Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1297 and PfGW456L13_1298 are separated by 354 nucleotides PfGW456L13_1298 and PfGW456L13_1299 are separated by 152 nucleotides
PfGW456L13_1297 - Putative drug efflux protein, at 1,435,365 to 1,436,576
_1297
PfGW456L13_1298 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 1,436,931 to 1,439,060
_1298
PfGW456L13_1299 - Lhr-like helicases, at 1,439,213 to 1,443,520
_1299
Position (kb) 1436
1437
1438
1439
1440 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.961 kb on - strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.048 kb on - strand, within PfGW456L13_1297 at 1436.048 kb on - strand, within PfGW456L13_1297 at 1436.048 kb on - strand, within PfGW456L13_1297 at 1436.220 kb on + strand, within PfGW456L13_1297 at 1436.220 kb on + strand, within PfGW456L13_1297 at 1436.221 kb on - strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.306 kb on - strand, within PfGW456L13_1297 at 1436.437 kb on + strand, within PfGW456L13_1297 at 1436.437 kb on + strand, within PfGW456L13_1297 at 1436.437 kb on + strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.641 kb on + strand at 1436.679 kb on + strand at 1436.680 kb on - strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.785 kb on - strand at 1436.854 kb on + strand at 1436.854 kb on + strand at 1436.854 kb on + strand at 1436.854 kb on + strand at 1436.855 kb on - strand at 1436.855 kb on - strand at 1436.855 kb on - strand at 1436.953 kb on + strand at 1436.954 kb on - strand at 1437.203 kb on + strand, within PfGW456L13_1298 at 1437.372 kb on + strand, within PfGW456L13_1298 at 1437.373 kb on - strand, within PfGW456L13_1298 at 1437.384 kb on + strand, within PfGW456L13_1298 at 1437.384 kb on + strand, within PfGW456L13_1298 at 1437.384 kb on + strand, within PfGW456L13_1298 at 1437.423 kb on + strand, within PfGW456L13_1298 at 1437.423 kb on + strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.480 kb on + strand, within PfGW456L13_1298 at 1437.480 kb on + strand, within PfGW456L13_1298 at 1437.481 kb on - strand, within PfGW456L13_1298 at 1437.481 kb on - strand, within PfGW456L13_1298 at 1437.572 kb on + strand, within PfGW456L13_1298 at 1437.572 kb on + strand, within PfGW456L13_1298 at 1437.672 kb on - strand, within PfGW456L13_1298 at 1437.672 kb on - strand, within PfGW456L13_1298 at 1438.035 kb on + strand, within PfGW456L13_1298 at 1438.299 kb on - strand, within PfGW456L13_1298 at 1438.577 kb on + strand, within PfGW456L13_1298 at 1438.647 kb on - strand, within PfGW456L13_1298 at 1438.695 kb on + strand, within PfGW456L13_1298 at 1438.696 kb on - strand, within PfGW456L13_1298 at 1438.696 kb on - strand, within PfGW456L13_1298 at 1438.696 kb on - strand, within PfGW456L13_1298 at 1438.773 kb on + strand, within PfGW456L13_1298 at 1438.774 kb on - strand, within PfGW456L13_1298 at 1438.919 kb on + strand at 1438.950 kb on + strand at 1439.059 kb on - strand at 1439.059 kb on - strand at 1439.070 kb on + strand at 1439.152 kb on + strand at 1439.214 kb on + strand at 1439.214 kb on + strand at 1439.215 kb on - strand at 1439.215 kb on - strand at 1439.215 kb on - strand at 1439.224 kb on + strand at 1439.225 kb on - strand at 1439.230 kb on + strand at 1439.230 kb on + strand at 1439.231 kb on - strand at 1439.231 kb on - strand at 1439.322 kb on + strand at 1439.323 kb on - strand at 1439.323 kb on - strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.324 kb on + strand at 1439.325 kb on - strand at 1439.325 kb on - strand at 1439.325 kb on - strand at 1439.325 kb on - strand at 1439.325 kb on - strand at 1439.551 kb on + strand at 1439.551 kb on + strand at 1439.552 kb on - strand at 1439.740 kb on + strand, within PfGW456L13_1299 at 1439.749 kb on + strand, within PfGW456L13_1299 at 1439.750 kb on - strand, within PfGW456L13_1299 at 1439.750 kb on - strand, within PfGW456L13_1299 at 1439.750 kb on - strand, within PfGW456L13_1299
