Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1295 and PfGW456L13_1296 overlap by 4 nucleotides PfGW456L13_1296 and PfGW456L13_1297 are separated by 203 nucleotides PfGW456L13_1297 and PfGW456L13_1298 are separated by 354 nucleotides
PfGW456L13_1295 - Endonuclease/Exonuclease/phosphatase family protein, at 1,433,765 to 1,434,562
_1295
PfGW456L13_1296 - FIG003003: hypothetical protein, at 1,434,559 to 1,435,161
_1296
PfGW456L13_1297 - Putative drug efflux protein, at 1,435,365 to 1,436,576
_1297
PfGW456L13_1298 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 1,436,931 to 1,439,060
_1298
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1434.405 kb on - strand, within PfGW456L13_1295 at 1434.405 kb on - strand, within PfGW456L13_1295 at 1434.405 kb on - strand, within PfGW456L13_1295 at 1434.425 kb on + strand, within PfGW456L13_1295 at 1434.425 kb on + strand, within PfGW456L13_1295 at 1434.425 kb on + strand, within PfGW456L13_1295 at 1434.425 kb on + strand, within PfGW456L13_1295 at 1434.425 kb on + strand, within PfGW456L13_1295 at 1434.425 kb on + strand, within PfGW456L13_1295 at 1434.425 kb on + strand, within PfGW456L13_1295 at 1434.426 kb on - strand, within PfGW456L13_1295 at 1434.426 kb on - strand, within PfGW456L13_1295 at 1434.426 kb on - strand, within PfGW456L13_1295 at 1434.683 kb on + strand, within PfGW456L13_1296 at 1434.683 kb on + strand, within PfGW456L13_1296 at 1434.683 kb on + strand, within PfGW456L13_1296 at 1434.683 kb on + strand, within PfGW456L13_1296 at 1434.684 kb on - strand, within PfGW456L13_1296 at 1434.799 kb on + strand, within PfGW456L13_1296 at 1434.799 kb on + strand, within PfGW456L13_1296 at 1434.800 kb on - strand, within PfGW456L13_1296 at 1434.815 kb on + strand, within PfGW456L13_1296 at 1434.827 kb on + strand, within PfGW456L13_1296 at 1434.827 kb on + strand, within PfGW456L13_1296 at 1434.827 kb on + strand, within PfGW456L13_1296 at 1434.828 kb on - strand, within PfGW456L13_1296 at 1434.828 kb on - strand, within PfGW456L13_1296 at 1434.858 kb on - strand, within PfGW456L13_1296 at 1434.858 kb on - strand, within PfGW456L13_1296 at 1434.897 kb on + strand, within PfGW456L13_1296 at 1434.897 kb on + strand, within PfGW456L13_1296 at 1434.897 kb on + strand, within PfGW456L13_1296 at 1434.897 kb on + strand, within PfGW456L13_1296 at 1434.897 kb on + strand, within PfGW456L13_1296 at 1434.898 kb on - strand, within PfGW456L13_1296 at 1434.898 kb on - strand, within PfGW456L13_1296 at 1434.898 kb on - strand, within PfGW456L13_1296 at 1434.932 kb on + strand, within PfGW456L13_1296 at 1434.933 kb on - strand, within PfGW456L13_1296 at 1434.933 kb on - strand, within PfGW456L13_1296 at 1434.995 kb on + strand, within PfGW456L13_1296 at 1434.995 kb on + strand, within PfGW456L13_1296 at 1435.019 kb on + strand, within PfGW456L13_1296 at 1435.019 kb on + strand, within PfGW456L13_1296 at 1435.019 kb on + strand, within PfGW456L13_1296 at 1435.020 kb on - strand, within PfGW456L13_1296 at 1435.100 kb on + strand, within PfGW456L13_1296 at 1435.101 kb on - strand at 1435.101 kb on - strand at 