Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF820 and GFF821 overlap by 26 nucleotides GFF821 and GFF822 are separated by 16 nucleotides GFF822 and GFF823 are separated by 9 nucleotides
GFF820 - NAD(P)HX epimerase / NAD(P)HX dehydratase, at 1,720 to 3,264
GFF820
GFF821 - TsaE protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA, at 3,239 to 3,697
GFF821
GFF822 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 3,714 to 5,033
GFF822
GFF823 - DNA mismatch repair protein MutL, at 5,043 to 6,899
GFF823
Position (kb) 3
4
5
6 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2.763 kb on + strand, within GFF820 at 2.763 kb on + strand, within GFF820 at 2.763 kb on + strand, within GFF820 at 2.763 kb on + strand, within GFF820 at 2.901 kb on - strand, within GFF820 at 2.922 kb on - strand, within GFF820 at 3.230 kb on + strand at 3.699 kb on + strand at 3.699 kb on + strand at 3.700 kb on - strand at 3.742 kb on + strand at 3.751 kb on + strand at 3.752 kb on - strand at 3.830 kb on + strand at 3.865 kb on - strand, within GFF822 at 4.068 kb on + strand, within GFF822 at 4.069 kb on - strand, within GFF822 at 4.069 kb on - strand, within GFF822 at 4.372 kb on + strand, within GFF822 at 4.382 kb on - strand, within GFF822 at 4.382 kb on - strand, within GFF822 at 4.389 kb on - strand, within GFF822 at 4.403 kb on + strand, within GFF822 at 4.621 kb on + strand, within GFF822 at 4.666 kb on - strand, within GFF822 at 4.719 kb on + strand, within GFF822 at 4.720 kb on - strand, within GFF822 at 4.720 kb on - strand, within GFF822 at 4.739 kb on - strand, within GFF822 at 4.739 kb on - strand, within GFF822 at 4.794 kb on - strand, within GFF822 at 4.916 kb on + strand at 4.917 kb on - strand at 4.917 kb on - strand at 4.918 kb on + strand at 4.919 kb on - strand at 4.921 kb on - strand at 5.202 kb on - strand at 5.309 kb on + strand, within GFF823 at 5.372 kb on - strand, within GFF823 at 5.497 kb on - strand, within GFF823 at 5.502 kb on - strand, within GFF823 at 5.546 kb on + strand, within GFF823 at 5.812 kb on - strand, within GFF823 at 6.009 kb on + strand, within GFF823 at 6.009 kb on + strand, within GFF823 at 6.010 kb on - strand, within GFF823 at 6.010 kb on - strand, within GFF823
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 2,763 + GFF820 0.68 -0.1 2,763 + GFF820 0.68 -1.2 2,763 + GFF820 0.68 -0.3 2,763 + GFF820 0.68 -0.2 2,901 - GFF820 0.76 +0.4 2,922 - GFF820 0.78 +0.5 3,230 + -0.4 3,699 + -0.1 3,699 + -0.0 3,700 - -0.0 3,742 + +0.4 3,751 + +0.0 3,752 - -1.4 3,830 + +0.4 3,865 - GFF822 0.11 -1.0 4,068 + GFF822 0.27 +0.5 4,069 - GFF822 0.27 -1.2 4,069 - GFF822 0.27 -0.2 4,372 + GFF822 0.50 +0.3 4,382 - GFF822 0.51 +0.2 4,382 - GFF822 0.51 -0.1 4,389 - GFF822 0.51 +0.3 4,403 + GFF822 0.52 -2.4 4,621 + GFF822 0.69 +1.3 4,666 - GFF822 0.72 +0.0 4,719 + GFF822 0.76 +0.4 4,720 - GFF822 0.76 +0.6 4,720 - GFF822 0.76 +0.3 4,739 - GFF822 0.78 -1.6 4,739 - GFF822 0.78 +1.2 4,794 - GFF822 0.82 -0.4 4,916 + +0.1 4,917 - +0.2 4,917 - +0.8 4,918 + +0.1 4,919 - +0.2 4,921 - +0.5 5,202 - -0.2 5,309 + GFF823 0.14 +0.4 5,372 - GFF823 0.18 +0.6 5,497 - GFF823 0.24 -0.0 5,502 - GFF823 0.25 -1.0 5,546 + GFF823 0.27 -1.1 5,812 - GFF823 0.41 +1.8 6,009 + GFF823 0.52 +0.1 6,009 + GFF823 0.52 -0.2 6,010 - GFF823 0.52 -1.4 6,010 - GFF823 0.52 -0.1
Or see this region's nucleotide sequence