Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_3089 and PfGW456L13_3090 are separated by 198 nucleotides PfGW456L13_3090 and PfGW456L13_3091 are separated by 289 nucleotides
PfGW456L13_3089 - sodium/hydrogen exchanger family protein, at 3,443,763 to 3,445,124
_3089
PfGW456L13_3090 - putative aminotransferase, at 3,445,323 to 3,446,477
_3090
PfGW456L13_3091 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 3,446,767 to 3,449,832
_3091
Position (kb)
3445
3446
3447 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3444.395 kb on - strand at 3444.395 kb on - strand, within PfGW456L13_3089 at 3444.424 kb on + strand, within PfGW456L13_3089 at 3444.424 kb on + strand, within PfGW456L13_3089 at 3444.424 kb on + strand, within PfGW456L13_3089 at 3444.425 kb on - strand, within PfGW456L13_3089 at 3444.425 kb on - strand, within PfGW456L13_3089 at 3444.425 kb on - strand, within PfGW456L13_3089 at 3444.641 kb on + strand, within PfGW456L13_3089 at 3444.641 kb on + strand, within PfGW456L13_3089 at 3444.641 kb on + strand, within PfGW456L13_3089 at 3445.057 kb on + strand at 3445.058 kb on - strand at 3445.159 kb on + strand at 3445.159 kb on + strand at 3445.161 kb on + strand at 3445.161 kb on + strand at 3445.161 kb on + strand at 3445.161 kb on + strand at 3445.161 kb on + strand at 3445.162 kb on - strand at 3445.162 kb on - strand at 3445.162 kb on - strand at 3445.162 kb on - strand at 3445.198 kb on + strand at 3445.306 kb on - strand at 3445.311 kb on + strand at 3445.528 kb on + strand, within PfGW456L13_3090 at 3445.528 kb on + strand, within PfGW456L13_3090 at 3445.528 kb on + strand, within PfGW456L13_3090 at 3445.529 kb on - strand, within PfGW456L13_3090 at 3445.575 kb on + strand, within PfGW456L13_3090 at 3445.575 kb on + strand, within PfGW456L13_3090 at 3445.576 kb on - strand, within PfGW456L13_3090 at 3445.591 kb on + strand, within PfGW456L13_3090 at 3445.591 kb on + strand, within PfGW456L13_3090 at 3445.592 kb on - strand, within PfGW456L13_3090 at 3445.612 kb on + strand, within PfGW456L13_3090 at 3445.612 kb on + strand, within PfGW456L13_3090 at 3445.652 kb on - strand, within PfGW456L13_3090 at 3445.652 kb on - strand, within PfGW456L13_3090 at 3445.901 kb on + strand, within PfGW456L13_3090 at 3445.980 kb on - strand, within PfGW456L13_3090 at 3445.980 kb on - strand, within PfGW456L13_3090 at 3446.050 kb on + strand, within PfGW456L13_3090 at 3446.050 kb on + strand, within PfGW456L13_3090 at 3446.051 kb on - strand, within PfGW456L13_3090 at 3446.051 kb on - strand, within PfGW456L13_3090 at 3446.051 kb on - strand, within PfGW456L13_3090 at 3446.051 kb on - strand, within PfGW456L13_3090 at 3446.051 kb on - strand, within PfGW456L13_3090 at 3446.251 kb on + strand, within PfGW456L13_3090 at 3446.251 kb on + strand, within PfGW456L13_3090 at 3446.251 kb on + strand, within PfGW456L13_3090 at 3446.251 kb on + strand, within PfGW456L13_3090 at 3446.251 kb on + strand, within PfGW456L13_3090 at 3446.251 kb on + strand, within PfGW456L13_3090 at 3446.251 kb on + strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.252 kb on - strand, within PfGW456L13_3090 at 3446.489 kb on + strand at 3446.517 kb on + strand at 3446.518 kb on - strand at 3446.535 kb on - strand at 3446.535 kb on - strand at 3446.585 kb on + strand at 3446.585 kb on + strand at 3446.585 kb on + strand at 3446.585 kb on + strand at 3446.586 kb on - strand at 3446.611 kb on + strand at 3446.611 kb on + strand at 3446.620 kb on + strand at 3446.682 kb on + strand at 3446.683 kb on - strand at 3446.683 kb on - strand at 3446.738 kb on - strand at 3446.823 kb on + strand at 3446.954 kb on - strand at 3446.954 kb on - strand at 3447.049 kb on - strand at 3447.049 kb on - strand at 3447.107 kb on + strand, within PfGW456L13_3091 at 3447.107 kb on + strand, within PfGW456L13_3091 at 3447.138 kb on + strand, within PfGW456L13_3091 at 3447.138 kb on + strand, within PfGW456L13_3091 at 3447.138 kb on + strand, within PfGW456L13_3091 at 3447.138 kb on + strand, within PfGW456L13_3091 at 3447.139 kb on - strand, within PfGW456L13_3091 at 3447.139 kb on - strand, within PfGW456L13_3091 at 3447.207 kb on - strand, within PfGW456L13_3091 at 3447.207 kb on - strand, within PfGW456L13_3091 at 3447.207 kb on - strand, within PfGW456L13_3091 at 3447.292 kb on + strand, within PfGW456L13_3091 at 3447.292 kb on + strand, within PfGW456L13_3091 at 3447.292 kb on + strand, within PfGW456L13_3091 at 3447.292 kb on + strand, within PfGW456L13_3091 at 3447.292 kb on + strand, within PfGW456L13_3091 at 3447.292 kb on + strand, within PfGW456L13_3091 at 3447.293 kb on - strand, within PfGW456L13_3091 at 3447.294 kb on + strand, within PfGW456L13_3091 at 3447.294 kb on + strand, within PfGW456L13_3091 at 3447.294 kb on + strand, within PfGW456L13_3091 at 3447.294 kb on + strand, within PfGW456L13_3091 at 3447.295 kb on - strand, within PfGW456L13_3091 at 3447.295 kb on - strand, within PfGW456L13_3091 at 3447.392 kb on - strand, within PfGW456L13_3091 at 3447.392 kb on - strand, within PfGW456L13_3091
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 3,444,395 - +1.0 3,444,395 - PfGW456L13_3089 0.46 -3.0 3,444,424 + PfGW456L13_3089 0.49 +0.9 3,444,424 + PfGW456L13_3089 0.49 +0.2 3,444,424 + PfGW456L13_3089 0.49 +0.2 3,444,425 - PfGW456L13_3089 0.49 -1.0 3,444,425 - PfGW456L13_3089 0.49 -0.0 3,444,425 - PfGW456L13_3089 0.49 +0.7 3,444,641 + PfGW456L13_3089 0.64 -0.4 3,444,641 + PfGW456L13_3089 0.64 -0.3 3,444,641 + PfGW456L13_3089 0.64 +1.3 3,445,057 + -1.0 3,445,058 - -0.2 3,445,159 + +0.6 3,445,159 + +0.3 3,445,161 + -1.2 3,445,161 + +0.1 3,445,161 + +0.2 3,445,161 + -0.3 3,445,161 + +0.3 3,445,162 - +1.3 3,445,162 - +0.2 3,445,162 - +0.4 3,445,162 - -2.6 3,445,198 + -0.2 3,445,306 - +0.1 3,445,311 + -0.2 3,445,528 + PfGW456L13_3090 0.18 +0.4 3,445,528 + PfGW456L13_3090 0.18 -1.6 3,445,528 + PfGW456L13_3090 0.18 -1.1 3,445,529 - PfGW456L13_3090 0.18 +0.3 3,445,575 + PfGW456L13_3090 0.22 +0.2 3,445,575 + PfGW456L13_3090 0.22 +0.4 3,445,576 - PfGW456L13_3090 0.22 -0.0 3,445,591 + PfGW456L13_3090 0.23 +0.5 3,445,591 + PfGW456L13_3090 0.23 +0.1 3,445,592 - PfGW456L13_3090 0.23 -1.5 3,445,612 + PfGW456L13_3090 0.25 -1.0 3,445,612 + PfGW456L13_3090 0.25 +0.1 3,445,652 - PfGW456L13_3090 0.28 -3.0 3,445,652 - PfGW456L13_3090 0.28 -0.9 3,445,901 + PfGW456L13_3090 0.50 -0.3 3,445,980 - PfGW456L13_3090 0.57 -0.7 3,445,980 - PfGW456L13_3090 0.57 -0.8 3,446,050 + PfGW456L13_3090 0.63 +1.1 3,446,050 + PfGW456L13_3090 0.63 -0.6 3,446,051 - PfGW456L13_3090 0.63 -1.4 3,446,051 - PfGW456L13_3090 0.63 +0.3 3,446,051 - PfGW456L13_3090 0.63 -0.7 3,446,051 - PfGW456L13_3090 0.63 -0.4 3,446,051 - PfGW456L13_3090 0.63 -0.7 3,446,251 + PfGW456L13_3090 0.80 -0.3 3,446,251 + PfGW456L13_3090 0.80 -1.8 3,446,251 + PfGW456L13_3090 0.80 -0.3 3,446,251 + PfGW456L13_3090 0.80 +0.7 3,446,251 + PfGW456L13_3090 0.80 -0.7 3,446,251 + PfGW456L13_3090 0.80 +0.4 3,446,251 + PfGW456L13_3090 0.80 -0.6 3,446,252 - PfGW456L13_3090 0.80 +0.1 3,446,252 - PfGW456L13_3090 0.80 +0.6 3,446,252 - PfGW456L13_3090 0.80 -0.0 3,446,252 - PfGW456L13_3090 0.80 -1.7 3,446,252 - PfGW456L13_3090 0.80 -0.2 3,446,252 - PfGW456L13_3090 0.80 -0.5 3,446,252 - PfGW456L13_3090 0.80 +0.4 3,446,252 - PfGW456L13_3090 0.80 -0.3 3,446,489 + -0.3 3,446,517 + -0.2 3,446,518 - +0.2 3,446,535 - +0.4 3,446,535 - +0.3 3,446,585 + -0.0 3,446,585 + -0.5 3,446,585 + +0.7 3,446,585 + -0.2 3,446,586 - +0.5 3,446,611 + -0.2 3,446,611 + -0.8 3,446,620 + -2.0 3,446,682 + -0.6 3,446,683 - -2.0 3,446,683 - -0.3 3,446,738 - -1.6 3,446,823 + -0.0 3,446,954 - -0.3 3,446,954 - -1.6 3,447,049 - +1.4 3,447,049 - +1.0 3,447,107 + PfGW456L13_3091 0.11 -0.0 3,447,107 + PfGW456L13_3091 0.11 -0.6 3,447,138 + PfGW456L13_3091 0.12 +0.1 3,447,138 + PfGW456L13_3091 0.12 -0.8 3,447,138 + PfGW456L13_3091 0.12 +1.4 3,447,138 + PfGW456L13_3091 0.12 +1.3 3,447,139 - PfGW456L13_3091 0.12 +1.0 3,447,139 - PfGW456L13_3091 0.12 +0.7 3,447,207 - PfGW456L13_3091 0.14 -0.4 3,447,207 - PfGW456L13_3091 0.14 -1.0 3,447,207 - PfGW456L13_3091 0.14 -0.2 3,447,292 + PfGW456L13_3091 0.17 +0.5 3,447,292 + PfGW456L13_3091 0.17 -0.0 3,447,292 + PfGW456L13_3091 0.17 +0.7 3,447,292 + PfGW456L13_3091 0.17 +0.7 3,447,292 + PfGW456L13_3091 0.17 +1.1 3,447,292 + PfGW456L13_3091 0.17 -1.3 3,447,293 - PfGW456L13_3091 0.17 +0.1 3,447,294 + PfGW456L13_3091 0.17 +1.9 3,447,294 + PfGW456L13_3091 0.17 -1.0 3,447,294 + PfGW456L13_3091 0.17 -1.0 3,447,294 + PfGW456L13_3091 0.17 +0.5 3,447,295 - PfGW456L13_3091 0.17 +1.3 3,447,295 - PfGW456L13_3091 0.17 +0.7 3,447,392 - PfGW456L13_3091 0.20 +0.3 3,447,392 - PfGW456L13_3091 0.20 +1.0
Or see this region's nucleotide sequence