1435.101 kb on - strand at 1435.124 kb on + strand at 1435.124 kb on + strand at 1435.125 kb on - strand at 1435.125 kb on - strand at 1435.140 kb on + strand at 1435.175 kb on + strand at 1435.175 kb on + strand at 1435.176 kb on - strand at 1435.176 kb on - strand at 1435.301 kb on - strand at 1435.376 kb on + strand at 1435.377 kb on - strand at 1435.377 kb on - strand at 1435.377 kb on - strand at 1435.377 kb on - strand at 1435.411 kb on + strand at 1435.411 kb on + strand at 1435.412 kb on - strand at 1435.412 kb on - strand at 1435.412 kb on - strand at 1435.412 kb on - strand at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.636 kb on + strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.637 kb on - strand, within PfGW456L13_1297 at 1435.732 kb on - strand, within PfGW456L13_1297 at 1435.916 kb on + strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.917 kb on - strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.960 kb on + strand, within PfGW456L13_1297 at 1435.961 kb on - strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.047 kb on + strand, within PfGW456L13_1297 at 1436.048 kb on - strand, within PfGW456L13_1297 at 1436.048 kb on - strand, within PfGW456L13_1297 at 1436.048 kb on - strand, within PfGW456L13_1297 at 1436.220 kb on + strand, within PfGW456L13_1297 at 1436.220 kb on + strand, within PfGW456L13_1297 at 1436.221 kb on - strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.305 kb on + strand, within PfGW456L13_1297 at 1436.306 kb on - strand, within PfGW456L13_1297 at 1436.437 kb on + strand, within PfGW456L13_1297 at 1436.437 kb on + strand, within PfGW456L13_1297 at 1436.437 kb on + strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.438 kb on - strand, within PfGW456L13_1297 at 1436.641 kb on + strand at 1436.679 kb on + strand at 1436.680 kb on - strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.698 kb on + strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.699 kb on - strand at 1436.785 kb on - strand at 1436.854 kb on + strand at 1436.854 kb on + strand at 1436.854 kb on + strand at 1436.854 kb on + strand at 1436.855 kb on - strand at 1436.855 kb on - strand at 1436.855 kb on - strand at 1436.953 kb on + strand at 1436.954 kb on - strand at 1437.203 kb on + strand, within PfGW456L13_1298 at 1437.372 kb on + strand, within PfGW456L13_1298 at 1437.373 kb on - strand, within PfGW456L13_1298 at 1437.384 kb on + strand, within PfGW456L13_1298 at 1437.384 kb on + strand, within PfGW456L13_1298 at 1437.384 kb on + strand, within PfGW456L13_1298 at 1437.423 kb on + strand, within PfGW456L13_1298 at 1437.423 kb on + strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.424 kb on - strand, within PfGW456L13_1298 at 1437.480 kb on + strand, within PfGW456L13_1298 at 1437.480 kb on + strand, within PfGW456L13_1298 at 1437.481 kb on - strand, within PfGW456L13_1298 at 1437.481 kb on - strand, within PfGW456L13_1298 at 1437.572 kb on + strand, within PfGW456L13_1298 at 1437.572 kb on + strand, within PfGW456L13_1298
