Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2401 and PfGW456L13_2402 are separated by 253 nucleotides PfGW456L13_2402 and PfGW456L13_2403 are separated by 104 nucleotides
PfGW456L13_2401 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconic acid reductase B (EC 1.1.1.274), at 2,664,737 to 2,665,540
_2401
PfGW456L13_2402 - FIG032225: Transcriptional regulator, LysR family, at 2,665,794 to 2,666,702
_2402
PfGW456L13_2403 - Pirin, at 2,666,807 to 2,667,673
_2403
Position (kb)
2665
2666
2667 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2664.993 kb on + strand, within PfGW456L13_2401 at 2665.008 kb on + strand, within PfGW456L13_2401 at 2665.008 kb on + strand, within PfGW456L13_2401 at 2665.008 kb on + strand, within PfGW456L13_2401 at 2665.008 kb on + strand, within PfGW456L13_2401 at 2665.008 kb on + strand, within PfGW456L13_2401 at 2665.008 kb on + strand, within PfGW456L13_2401 at 2665.009 kb on - strand, within PfGW456L13_2401 at 2665.009 kb on - strand, within PfGW456L13_2401 at 2665.069 kb on - strand, within PfGW456L13_2401 at 2665.273 kb on - strand, within PfGW456L13_2401 at 2665.513 kb on + strand at 2665.513 kb on + strand at 2665.514 kb on - strand at 2665.514 kb on - strand at 2665.534 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.717 kb on + strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.718 kb on - strand at 2665.719 kb on + strand at 2665.719 kb on + strand at 2665.719 kb on + strand at 2665.719 kb on + strand at 2665.719 kb on + strand at 2665.719 kb on + strand at 2665.719 kb on + strand at 2665.720 kb on - strand at 2665.720 kb on - strand at 2665.720 kb on - strand at 2665.720 kb on - strand at 2665.720 kb on - strand at 2665.720 kb on - strand at 2665.720 kb on - strand at 2666.099 kb on - strand, within PfGW456L13_2402 at 2666.360 kb on - strand, within PfGW456L13_2402 at 2666.504 kb on - strand, within PfGW456L13_2402 at 2666.504 kb on - strand, within PfGW456L13_2402 at 2666.506 kb on + strand, within PfGW456L13_2402 at 2666.674 kb on + strand at 2666.674 kb on + strand at 2666.674 kb on + strand at 2666.675 kb on - strand at 2666.675 kb on - strand at 2666.677 kb on + strand at 2666.678 kb on - strand at 2666.714 kb on + strand at 2666.742 kb on + strand at 2666.828 kb on + strand at 2666.828 kb on + strand at 2666.829 kb on - strand at 2666.829 kb on - strand at 2666.829 kb on - strand at 2666.829 kb on - strand at 2666.829 kb on - strand at 2666.829 kb on - strand at 2667.023 kb on + strand, within PfGW456L13_2403 at 2667.671 kb on + strand at 2667.672 kb on - strand at 2667.672 kb on - strand at 2667.686 kb on + strand at 2667.687 kb on - strand at 2667.699 kb on + strand at 2667.700 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,664,993 + PfGW456L13_2401 0.32 +0.6 2,665,008 + PfGW456L13_2401 0.34 -0.5 2,665,008 + PfGW456L13_2401 0.34 -0.2 2,665,008 + PfGW456L13_2401 0.34 -1.3 2,665,008 + PfGW456L13_2401 0.34 -0.2 2,665,008 + PfGW456L13_2401 0.34 +0.1 2,665,008 + PfGW456L13_2401 0.34 -0.5 2,665,009 - PfGW456L13_2401 0.34 -0.6 2,665,009 - PfGW456L13_2401 0.34 -1.0 2,665,069 - PfGW456L13_2401 0.41 -0.6 2,665,273 - PfGW456L13_2401 0.67 -0.8 2,665,513 + -1.1 2,665,513 + -1.4 2,665,514 - -1.5 2,665,514 - +0.1 2,665,534 + +1.2 2,665,717 + -0.9 2,665,717 + -0.4 2,665,717 + +0.9 2,665,717 + -0.6 2,665,717 + +0.8 2,665,717 + +1.8 2,665,717 + -0.0 2,665,717 + +0.8 2,665,717 + -0.4 2,665,717 + -0.3 2,665,717 + -2.4 2,665,717 + -2.4 2,665,717 + -2.4 2,665,717 + +2.3 2,665,718 - -1.2 2,665,718 - +0.5 2,665,718 - +0.8 2,665,718 - -0.0 2,665,718 - -0.0 2,665,718 - -0.6 2,665,718 - -1.4 2,665,718 - +0.6 2,665,718 - -1.0 2,665,718 - -1.3 2,665,718 - -1.6 2,665,719 + -0.9 2,665,719 + +0.4 2,665,719 + -1.0 2,665,719 + -1.0 2,665,719 + -1.1 2,665,719 + -0.6 2,665,719 + +2.0 2,665,720 - -0.3 2,665,720 - -0.9 2,665,720 - -0.6 2,665,720 - -2.1 2,665,720 - +0.3 2,665,720 - -1.6 2,665,720 - -2.0 2,666,099 - PfGW456L13_2402 0.34 -0.8 2,666,360 - PfGW456L13_2402 0.62 +0.1 2,666,504 - PfGW456L13_2402 0.78 +0.6 2,666,504 - PfGW456L13_2402 0.78 -1.6 2,666,506 + PfGW456L13_2402 0.78 -1.4 2,666,674 + -1.4 2,666,674 + -0.0 2,666,674 + -0.6 2,666,675 - +0.7 2,666,675 - -0.4 2,666,677 + -1.0 2,666,678 - -0.0 2,666,714 + +0.1 2,666,742 + -0.5 2,666,828 + +3.3 2,666,828 + -1.0 2,666,829 - -0.5 2,666,829 - +0.6 2,666,829 - -0.4 2,666,829 - -0.2 2,666,829 - -0.6 2,666,829 - -0.9 2,667,023 + PfGW456L13_2403 0.25 +0.5 2,667,671 + +0.6 2,667,672 - +1.2 2,667,672 - -1.2 2,667,686 + -0.5 2,667,687 - -1.2 2,667,699 + +0.5 2,667,700 - -1.1
Or see this region's nucleotide sequence