Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF880 and GFF881 are separated by 174 nucleotides GFF881 and GFF882 overlap by 4 nucleotides GFF882 and GFF883 are separated by 233 nucleotides
GFF880 - Fructose-1,6-bisphosphatase, type I (EC 3.1.3.11), at 58,847 to 59,845
GFF880
GFF881 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-), at 60,020 to 61,399
GFF881
GFF882 - FIG01048950: hypothetical protein, at 61,396 to 61,656
GFF882
GFF883 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 61,890 to 62,723
GFF883
Position (kb) 60
61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 59.077 kb on - strand, within GFF880 at 59.130 kb on + strand, within GFF880 at 59.142 kb on + strand, within GFF880 at 59.217 kb on + strand, within GFF880 at 59.222 kb on - strand, within GFF880 at 59.266 kb on + strand, within GFF880 at 59.266 kb on + strand, within GFF880 at 59.266 kb on + strand, within GFF880 at 59.267 kb on - strand, within GFF880 at 59.267 kb on - strand, within GFF880 at 59.307 kb on + strand, within GFF880 at 59.364 kb on + strand, within GFF880 at 59.365 kb on - strand, within GFF880 at 59.374 kb on - strand, within GFF880 at 59.533 kb on - strand, within GFF880 at 59.533 kb on - strand, within GFF880 at 59.603 kb on - strand, within GFF880 at 59.825 kb on - strand at 60.141 kb on + strand at 60.142 kb on - strand at 60.177 kb on - strand, within GFF881 at 60.283 kb on + strand, within GFF881 at 60.283 kb on + strand, within GFF881 at 60.284 kb on - strand, within GFF881 at 60.504 kb on - strand, within GFF881 at 60.999 kb on - strand, within GFF881 at 61.106 kb on - strand, within GFF881 at 61.126 kb on - strand, within GFF881 at 61.230 kb on + strand, within GFF881 at 61.261 kb on + strand, within GFF881 at 61.395 kb on - strand at 61.395 kb on - strand at 61.439 kb on - strand, within GFF882 at 61.534 kb on + strand, within GFF882 at 61.563 kb on - strand, within GFF882 at 61.566 kb on - strand, within GFF882 at 61.570 kb on + strand, within GFF882 at 61.646 kb on - strand at 61.646 kb on - strand at 61.711 kb on - strand at 61.794 kb on - strand at 61.849 kb on - strand at 61.895 kb on + strand at 61.983 kb on + strand, within GFF883 at 62.000 kb on + strand, within GFF883 at 62.000 kb on + strand, within GFF883 at 62.001 kb on - strand, within GFF883 at 62.003 kb on + strand, within GFF883 at 62.004 kb on - strand, within GFF883 at 62.023 kb on + strand, within GFF883 at 62.103 kb on - strand, within GFF883 at 62.154 kb on + strand, within GFF883 at 62.263 kb on + strand, within GFF883 at 62.343 kb on + strand, within GFF883 at 62.383 kb on - strand, within GFF883
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 59,077 - GFF880 0.23 -1.4 59,130 + GFF880 0.28 -0.0 59,142 + GFF880 0.30 -0.1 59,217 + GFF880 0.37 -0.1 59,222 - GFF880 0.38 +0.2 59,266 + GFF880 0.42 -0.8 59,266 + GFF880 0.42 -0.0 59,266 + GFF880 0.42 -0.3 59,267 - GFF880 0.42 -1.3 59,267 - GFF880 0.42 -0.4 59,307 + GFF880 0.46 -0.8 59,364 + GFF880 0.52 -1.8 59,365 - GFF880 0.52 +0.3 59,374 - GFF880 0.53 -0.2 59,533 - GFF880 0.69 +0.7 59,533 - GFF880 0.69 +0.1 59,603 - GFF880 0.76 -0.4 59,825 - +0.3 60,141 + +0.1 60,142 - -1.6 60,177 - GFF881 0.11 -0.9 60,283 + GFF881 0.19 -0.7 60,283 + GFF881 0.19 +0.3 60,284 - GFF881 0.19 -0.2 60,504 - GFF881 0.35 -1.4 60,999 - GFF881 0.71 -1.3 61,106 - GFF881 0.79 +0.4 61,126 - GFF881 0.80 -1.7 61,230 + GFF881 0.88 -2.8 61,261 + GFF881 0.90 +0.6 61,395 - -0.2 61,395 - -0.8 61,439 - GFF882 0.16 +0.7 61,534 + GFF882 0.53 -1.7 61,563 - GFF882 0.64 +0.3 61,566 - GFF882 0.65 +0.5 61,570 + GFF882 0.67 -0.1 61,646 - +0.0 61,646 - +0.2 61,711 - +0.1 61,794 - -0.7 61,849 - -1.6 61,895 + -0.0 61,983 + GFF883 0.11 -2.0 62,000 + GFF883 0.13 -0.7 62,000 + GFF883 0.13 -1.3 62,001 - GFF883 0.13 -0.4 62,003 + GFF883 0.14 -0.7 62,004 - GFF883 0.14 -1.2 62,023 + GFF883 0.16 -1.1 62,103 - GFF883 0.26 -1.9 62,154 + GFF883 0.32 -1.5 62,263 + GFF883 0.45 -0.4 62,343 + GFF883 0.54 -1.1 62,383 - GFF883 0.59 -1.9
Or see this region's nucleotide sequence