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 1,435,960 + PfGW456L13_1297 0.49 +0.1 1,435,960 + PfGW456L13_1297 0.49 +0.1 1,435,960 + PfGW456L13_1297 0.49 +1.1 1,435,960 + PfGW456L13_1297 0.49 -0.3 1,435,960 + PfGW456L13_1297 0.49 -0.0 1,435,960 + PfGW456L13_1297 0.49 -1.1 1,435,960 + PfGW456L13_1297 0.49 -0.1 1,435,960 + PfGW456L13_1297 0.49 +0.5 1,435,961 - PfGW456L13_1297 0.49 -0.1 1,436,047 + PfGW456L13_1297 0.56 +0.1 1,436,047 + PfGW456L13_1297 0.56 +0.4 1,436,047 + PfGW456L13_1297 0.56 -0.0 1,436,047 + PfGW456L13_1297 0.56 +0.2 1,436,048 - PfGW456L13_1297 0.56 -0.2 1,436,048 - PfGW456L13_1297 0.56 +1.4 1,436,048 - PfGW456L13_1297 0.56 -0.0 1,436,220 + PfGW456L13_1297 0.71 +0.4 1,436,220 + PfGW456L13_1297 0.71 -3.3 1,436,221 - PfGW456L13_1297 0.71 +1.8 1,436,305 + PfGW456L13_1297 0.78 -0.3 1,436,305 + PfGW456L13_1297 0.78 -1.5 1,436,305 + PfGW456L13_1297 0.78 -1.3 1,436,305 + PfGW456L13_1297 0.78 -0.1 1,436,306 - PfGW456L13_1297 0.78 +1.0 1,436,437 + PfGW456L13_1297 0.88 -0.5 1,436,437 + PfGW456L13_1297 0.88 -1.3 1,436,437 + PfGW456L13_1297 0.88 +0.7 1,436,438 - PfGW456L13_1297 0.89 -0.4 1,436,438 - PfGW456L13_1297 0.89 -1.0 1,436,438 - PfGW456L13_1297 0.89 +1.2 1,436,438 - PfGW456L13_1297 0.89 +0.7 1,436,641 + +0.5 1,436,679 + +2.3 1,436,680 - +0.7 1,436,698 + -1.4 1,436,698 + -0.8 1,436,698 + -1.5 1,436,698 + +1.1 1,436,698 + +0.1 1,436,698 + +0.1 1,436,698 + -1.2 1,436,698 + +0.3 1,436,698 + -0.3 1,436,699 - +1.4 1,436,699 - -1.1 1,436,699 - -0.5 1,436,699 - +1.9 1,436,699 - +0.8 1,436,699 - -0.2 1,436,699 - -0.3 1,436,699 - +1.6 1,436,699 - -1.6 1,436,699 - +0.4 1,436,699 - +1.8 1,436,699 - +0.5 1,436,699 - -1.3 1,436,699 - -0.1 1,436,699 - -0.7 1,436,785 - +0.4 1,436,854 + -0.8 1,436,854 + -0.4 1,436,854 + -0.3 1,436,854 + -0.1 1,436,855 - -1.8 1,436,855 - +0.4 1,436,855 - -1.2 1,436,953 + +1.9 1,436,954 - -1.3 1,437,203 + PfGW456L13_1298 0.13 +0.1 1,437,372 + PfGW456L13_1298 0.21 +0.5 1,437,373 - PfGW456L13_1298 0.21 -0.3 1,437,384 + PfGW456L13_1298 0.21 +1.1 1,437,384 + PfGW456L13_1298 0.21 +0.8 1,437,384 + PfGW456L13_1298 0.21 -0.0 1,437,423 + PfGW456L13_1298 0.23 +0.1 1,437,423 + PfGW456L13_1298 0.23 +1.2 1,437,424 - PfGW456L13_1298 0.23 +0.1 1,437,424 - PfGW456L13_1298 0.23 -1.0 1,437,424 - PfGW456L13_1298 0.23 -0.4 1,437,424 - PfGW456L13_1298 0.23 +0.7 1,437,424 - PfGW456L13_1298 0.23 -0.0 1,437,424 - PfGW456L13_1298 0.23 +0.8 1,437,480 + PfGW456L13_1298 0.26 +0.2 1,437,480 + PfGW456L13_1298 0.26 +0.8 1,437,481 - PfGW456L13_1298 0.26 +1.4 1,437,481 - PfGW456L13_1298 0.26 +0.5 1,437,572 + PfGW456L13_1298 0.30 +0.5 1,437,572 + PfGW456L13_1298 0.30 -0.6 1,437,672 - PfGW456L13_1298 0.35 +0.1 1,437,672 - PfGW456L13_1298 0.35 -0.5 1,438,035 + PfGW456L13_1298 0.52 +0.5 1,438,299 - PfGW456L13_1298 0.64 -1.8 1,438,577 + PfGW456L13_1298 0.77 +0.8 1,438,647 - PfGW456L13_1298 0.81 +0.6 1,438,695 + PfGW456L13_1298 0.83 -0.0 1,438,696 - PfGW456L13_1298 0.83 +0.9 1,438,696 - PfGW456L13_1298 0.83 +0.8 1,438,696 - PfGW456L13_1298 0.83 +1.4 1,438,773 + PfGW456L13_1298 0.86 +0.3 1,438,774 - PfGW456L13_1298 0.87 +0.9 1,438,919 + -0.6 1,438,950 + -1.2 1,439,059 - -1.4 1,439,059 - -0.3 1,439,070 + +0.3 1,439,152 + -1.0 1,439,214 + -2.0 1,439,214 + -1.8 1,439,215 - -0.0 1,439,215 - -0.2 1,439,215 - +1.1 1,439,224 + +0.6 1,439,225 - -0.3 1,439,230 + +0.2 1,439,230 + +1.9 1,439,231 - +0.3 1,439,231 - +0.1 1,439,322 + -0.7 1,439,323 - -1.7 1,439,323 - +1.6 1,439,324 + -0.3 1,439,324 + -0.9 1,439,324 + -1.1 1,439,324 + +0.9 1,439,324 + -0.1 1,439,324 + -0.4 1,439,324 + -0.2 1,439,324 + +0.5 1,439,324 + +0.3 1,439,325 - -0.0 1,439,325 - +1.3 1,439,325 - +0.7 1,439,325 - -2.8 1,439,325 - -0.7 1,439,551 + +0.7 1,439,551 + +0.2 1,439,552 - -0.7 1,439,740 + PfGW456L13_1299 0.12 -0.9 1,439,749 + PfGW456L13_1299 0.12 -0.0 1,439,750 - PfGW456L13_1299 0.12 -0.3 1,439,750 - PfGW456L13_1299 0.12 -0.0 1,439,750 - PfGW456L13_1299 0.12 -0.3
Or see this region's nucleotide sequence