Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1725 and PfGW456L13_1726 are separated by 161 nucleotides PfGW456L13_1726 and PfGW456L13_1727 are separated by 169 nucleotides PfGW456L13_1727 and PfGW456L13_1728 are separated by 64 nucleotides
PfGW456L13_1725 - virulence sensor protein, at 1,900,809 to 1,902,164
_1725
PfGW456L13_1726 - FIG00953680: hypothetical protein, at 1,902,326 to 1,902,613
_1726
PfGW456L13_1727 - NAD(FAD)-utilizing enzyme possibly involved in translation, at 1,902,783 to 1,903,640
_1727
PfGW456L13_1728 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 1,903,705 to 1,904,904
_1728
Position (kb) 1902
1903
1904 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1901.889 kb on + strand, within PfGW456L13_1725 at 1902.008 kb on + strand, within PfGW456L13_1725 at 1902.065 kb on + strand at 1902.065 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.069 kb on + strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.070 kb on - strand at 1902.090 kb on + strand at 1902.090 kb on + strand at 1902.090 kb on + strand at 1902.090 kb on + strand at 1902.091 kb on - strand at 1902.091 kb on - strand at 1902.091 kb on - strand at 1902.091 kb on - strand at 1902.093 kb on - strand at 1902.110 kb on - strand at 1902.239 kb on - strand at 1902.274 kb on + strand at 1902.274 kb on + strand at 1902.274 kb on + strand at 1902.275 kb on - strand at 1902.275 kb on - strand at 1902.275 kb on - strand at 1902.275 kb on - strand at 1902.277 kb on + strand at 1902.277 kb on + strand at 1902.278 kb on - strand at 1902.278 kb on - strand at 1902.278 kb on - strand at 1902.278 kb on - strand at 1902.308 kb on + strand at 1902.308 kb on + strand at 1902.308 kb on + strand at 1902.308 kb on + strand at 1902.308 kb on + strand at 1902.309 kb on - strand at 1902.309 kb on - strand at 1902.334 kb on + strand at 1902.334 kb on + strand at 1902.335 kb on - strand at 1902.335 kb on - strand at 1902.360 kb on + strand, within PfGW456L13_1726 at 1902.368 kb on + strand, within PfGW456L13_1726 at 1902.376 kb on + strand, within PfGW456L13_1726 at 1902.376 kb on + strand, within PfGW456L13_1726 at 1902.377 kb on - strand, within PfGW456L13_1726 at 1902.574 kb on + strand, within PfGW456L13_1726 at 1902.575 kb on - strand, within PfGW456L13_1726 at 1902.661 kb on + strand at 1902.662 kb on - strand at 1902.662 kb on - strand at 1902.696 kb on + strand at 1902.696 kb on + strand at 1902.696 kb on + strand at 1902.696 kb on + strand at 1902.697 kb on - strand at 1902.697 kb on - strand at 1902.708 kb on - strand at 1902.829 kb on + strand at 1902.830 kb on - strand at 1902.876 kb on + strand, within PfGW456L13_1727 at 1902.876 kb on + strand, within PfGW456L13_1727 at 1902.876 kb on + strand, within PfGW456L13_1727 at 1902.877 kb on - strand, within PfGW456L13_1727 