_3390   0.23  -0.0   3,807,303   -    PfGW456L13_3390   0.23  +0.4   3,807,303   -    PfGW456L13_3390   0.23  -1.0   3,807,354   +    PfGW456L13_3390   0.28  -0.3   3,807,670   +    PfGW456L13_3390   0.55  +0.1   3,807,671   -    PfGW456L13_3390   0.55  +0.3   3,807,671   -    PfGW456L13_3390   0.55  +0.2   3,807,725   +    PfGW456L13_3390   0.60  +0.4   3,807,805   +    PfGW456L13_3390   0.67  -0.9   3,807,806   -    PfGW456L13_3390   0.67  +0.8   3,807,806   -    PfGW456L13_3390   0.67  -0.2   3,807,835   +    PfGW456L13_3390   0.70  +0.3   3,807,953   -    PfGW456L13_3390   0.80  +1.4   3,807,953   -    PfGW456L13_3390   0.80  -2.0   3,807,986   -    PfGW456L13_3390   0.83  -0.6 
 
Or see this region's nucleotide sequence