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 1,434,405 - PfGW456L13_1295 0.80 -0.2 1,434,405 - PfGW456L13_1295 0.80 -0.7 1,434,405 - PfGW456L13_1295 0.80 +0.3 1,434,425 + PfGW456L13_1295 0.83 -0.0 1,434,425 + PfGW456L13_1295 0.83 -2.5 1,434,425 + PfGW456L13_1295 0.83 -0.4 1,434,425 + PfGW456L13_1295 0.83 -1.7 1,434,425 + PfGW456L13_1295 0.83 -0.8 1,434,425 + PfGW456L13_1295 0.83 -0.3 1,434,425 + PfGW456L13_1295 0.83 -0.7 1,434,426 - PfGW456L13_1295 0.83 +0.6 1,434,426 - PfGW456L13_1295 0.83 -0.5 1,434,426 - PfGW456L13_1295 0.83 -1.7 1,434,683 + PfGW456L13_1296 0.21 +1.3 1,434,683 + PfGW456L13_1296 0.21 +1.4 1,434,683 + PfGW456L13_1296 0.21 +0.3 1,434,683 + PfGW456L13_1296 0.21 -0.3 1,434,684 - PfGW456L13_1296 0.21 +0.8 1,434,799 + PfGW456L13_1296 0.40 +1.7 1,434,799 + PfGW456L13_1296 0.40 +0.7 1,434,800 - PfGW456L13_1296 0.40 -1.0 1,434,815 + PfGW456L13_1296 0.42 -0.6 1,434,827 + PfGW456L13_1296 0.44 +0.2 1,434,827 + PfGW456L13_1296 0.44 -0.5 1,434,827 + PfGW456L13_1296 0.44 -0.0 1,434,828 - PfGW456L13_1296 0.45 +0.2 1,434,828 - PfGW456L13_1296 0.45 -0.3 1,434,858 - PfGW456L13_1296 0.50 -0.4 1,434,858 - PfGW456L13_1296 0.50 -0.0 1,434,897 + PfGW456L13_1296 0.56 +1.6 1,434,897 + PfGW456L13_1296 0.56 +1.1 1,434,897 + PfGW456L13_1296 0.56 -0.4 1,434,897 + PfGW456L13_1296 0.56 +0.2 1,434,897 + PfGW456L13_1296 0.56 -0.5 1,434,898 - PfGW456L13_1296 0.56 -0.8 1,434,898 - PfGW456L13_1296 0.56 +0.1 1,434,898 - PfGW456L13_1296 0.56 -0.3 1,434,932 + PfGW456L13_1296 0.62 -0.4 1,434,933 - PfGW456L13_1296 0.62 -1.0 1,434,933 - PfGW456L13_1296 0.62 -0.1 1,434,995 + PfGW456L13_1296 0.72 +0.7 1,434,995 + PfGW456L13_1296 0.72 +0.1 1,435,019 + PfGW456L13_1296 0.76 -0.8 1,435,019 + PfGW456L13_1296 0.76 +0.1 1,435,019 + PfGW456L13_1296 0.76 -0.6 1,435,020 - PfGW456L13_1296 0.76 +0.5 1,435,100 + PfGW456L13_1296 0.90 -2.6 1,435,101 - -0.0 1,435,101 - +0.7 1,435,101 - +0.8 1,435,124 + -0.4 1,435,124 + +1.0 1,435,125 - -1.6 1,435,125 - -0.0 1,435,140 + +1.0 1,435,175 + +2.6 1,435,175 + +1.3 1,435,176 - -0.1 1,435,176 - -0.5 1,435,301 - -3.0 1,435,376 + -1.6 1,435,377 - -1.7 1,435,377 - -0.3 1,435,377 - -0.8 1,435,377 - +0.6 1,435,411 + -1.6 1,435,411 + +0.4 1,435,412 - +0.2 1,435,412 - +0.1 1,435,412 - +0.1 1,435,412 - -0.1 1,435,636 + PfGW456L13_1297 0.22 -1.8 1,435,636 + PfGW456L13_1297 0.22 -0.4 1,435,636 + PfGW456L13_1297 0.22 +0.3 1,435,636 + PfGW456L13_1297 0.22 -0.8 1,435,636 + PfGW456L13_1297 0.22 +0.4 1,435,636 + PfGW456L13_1297 0.22 -0.4 1,435,636 + PfGW456L13_1297 0.22 -1.5 1,435,636 + PfGW456L13_1297 0.22 +0.7 1,435,636 + PfGW456L13_1297 0.22 +0.3 1,435,636 + PfGW456L13_1297 0.22 +0.7 1,435,636 + PfGW456L13_1297 0.22 +0.3 1,435,636 + PfGW456L13_1297 0.22 -1.7 1,435,636 + PfGW456L13_1297 0.22 +0.4 1,435,636 + PfGW456L13_1297 0.22 -0.2 1,435,636 + PfGW456L13_1297 0.22 -0.8 1,435,636 + PfGW456L13_1297 0.22 +0.4 1,435,636 + PfGW456L13_1297 0.22 +0.3 1,435,637 - PfGW456L13_1297 0.22 +0.7 1,435,637 - PfGW456L13_1297 0.22 -1.2 1,435,637 - PfGW456L13_1297 0.22 +0.2 1,435,637 - PfGW456L13_1297 0.22 -1.1 1,435,637 - PfGW456L13_1297 0.22 -3.0 1,435,637 - PfGW456L13_1297 0.22 -0.2 1,435,637 - PfGW456L13_1297 0.22 -0.4 1,435,637 - PfGW456L13_1297 