at 1902.877 kb on - strand, within PfGW456L13_1727 at 1902.962 kb on - strand, within PfGW456L13_1727 at 1902.973 kb on + strand, within PfGW456L13_1727 at 1902.973 kb on + strand, within PfGW456L13_1727 at 1902.973 kb on + strand, within PfGW456L13_1727 at 1902.973 kb on + strand, within PfGW456L13_1727 at 1902.973 kb on + strand, within PfGW456L13_1727 at 1902.973 kb on + strand, within PfGW456L13_1727 at 1902.974 kb on - strand, within PfGW456L13_1727 at 1902.974 kb on - strand, within PfGW456L13_1727 at 1902.974 kb on - strand, within PfGW456L13_1727 at 1902.974 kb on - strand at 1902.974 kb on - strand, within PfGW456L13_1727 at 1902.974 kb on - strand, within PfGW456L13_1727 at 1902.974 kb on - strand, within PfGW456L13_1727 at 1902.997 kb on + strand, within PfGW456L13_1727 at 1902.998 kb on - strand, within PfGW456L13_1727 at 1902.998 kb on - strand, within PfGW456L13_1727 at 1903.042 kb on + strand, within PfGW456L13_1727 at 1903.042 kb on + strand, within PfGW456L13_1727 at 1903.047 kb on + strand, within PfGW456L13_1727 at 1903.048 kb on - strand, within PfGW456L13_1727 at 1903.048 kb on - strand, within PfGW456L13_1727 at 1903.048 kb on - strand, within PfGW456L13_1727 at 1903.048 kb on - strand, within PfGW456L13_1727 at 1903.048 kb on - strand, within PfGW456L13_1727 at 1903.129 kb on + strand, within PfGW456L13_1727 at 1903.130 kb on - strand, within PfGW456L13_1727 at 1903.130 kb on - strand, within PfGW456L13_1727 at 1903.180 kb on + strand, within PfGW456L13_1727 at 1903.180 kb on + strand, within PfGW456L13_1727 at 1903.181 kb on - strand, within PfGW456L13_1727 at 1903.462 kb on + strand, within PfGW456L13_1727 at 1903.463 kb on - strand, within PfGW456L13_1727 at 1903.706 kb on + strand at 1903.706 kb on + strand at 1903.706 kb on + strand at 1903.706 kb on + strand at 1903.706 kb on + strand at 1903.707 kb on - strand at 1903.707 kb on - strand at 1903.707 kb on - strand at 1903.707 kb on - strand at 1903.707 kb on - strand at 1903.707 kb on - strand at 1903.751 kb on + strand at 1903.751 kb on + strand at 1903.752 kb on - strand at 1903.768 kb on + strand at 1903.910 kb on + strand, within PfGW456L13_1728 at 1903.910 kb on + strand, within PfGW456L13_1728 at 1903.910 kb on + strand, within PfGW456L13_1728 at 1903.911 kb on - strand, within PfGW456L13_1728 at 1903.911 kb on - strand, within PfGW456L13_1728 at 1904.033 kb on + strand, within PfGW456L13_1728 at 1904.033 kb on + strand, within PfGW456L13_1728 at 1904.033 kb on + strand, within PfGW456L13_1728 at 1904.034 kb on - strand, within PfGW456L13_1728 at 1904.034 kb on - strand, within PfGW456L13_1728 at 1904.214 kb on + strand, within PfGW456L13_1728 at 1904.214 kb on + strand, within PfGW456L13_1728 at 1904.214 kb on + strand, within PfGW456L13_1728 at 1904.214 kb on + strand, within