Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2314 and PfGW456L13_2315 are separated by 101 nucleotides PfGW456L13_2315 and PfGW456L13_2316 are separated by 206 nucleotides PfGW456L13_2316 and PfGW456L13_2317 are separated by 65 nucleotides
PfGW456L13_2314 - ABC-type proline/glycine betaine transport systems, ATPase components, at 2,539,266 to 2,539,535
_2314
PfGW456L13_2315 - Transporter, LysE family, at 2,539,637 to 2,540,251
_2315
PfGW456L13_2316 - Cysteine desulfurase (EC 2.8.1.7), at 2,540,458 to 2,541,615
_2316
PfGW456L13_2317 - Xaa-Pro aminopeptidase (EC 3.4.11.9), at 2,541,681 to 2,543,489
_2317
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2539.510 kb on + strand at 2539.511 kb on - strand at 2539.546 kb on + strand at 2539.546 kb on + strand at 2539.547 kb on - strand at 2539.547 kb on - strand at 2539.565 kb on + strand at 2539.565 kb on + strand at 2539.798 kb on - strand, within PfGW456L13_2315 at 2539.844 kb on + strand, within PfGW456L13_2315 at 2539.845 kb on - strand, within PfGW456L13_2315 at 2539.856 kb on + strand, within PfGW456L13_2315 at 2539.856 kb on + strand, within PfGW456L13_2315 at 2539.857 kb on - strand, within PfGW456L13_2315 at 2539.857 kb on - strand, within PfGW456L13_2315 at 2539.871 kb on + strand, within PfGW456L13_2315 at 2539.872 kb on - strand, within PfGW456L13_2315 at 2539.872 kb on - strand, within PfGW456L13_2315 at 2539.872 kb on - strand, within PfGW456L13_2315 at 2539.872 kb on - strand, within PfGW456L13_2315 at 2539.872 kb on - strand, within PfGW456L13_2315 at 2539.886 kb on + strand, within PfGW456L13_2315 at 2539.886 kb on + strand, within PfGW456L13_2315 at 2539.887 kb on - strand, within PfGW456L13_2315 at 2539.887 kb on - strand, within PfGW456L13_2315 at 2539.967 kb on + strand, within PfGW456L13_2315 at 2539.967 kb on + strand, within PfGW456L13_2315 at 2539.967 kb on + strand, within PfGW456L13_2315 at 2539.968 kb on - strand, within PfGW456L13_2315 at 2539.968 kb on - strand, within PfGW456L13_2315 at 2539.968 kb on - strand, within PfGW456L13_2315 at 2539.968 kb on - strand, within PfGW456L13_2315 at 2540.029 kb on + strand, within PfGW456L13_2315 at 2540.029 kb on + strand, within PfGW456L13_2315 at 2540.030 kb on - strand, within PfGW456L13_2315 at 2540.039 kb on + strand, within PfGW456L13_2315 at 2540.039 kb on + strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.040 kb on - strand, within PfGW456L13_2315 at 2540.057 kb on + strand, within PfGW456L13_2315 at 2540.057 kb on + strand, within PfGW456L13_2315 at 2540.057 kb on + strand, within PfGW456L13_2315 at 2540.058 kb on - strand, within PfGW456L13_2315 at 2540.232 kb on + strand at 2540.246 kb on - strand at 2540.284 kb on + strand at 2540.284 kb on + strand at 2540.285 kb on - strand at 2540.285 kb on - strand at 2540.310 kb on - strand at 2540.319 kb on + strand at 2540.320 kb on - strand at 2540.328 kb on + strand at 2540.328 kb on + strand at 2540.328 kb on + strand at 2540.328 kb on + strand at 2540.329 kb on - strand at 2540.329 kb on - strand at 2540.357 kb on + strand at 2540.357 kb on + strand at 2540.357 kb on + strand at 2540.358 kb on - strand at 2540.358 kb on - strand at 2540.363 kb on + strand at 2540.457 kb on + strand at 2540.463 kb on + strand at 2540.463 kb on + strand at 2540.463 kb on + strand at 2540.476 kb on + strand at 2540.476 kb on + strand at 2540.476 kb on + strand at 2540.477 kb on - strand at 2540.477 kb on - strand at 2540.477 kb on - strand at 2540.485 kb on + strand at 2540.486 kb on - strand at 2540.748 kb on + strand, within PfGW456L13_2316 at 2540.749 kb on - strand, within PfGW456L13_2316 at 2540.827 kb on + strand, within PfGW456L13_2316 at 2540.827 kb on + strand, within PfGW456L13_2316 at 2540.827 kb on + strand, within PfGW456L13_2316 at 2540.828 kb on - strand, within PfGW456L13_2316 at 2540.828 kb on - strand, within PfGW456L13_2316 at 2540.828 kb on - strand, within PfGW456L13_2316 at 2540.828 kb on - strand, within PfGW456L13_2316 at 2541.083 kb on - strand, within PfGW456L13_2316 at 2541.109 kb on + strand, within PfGW456L13_2316 at 2541.109 kb on + strand, within PfGW456L13_2316 at 2541.109 kb on + strand, within PfGW456L13_2316 at 2541.110 kb on - strand, within PfGW456L13_2316 at 2541.110 kb on - strand, within PfGW456L13_2316 at 2541.470 kb on + strand, within PfGW456L13_2316 at 2541.470 kb on + strand, within PfGW456L13_2316 at 2541.470 kb on + strand, within PfGW456L13_2316 at 2541.470 kb on + strand, within PfGW456L13_2316 at 2541.470 kb on + strand, within PfGW456L13_2316 at 2541.470 kb on + strand, within PfGW456L13_2316 at 2541.471 kb on - strand, within PfGW456L13_2316 at 2541.471 kb on - strand, within PfGW456L13_2316 at 2541.471 kb on - strand at 2541.471 kb on - strand, within PfGW456L13_2316 at 2541.471 kb on - strand, within PfGW456L13_2316 at 2541.471 kb on - strand, within PfGW456L13_2316 at 2541.471 kb on - strand, within PfGW456L13_2316 at 2541.471 kb on - strand, within PfGW456L13_2316 at 2541.613 kb on + strand at 2541.614 kb on - strand at 2541.652 kb on + strand at 2541.652 kb on + strand at 2541.653 kb on - strand at 2541.653 kb on - strand at 2541.653 kb on - strand at 2541.653 kb on - strand at 2541.686 kb on + strand at 2541.686 kb on + strand at 2541.687 kb on - strand at 2541.804 kb on + strand at 2541.805 kb on - strand at 2541.805 kb on - strand at 2541.805 kb on - strand at 2542.177 kb on - strand, within PfGW456L13_2317 at 2542.455 kb on + strand, within PfGW456L13_2317 at 2542.456 kb on - strand, within PfGW456L13_2317 at 2542.469 kb on + strand, within PfGW456L13_2317
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,539,510 + +1.0 2,539,511 - -0.4 2,539,546 + -0.0 2,539,546 + -0.0 2,539,547 - +1.4 2,539,547 - +1.8 2,539,565 + +2.8 2,539,565 + +0.7 2,539,798 - PfGW456L13_2315 0.26 +0.1 2,539,844 + PfGW456L13_2315 0.34 +0.7 2,539,845 - PfGW456L13_2315 0.34 -0.4 2,539,856 + PfGW456L13_2315 0.36 +0.9 2,539,856 + PfGW456L13_2315 0.36 +0.5 2,539,857 - PfGW456L13_2315 0.36 +0.1 2,539,857 - PfGW456L13_2315 0.36 +0.4 2,539,871 + PfGW456L13_2315 0.38 +1.2 2,539,872 - PfGW456L13_2315 0.38 +0.1 2,539,872 - PfGW456L13_2315 0.38 -0.3 2,539,872 - PfGW456L13_2315 0.38 +1.3 2,539,872 - PfGW456L13_2315 0.38 -2.6 2,539,872 - PfGW456L13_2315 0.38 +0.6 2,539,886 + PfGW456L13_2315 0.40 +0.4 2,539,886 + PfGW456L13_2315 0.40 -2.0 2,539,887 - PfGW456L13_2315 0.41 -1.2 2,539,887 - PfGW456L13_2315 0.41 +0.1 2,539,967 + PfGW456L13_2315 0.54 -0.0 2,539,967 + PfGW456L13_2315 0.54 +0.2 2,539,967 + PfGW456L13_2315 0.54 +1.0 2,539,968 - PfGW456L13_2315 0.54 -1.6 2,539,968 - PfGW456L13_2315 0.54 -0.2 2,539,968 - PfGW456L13_2315 0.54 -0.0 2,539,968 - PfGW456L13_2315 0.54 +0.0 2,540,029 + PfGW456L13_2315 0.64 -0.8 2,540,029 + PfGW456L13_2315 0.64 -0.1 2,540,030 - PfGW456L13_2315 0.64 -0.8 2,540,039 + PfGW456L13_2315 0.65 +0.1 2,540,039 + PfGW456L13_2315 0.65 +0.1 2,540,040 - PfGW456L13_2315 0.66 -0.0 2,540,040 - PfGW456L13_2315 0.66 -0.0 2,540,040 - PfGW456L13_2315 0.66 -2.2 2,540,040 - PfGW456L13_2315 0.66 -0.8 2,540,040 - PfGW456L13_2315 0.66 -0.6 2,540,040 - PfGW456L13_2315 0.66 -0.3 2,540,040 - PfGW456L13_2315 0.66 -0.6 2,540,040 - PfGW456L13_2315 0.66 +1.9 2,540,057 + PfGW456L13_2315 0.68 +0.5 2,540,057 + PfGW456L13_2315 0.68 +1.6 2,540,057 + PfGW456L13_2315 0.68 +0.3 2,540,058 - PfGW456L13_2315 0.68 -1.3 2,540,232 + -1.3 2,540,246 - +0.1 2,540,284 + +0.6 2,540,284 + -0.3 2,540,285 - +0.7 2,540,285 - -0.0 2,540,310 - -1.3 2,540,319 + -0.5 2,540,320 - -1.6 2,540,328 + +1.2 2,540,328 + -1.4 2,540,328 + +1.1 2,540,328 + -2.0 2,540,329 - -0.4 2,540,329 - +0.7 2,540,357 + -0.3 2,540,357 + -0.6 2,540,357 + +0.2 2,540,358 - +1.0 2,540,358 - +0.4 2,540,363 + -1.4 2,540,457 + -0.4 2,540,463 + +2.6 2,540,463 + -0.0 2,540,463 + +1.4 2,540,476 + -0.8 2,540,476 + +0.2 2,540,476 + +0.0 2,540,477 - -0.8 2,540,477 - +0.3 2,540,477 - +0.4 2,540,485 + -0.3 2,540,486 - +0.4 2,540,748 + PfGW456L13_2316 0.25 +1.6 2,540,749 - PfGW456L13_2316 0.25 -0.3 2,540,827 + PfGW456L13_2316 0.32 -0.1 2,540,827 + PfGW456L13_2316 0.32 +1.6 2,540,827 + PfGW456L13_2316 0.32 +0.8 2,540,828 - PfGW456L13_2316 0.32 +0.6 2,540,828 - PfGW456L13_2316 0.32 -0.0 2,540,828 - PfGW456L13_2316 0.32 -1.2 2,540,828 - PfGW456L13_2316 0.32 -0.4 2,541,083 - PfGW456L13_2316 0.54 +0.5 2,541,109 + PfGW456L13_2316 0.56 -1.1 2,541,109 + PfGW456L13_2316 0.56 +2.0 2,541,109 + PfGW456L13_2316 0.56 +0.9 2,541,110 - PfGW456L13_2316 0.56 +0.8 2,541,110 - PfGW456L13_2316 0.56 +0.1 2,541,470 + PfGW456L13_2316 0.87 +1.2 2,541,470 + PfGW456L13_2316 0.87 +1.2 2,541,470 + PfGW456L13_2316 0.87 -0.4 2,541,470 + PfGW456L13_2316 0.87 +0.6 2,541,470 + PfGW456L13_2316 0.87 +0.4 2,541,470 + PfGW456L13_2316 0.87 -0.2 2,541,471 - PfGW456L13_2316 0.87 -0.2 2,541,471 - PfGW456L13_2316 0.87 -1.2 2,541,471 - +0.6 2,541,471 - PfGW456L13_2316 0.87 +2.6 2,541,471 - PfGW456L13_2316 0.87 -1.4 2,541,471 - PfGW456L13_2316 0.87 -0.2 2,541,471 - PfGW456L13_2316 0.87 -0.8 2,541,471 - PfGW456L13_2316 0.87 -0.0 2,541,613 + +0.6 2,541,614 - -0.0 2,541,652 + +1.2 2,541,652 + +0.2 2,541,653 - +0.4 2,541,653 - +0.4 2,541,653 - +1.3 2,541,653 - -0.0 2,541,686 + -0.7 2,541,686 + -1.8 2,541,687 - -1.2 2,541,804 + +1.1 2,541,805 - +1.4 2,541,805 - -2.0 2,541,805 - +0.5 2,542,177 - PfGW456L13_2317 0.27 -0.3 2,542,455 + PfGW456L13_2317 0.43 -0.2 2,542,456 - PfGW456L13_2317 0.43 -0.5 2,542,469 + PfGW456L13_2317 0.44 -1.0
Or see this region's nucleotide sequence