Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2872 and PfGW456L13_2873 are separated by 205 nucleotides PfGW456L13_2873 and PfGW456L13_2874 are separated by 7 nucleotides
PfGW456L13_2872 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 3,197,488 to 3,199,443
_2872
PfGW456L13_2873 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 3,199,649 to 3,200,422
_2873
PfGW456L13_2874 - D-beta-hydroxybutyrate permease, at 3,200,430 to 3,201,821
_2874
Position (kb)
3199
3200
3201 Strain fitness (log2 ratio)
-2
-1
0
1 at 3198.695 kb on + strand, within PfGW456L13_2872 at 3198.764 kb on + strand, within PfGW456L13_2872 at 3198.877 kb on + strand, within PfGW456L13_2872 at 3198.877 kb on + strand at 3198.952 kb on - strand, within PfGW456L13_2872 at 3199.132 kb on + strand, within PfGW456L13_2872 at 3199.163 kb on - strand, within PfGW456L13_2872 at 3199.163 kb on - strand, within PfGW456L13_2872 at 3199.163 kb on - strand, within PfGW456L13_2872 at 3199.163 kb on - strand, within PfGW456L13_2872 at 3199.462 kb on + strand at 3199.640 kb on - strand at 3199.640 kb on - strand at 3199.965 kb on + strand, within PfGW456L13_2873 at 3199.965 kb on + strand, within PfGW456L13_2873 at 3199.965 kb on + strand, within PfGW456L13_2873 at 3199.965 kb on + strand, within PfGW456L13_2873 at 3199.965 kb on + strand, within PfGW456L13_2873 at 3199.966 kb on - strand, within PfGW456L13_2873 at 3199.966 kb on - strand, within PfGW456L13_2873 at 3199.966 kb on - strand, within PfGW456L13_2873 at 3199.966 kb on - strand, within PfGW456L13_2873 at 3199.966 kb on - strand, within PfGW456L13_2873 at 3200.203 kb on - strand, within PfGW456L13_2873 at 3200.426 kb on + strand at 3200.426 kb on + strand at 3200.427 kb on - strand at 3200.427 kb on - strand at 3200.450 kb on - strand at 3200.450 kb on - strand at 3200.495 kb on + strand at 3200.495 kb on + strand at 3200.496 kb on - strand at 3200.496 kb on - strand at 3200.496 kb on - strand at 3200.496 kb on - strand at 3200.496 kb on - strand at 3200.498 kb on + strand at 3200.507 kb on + strand at 3200.507 kb on + strand at 3200.507 kb on + strand at 3200.508 kb on - strand at 3200.515 kb on + strand at 3200.515 kb on + strand at 3200.515 kb on + strand at 3200.515 kb on + strand at 3200.515 kb on + strand at 3200.515 kb on + strand at 3200.516 kb on - strand at 3200.516 kb on - strand at 3200.516 kb on - strand at 3200.837 kb on - strand, within PfGW456L13_2874 at 3201.056 kb on - strand, within PfGW456L13_2874 at 3201.056 kb on - strand, within PfGW456L13_2874 at 3201.187 kb on + strand, within PfGW456L13_2874
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 3,198,695 + PfGW456L13_2872 0.62 -1.6 3,198,764 + PfGW456L13_2872 0.65 +0.6 3,198,877 + PfGW456L13_2872 0.71 +0.8 3,198,877 + -1.6 3,198,952 - PfGW456L13_2872 0.75 -0.6 3,199,132 + PfGW456L13_2872 0.84 +1.5 3,199,163 - PfGW456L13_2872 0.86 -0.9 3,199,163 - PfGW456L13_2872 0.86 +1.2 3,199,163 - PfGW456L13_2872 0.86 -1.1 3,199,163 - PfGW456L13_2872 0.86 -0.3 3,199,462 + +0.6 3,199,640 - +0.9 3,199,640 - -0.5 3,199,965 + PfGW456L13_2873 0.41 +0.9 3,199,965 + PfGW456L13_2873 0.41 -0.1 3,199,965 + PfGW456L13_2873 0.41 +1.0 3,199,965 + PfGW456L13_2873 0.41 +0.3 3,199,965 + PfGW456L13_2873 0.41 -0.0 3,199,966 - PfGW456L13_2873 0.41 -0.0 3,199,966 - PfGW456L13_2873 0.41 +0.7 3,199,966 - PfGW456L13_2873 0.41 -0.3 3,199,966 - PfGW456L13_2873 0.41 +1.0 3,199,966 - PfGW456L13_2873 0.41 -0.3 3,200,203 - PfGW456L13_2873 0.72 +1.0 3,200,426 + +0.5 3,200,426 + +1.0 3,200,427 - -0.3 3,200,427 - +0.5 3,200,450 - -0.4 3,200,450 - -0.8 3,200,495 + +0.7 3,200,495 + +0.2 3,200,496 - -0.6 3,200,496 - -0.3 3,200,496 - -0.8 3,200,496 - -1.0 3,200,496 - -0.4 3,200,498 + +0.4 3,200,507 + -0.4 3,200,507 + +0.5 3,200,507 + -0.8 3,200,508 - -2.0 3,200,515 + +1.0 3,200,515 + -0.0 3,200,515 + -0.1 3,200,515 + -1.6 3,200,515 + +1.3 3,200,515 + -2.0 3,200,516 - +0.5 3,200,516 - +0.8 3,200,516 - -0.9 3,200,837 - PfGW456L13_2874 0.29 +0.2 3,201,056 - PfGW456L13_2874 0.45 -0.1 3,201,056 - PfGW456L13_2874 0.45 -1.0 3,201,187 + PfGW456L13_2874 0.54 -2.3
Or see this region's nucleotide sequence