PfGW456L13_1728 at 1904.215 kb on - strand, within PfGW456L13_1728 at 1904.215 kb on - strand, within PfGW456L13_1728 at 1904.215 kb on - strand, within PfGW456L13_1728 at 1904.245 kb on + strand, within PfGW456L13_1728 at 1904.245 kb on + strand, within PfGW456L13_1728 at 1904.245 kb on + strand, within PfGW456L13_1728 at 1904.246 kb on - strand, within PfGW456L13_1728 at 1904.246 kb on - strand, within PfGW456L13_1728 at 1904.447 kb on + strand, within PfGW456L13_1728 at 1904.448 kb on - strand, within PfGW456L13_1728 at 1904.448 kb on - strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.623 kb on + strand, within PfGW456L13_1728 at 1904.624 kb on - strand, within PfGW456L13_1728 at 1904.624 kb on - strand, within PfGW456L13_1728 at 1904.624 kb on - strand, within PfGW456L13_1728 at 1904.624 kb on - strand, within PfGW456L13_1728 at 1904.624 kb on - strand, within PfGW456L13_1728
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 1,901,889 + PfGW456L13_1725 0.80 -0.1 1,902,008 + PfGW456L13_1725 0.88 +0.6 1,902,065 + -0.2 1,902,065 + +1.5 1,902,069 + -0.0 1,902,069 + +0.1 1,902,069 + +1.5 1,902,069 + -0.5 1,902,069 + -0.5 1,902,069 + -0.8 1,902,069 + -0.1 1,902,069 + -1.3 1,902,069 + -0.7 1,902,069 + +0.9 1,902,070 - -1.4 1,902,070 - -1.6 1,902,070 - +0.2 1,902,070 - -0.8 1,902,070 - -0.5 1,902,070 - +1.0 1,902,070 - -0.1 1,902,070 - -0.2 1,902,070 - +0.8 1,902,090 + -0.0 1,902,090 + +0.9 1,902,090 + +1.0 1,902,090 + -1.7 1,902,091 - +1.6 1,902,091 - +0.8 1,902,091 - +0.9 1,902,091 - +0.2 1,902,093 - +1.0 1,902,110 - +0.0 1,902,239 - +0.7 1,902,274 + -0.1 1,902,274 + +1.3 1,902,274 + +0.4 1,902,275 - +0.8 1,902,275 - -0.0 1,902,275 - -0.3 1,902,275 - -0.0 1,902,277 + -0.3 1,902,277 + -0.5 1,902,278 - -0.5 1,902,278 - -0.4 1,902,278 - -2.0 1,902,278 - -2.6 1,902,308 + +1.0 1,902,308 + +0.8 1,902,308 + -0.3 1,902,308 + +0.7 1,902,308 + +0.5 1,902,309 - -0.7 1,902,309 - -0.2 1,902,334 + +2.0 1,902,334 + +1.2 1,902,335 - -0.0 1,902,335 - -0.5 1,902,360 + PfGW456L13_1726 0.12 -3.0 1,902,368 + PfGW456L13_1726 0.15 +1.6 1,902,376 + PfGW456L13_1726 0.17 +0.7 1,902,376 + PfGW456L13_1726 0.17 +1.4 1,902,377 - PfGW456L13_1726 0.18 +0.5 1,902,574 + PfGW456L13_1726 0.86 +0.6 1,902,575 - PfGW456L13_1726 0.86 -0.5 1,902,661 + +0.4 1,902,662 - -1.6 1,902,662 - +1.4 1,902,696 + +0.4 1,902,696 + -0.1 1,902,696 + -0.7 1,902,696 + -1.4 1,902,697 - -1.0 1,902,697 - -2.8 1,902,708 - -1.3 1,902,829 + +0.1 1,902,830 - -0.9 1,902,876 + PfGW456L13_1727 0.11 +0.7 1,902,876 + PfGW456L13_1727 0.11 -0.9 1,902,876 + PfGW456L13_1727 0.11 -0.1 1,902,877 - PfGW456L13_1727 0.11 -1.1 1,902,877 - PfGW456L13_1727 0.11 +1.9 1,902,962 - PfGW456L13_1727 0.21 -0.6 1,902,973 + PfGW456L13_1727 0.22 +0.3 1,902,973 + PfGW456L13_1727 0.22 -1.0 1,902,973 + PfGW456L13_1727 0.22 -0.4 1,902,973 + PfGW456L13_1727 0.22 +0.9 1,902,973 + PfGW456L13_1727 0.22 +0.6 1,902,973 + PfGW456L13_1727 0.22 +0.6 1,902,974 - PfGW456L13_1727 0.22 +0.5 1,902,974 - PfGW456L13_1727 0.22 +0.1 1,902,974 - PfGW456L13_1727 0.22 -0.5 1,902,974 - -0.0 1,902,974 - PfGW456L13_1727 0.22 +0.6 1,902,974 - PfGW456L13_1727 0.22 -0.4 1,902,974 - PfGW456L13_1727 0.22 -0.0 1,902,997 + PfGW456L13_1727 0.25 +0.8 1,902,998 - PfGW456L13_1727 0.25 +1.4 1,902,998 - PfGW456L13_1727 0.25 +1.0 1,903,042 + PfGW456L13_1727 0.30 -0.7 1,903,042 + PfGW456L13_1727 0.30 -0.0 1,903,047 + PfGW456L13_1727 0.31 -0.4 1,903,048 - PfGW456L13_1727 0.31 +0.8 1,903,048 - PfGW456L13_1727 0.31 -0.8 1,903,048 - PfGW456L13_1727 0.31 +0.5 1,903,048 - PfGW456L13_1727 0.31 +0.2 1,903,048 - PfGW456L13_1727 0.31 +2.0 1,903,129 + PfGW456L13_1727 0.40 +0.1 1,903,130 - PfGW456L13_1727 0.40 +0.6 1,903,130 - PfGW456L13_1727 0.40 +0.5 1,903,180 + PfGW456L13_1727 0.46 +1.0 1,903,180 + PfGW456L13_1727 0.46 -0.5 1,903,181 - PfGW456L13_1727 0.46 +1.0 1,903,462 + PfGW456L13_1727 0.79 +1.6 1,903,463 - PfGW456L13_1727 0.79 +0.1 1,903,706 + +0.2 1,903,706 + -2.0 1,903,706 + -0.3 1,903,706 + +1.3 1,903,706 + -0.3 1,903,707 - -3.2 1,903,707 - -0.0 1,903,707 - -0.0 1,903,707 - -0.9 1,903,707 - -0.9 1,903,707 - +0.6 1,903,751 + +0.3 1,903,751 + -2.0 1,903,752 - -0.3 1,903,768 + +0.4 1,903,910 + PfGW456L13_1728 0.17 +0.6 1,903,910 + PfGW456L13_1728 0.17 -1.2 1,903,910 + PfGW456L13_1728 0.17 -0.4 1,903,911 - PfGW456L13_1728 0.17 -2.3 1,903,911 - PfGW456L13_1728 0.17 -0.4 1,904,033 + PfGW456L13_1728 0.27 +2.0 1,904,033 + PfGW456L13_1728 0.27 -0.2 1,904,033 + PfGW456L13_1728 0.27 +1.2 1,904,034 - PfGW456L13_1728 0.27 -0.4 1,904,034 - PfGW456L13_1728 0.27 +0.8 1,904,214 + PfGW456L13_1728 0.42 +0.2 1,904,214 + PfGW456L13_1728 0.42 +0.7 1,904,214 + PfGW456L13_1728 0.42 +0.8 1,904,214 + PfGW456L13_1728 0.42 +1.5 1,904,215 - PfGW456L13_1728 0.42 +0.3 1,904,215 - PfGW456L13_1728 0.42 -0.6 1,904,215 - PfGW456L13_1728 0.42 +0.1 1,904,245 + PfGW456L13_1728 0.45 +0.1 1,904,245 + PfGW456L13_1728 0.45 +1.2 1,904,245 + PfGW456L13_1728 0.45 +1.0 1,904,246 - PfGW456L13_1728 0.45 +0.3 1,904,246 - PfGW456L13_1728 0.45 -0.5 1,904,447 + PfGW456L13_1728 0.62 +0.7 1,904,448 - PfGW456L13_1728 0.62 +1.4 1,904,448 - PfGW456L13_1728 0.62 +0.3 1,904,623 + PfGW456L13_1728 0.77 +0.2 1,904,623 + PfGW456L13_1728 0.77 -0.4 1,904,623 + PfGW456L13_1728 0.77 -1.0 1,904,623 + PfGW456L13_1728 0.77 -1.3 1,904,623 + PfGW456L13_1728 0.77 -1.0 1,904,623 + PfGW456L13_1728 0.77 -0.0 1,904,623 + PfGW456L13_1728 0.77 -1.0 1,904,623 + PfGW456L13_1728 0.77 -0.3 1,904,624 - PfGW456L13_1728 0.77 +0.4 1,904,624 - PfGW456L13_1728 0.77 +0.6 1,904,624 - PfGW456L13_1728 0.77 -0.3 1,904,624 - PfGW456L13_1728 0.77 -1.4 1,904,624 - PfGW456L13_1728 0.77 -0.6
Or see this region's nucleotide sequence