Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_2873

Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_2872 and PfGW456L13_2873 are separated by 205 nucleotidesPfGW456L13_2873 and PfGW456L13_2874 are separated by 7 nucleotides PfGW456L13_2872 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 3,197,488 to 3,199,443 _2872 PfGW456L13_2873 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 3,199,649 to 3,200,422 _2873 PfGW456L13_2874 - D-beta-hydroxybutyrate permease, at 3,200,430 to 3,201,821 _2874 Position (kb) 3199 3200 3201Strain fitness (log2 ratio) -2 -1 0 1at 3198.695 kb on + strand, within PfGW456L13_2872at 3198.764 kb on + strand, within PfGW456L13_2872at 3198.877 kb on + strand, within PfGW456L13_2872at 3198.877 kb on + strandat 3198.952 kb on - strand, within PfGW456L13_2872at 3199.132 kb on + strand, within PfGW456L13_2872at 3199.163 kb on - strand, within PfGW456L13_2872at 3199.163 kb on - strand, within PfGW456L13_2872at 3199.163 kb on - strand, within PfGW456L13_2872at 3199.163 kb on - strand, within PfGW456L13_2872at 3199.462 kb on + strandat 3199.640 kb on - strandat 3199.640 kb on - strandat 3199.965 kb on + strand, within PfGW456L13_2873at 3199.965 kb on + strand, within PfGW456L13_2873at 3199.965 kb on + strand, within PfGW456L13_2873at 3199.965 kb on + strand, within PfGW456L13_2873at 3199.965 kb on + strand, within PfGW456L13_2873at 3199.966 kb on - strand, within PfGW456L13_2873at 3199.966 kb on - strand, within PfGW456L13_2873at 3199.966 kb on - strand, within PfGW456L13_2873at 3199.966 kb on - strand, within PfGW456L13_2873at 3199.966 kb on - strand, within PfGW456L13_2873at 3200.203 kb on - strand, within PfGW456L13_2873at 3200.426 kb on + strandat 3200.426 kb on + strandat 3200.427 kb on - strandat 3200.427 kb on - strandat 3200.450 kb on - strandat 3200.450 kb on - strandat 3200.495 kb on + strandat 3200.495 kb on + strandat 3200.496 kb on - strandat 3200.496 kb on - strandat 3200.496 kb on - strandat 3200.496 kb on - strandat 3200.496 kb on - strandat 3200.498 kb on + strandat 3200.507 kb on + strandat 3200.507 kb on + strandat 3200.507 kb on + strandat 3200.508 kb on - strandat 3200.515 kb on + strandat 3200.515 kb on + strandat 3200.515 kb on + strandat 3200.515 kb on + strandat 3200.515 kb on + strandat 3200.515 kb on + strandat 3200.516 kb on - strandat 3200.516 kb on - strandat 3200.516 kb on - strandat 3200.837 kb on - strand, within PfGW456L13_2874at 3201.056 kb on - strand, within PfGW456L13_2874at 3201.056 kb on - strand, within PfGW456L13_2874at 3201.187 kb on + strand, within PfGW456L13_2874

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
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3,198,695 + PfGW456L13_2872 0.62 -1.6
3,198,764 + PfGW456L13_2872 0.65 +0.6
3,198,877 + PfGW456L13_2872 0.71 +0.8
3,198,877 + -1.6
3,198,952 - PfGW456L13_2872 0.75 -0.6
3,199,132 + PfGW456L13_2872 0.84 +1.5
3,199,163 - PfGW456L13_2872 0.86 -0.9
3,199,163 - PfGW456L13_2872 0.86 +1.2
3,199,163 - PfGW456L13_2872 0.86 -1.1
3,199,163 - PfGW456L13_2872 0.86 -0.3
3,199,462 + +0.6
3,199,640 - +0.9
3,199,640 - -0.5
3,199,965 + PfGW456L13_2873 0.41 +0.9
3,199,965 + PfGW456L13_2873 0.41 -0.1
3,199,965 + PfGW456L13_2873 0.41 +1.0
3,199,965 + PfGW456L13_2873 0.41 +0.3
3,199,965 + PfGW456L13_2873 0.41 -0.0
3,199,966 - PfGW456L13_2873 0.41 -0.0
3,199,966 - PfGW456L13_2873 0.41 +0.7
3,199,966 - PfGW456L13_2873 0.41 -0.3
3,199,966 - PfGW456L13_2873 0.41 +1.0
3,199,966 - PfGW456L13_2873 0.41 -0.3
3,200,203 - PfGW456L13_2873 0.72 +1.0
3,200,426 + +0.5
3,200,426 + +1.0
3,200,427 - -0.3
3,200,427 - +0.5
3,200,450 - -0.4
3,200,450 - -0.8
3,200,495 + +0.7
3,200,495 + +0.2
3,200,496 - -0.6
3,200,496 - -0.3
3,200,496 - -0.8
3,200,496 - -1.0
3,200,496 - -0.4
3,200,498 + +0.4
3,200,507 + -0.4
3,200,507 + +0.5
3,200,507 + -0.8
3,200,508 - -2.0
3,200,515 + +1.0
3,200,515 + -0.0
3,200,515 + -0.1
3,200,515 + -1.6
3,200,515 + +1.3
3,200,515 + -2.0
3,200,516 - +0.5
3,200,516 - +0.8
3,200,516 - -0.9
3,200,837 - PfGW456L13_2874 0.29 +0.2
3,201,056 - PfGW456L13_2874 0.45 -0.1
3,201,056 - PfGW456L13_2874 0.45 -1.0
3,201,187 + PfGW456L13_2874 0.54 -2.3

Or see this region's nucleotide sequence