0.22 +0.1 1,435,637 - PfGW456L13_1297 0.22 -0.7 1,435,637 - PfGW456L13_1297 0.22 +0.3 1,435,732 - PfGW456L13_1297 0.30 +0.1 1,435,916 + PfGW456L13_1297 0.45 -1.7 1,435,917 - PfGW456L13_1297 0.46 -0.5 1,435,917 - PfGW456L13_1297 0.46 -1.0 1,435,917 - PfGW456L13_1297 0.46 +0.7 1,435,917 - PfGW456L13_1297 0.46 -0.6 1,435,917 - PfGW456L13_1297 0.46 +0.3 1,435,917 - PfGW456L13_1297 0.46 +1.5 1,435,917 - PfGW456L13_1297 0.46 -0.3 1,435,917 - PfGW456L13_1297 0.46 -0.6 1,435,917 - PfGW456L13_1297 0.46 +0.4 1,435,960 + PfGW456L13_1297 0.49 +0.1 1,435,960 + PfGW456L13_1297 0.49 +0.1 1,435,960 + PfGW456L13_1297 0.49 +1.1 1,435,960 + PfGW456L13_1297 0.49 -0.3 1,435,960 + PfGW456L13_1297 0.49 -0.0 1,435,960 + PfGW456L13_1297 0.49 -1.1 1,435,960 + PfGW456L13_1297 0.49 -0.1 1,435,960 + PfGW456L13_1297 0.49 +0.5 1,435,961 - PfGW456L13_1297 0.49 -0.1 1,436,047 + PfGW456L13_1297 0.56 +0.1 1,436,047 + PfGW456L13_1297 0.56 +0.4 1,436,047 + PfGW456L13_1297 0.56 -0.0 1,436,047 + PfGW456L13_1297 0.56 +0.2 1,436,048 - PfGW456L13_1297 0.56 -0.2 1,436,048 - PfGW456L13_1297 0.56 +1.4 1,436,048 - PfGW456L13_1297 0.56 -0.0 1,436,220 + PfGW456L13_1297 0.71 +0.4 1,436,220 + PfGW456L13_1297 0.71 -3.3 1,436,221 - PfGW456L13_1297 0.71 +1.8 1,436,305 + PfGW456L13_1297 0.78 -0.3 1,436,305 + PfGW456L13_1297 0.78 -1.5 1,436,305 + PfGW456L13_1297 0.78 -1.3 1,436,305 + PfGW456L13_1297 0.78 -0.1 1,436,306 - PfGW456L13_1297 0.78 +1.0 1,436,437 + PfGW456L13_1297 0.88 -0.5 1,436,437 + PfGW456L13_1297 0.88 -1.3 1,436,437 + PfGW456L13_1297 0.88 +0.7 1,436,438 - PfGW456L13_1297 0.89 -0.4 1,436,438 - PfGW456L13_1297 0.89 -1.0 1,436,438 - PfGW456L13_1297 0.89 +1.2 1,436,438 - PfGW456L13_1297 0.89 +0.7 1,436,641 + +0.5 1,436,679 + +2.3 1,436,680 - +0.7 1,436,698 + -1.4 1,436,698 + -0.8 1,436,698 + -1.5 1,436,698 + +1.1 1,436,698 + +0.1 1,436,698 + +0.1 1,436,698 + -1.2 1,436,698 + +0.3 1,436,698 + -0.3 1,436,699 - +1.4 1,436,699 - -1.1 1,436,699 - -0.5 1,436,699 - +1.9 1,436,699 - +0.8 1,436,699 - -0.2 1,436,699 - -0.3 1,436,699 - +1.6 1,436,699 - -1.6 1,436,699 - +0.4 1,436,699 - +1.8 1,436,699 - +0.5 1,436,699 - -1.3 1,436,699 - -0.1 1,436,699 - -0.7 1,436,785 - +0.4 1,436,854 + -0.8 1,436,854 + -0.4 1,436,854 + -0.3 1,436,854 + -0.1 1,436,855 - -1.8 1,436,855 - +0.4 1,436,855 - -1.2 1,436,953 + +1.9 1,436,954 - -1.3 1,437,203 + PfGW456L13_1298 0.13 +0.1 1,437,372 + PfGW456L13_1298 0.21 +0.5 1,437,373 - PfGW456L13_1298 0.21 -0.3 1,437,384 + PfGW456L13_1298 0.21 +1.1 1,437,384 + PfGW456L13_1298 0.21 +0.8 1,437,384 + PfGW456L13_1298 0.21 -0.0 1,437,423 + PfGW456L13_1298 0.23 +0.1 1,437,423 + PfGW456L13_1298 0.23 +1.2 1,437,424 - PfGW456L13_1298 0.23 +0.1 1,437,424 - PfGW456L13_1298 0.23 -1.0 1,437,424 - PfGW456L13_1298 0.23 -0.4 1,437,424 - PfGW456L13_1298 0.23 +0.7 1,437,424 - PfGW456L13_1298 0.23 -0.0 1,437,424 - PfGW456L13_1298 0.23 +0.8 1,437,480 + PfGW456L13_1298 0.26 +0.2 1,437,480 + PfGW456L13_1298 0.26 +0.8 1,437,481 - PfGW456L13_1298 0.26 +1.4 1,437,481 - PfGW456L13_1298 0.26 +0.5 1,437,572 + PfGW456L13_1298 0.30 +0.5 1,437,572 + PfGW456L13_1298 0.30 -0.6
Or see this region's nucleotide sequence