Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF614 and GFF615 are separated by 74 nucleotides GFF615 and GFF616 are separated by 53 nucleotides GFF616 and GFF617 are separated by 191 nucleotides
GFF614 - Osmolarity sensory histidine kinase EnvZ, at 353,479 to 354,822
GFF614
GFF615 - Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49), at 354,897 to 356,516
GFF615
GFF616 - hypothetical protein, at 356,570 to 356,704
GFF616
GFF617 - Putative transport protein, at 356,896 to 358,605
GFF617
Position (kb)
354
355
356
357 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 354.012 kb on - strand, within GFF614 at 354.056 kb on + strand, within GFF614 at 354.070 kb on - strand, within GFF614 at 354.135 kb on + strand, within GFF614 at 354.321 kb on + strand, within GFF614 at 354.322 kb on - strand, within GFF614 at 354.426 kb on + strand, within GFF614 at 354.499 kb on - strand, within GFF614 at 354.660 kb on + strand, within GFF614 at 354.820 kb on + strand at 354.820 kb on + strand at 354.821 kb on - strand at 354.833 kb on + strand at 354.833 kb on + strand at 354.833 kb on + strand at 354.833 kb on + strand at 354.836 kb on + strand at 354.836 kb on + strand at 354.947 kb on + strand at 355.021 kb on + strand at 355.157 kb on + strand, within GFF615 at 355.172 kb on + strand, within GFF615 at 355.173 kb on - strand, within GFF615 at 355.211 kb on - strand, within GFF615 at 355.235 kb on + strand, within GFF615 at 355.236 kb on - strand, within GFF615 at 355.258 kb on + strand, within GFF615 at 355.258 kb on + strand, within GFF615 at 355.259 kb on - strand, within GFF615 at 355.352 kb on + strand, within GFF615 at 355.504 kb on + strand, within GFF615 at 355.504 kb on + strand, within GFF615 at 355.505 kb on - strand, within GFF615 at 355.839 kb on - strand, within GFF615 at 355.858 kb on + strand, within GFF615 at 355.925 kb on + strand, within GFF615 at 356.018 kb on + strand, within GFF615 at 356.240 kb on + strand, within GFF615 at 356.279 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.307 kb on - strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.312 kb on + strand, within GFF615 at 356.370 kb on - strand at 356.373 kb on + strand at 356.373 kb on + strand at 356.390 kb on - strand at 356.408 kb on + strand at 356.423 kb on - strand at 356.434 kb on + strand at 356.476 kb on + strand at 356.477 kb on - strand at 356.538 kb on + strand at 356.539 kb on - strand at 356.539 kb on - strand at 356.539 kb on - strand at 356.540 kb on + strand at 356.593 kb on + strand, within GFF616 at 356.600 kb on - strand, within GFF616 at 356.736 kb on + strand at 356.737 kb on - strand at 356.761 kb on + strand at 356.919 kb on + strand at 356.920 kb on - strand at 356.922 kb on + strand at 356.922 kb on + strand at 356.971 kb on + strand at 356.972 kb on - strand at 356.972 kb on - strand at 357.003 kb on + strand at 357.020 kb on - strand at 357.020 kb on - strand at 357.020 kb on - strand at 357.024 kb on + strand at 357.057 kb on - strand at 357.057 kb on - strand at 357.064 kb on - strand at 357.078 kb on + strand, within GFF617 at 357.202 kb on + strand, within GFF617 at 357.253 kb on + strand, within GFF617 at 357.320 kb on + strand, within GFF617 at 357.320 kb on + strand, within GFF617 at 357.448 kb on - strand, within GFF617 at 357.452 kb on - strand, within GFF617
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 354,012 - GFF614 0.40 +1.0 354,056 + GFF614 0.43 +0.9 354,070 - GFF614 0.44 +0.8 354,135 + GFF614 0.49 +2.2 354,321 + GFF614 0.63 +1.6 354,322 - GFF614 0.63 +1.6 354,426 + GFF614 0.70 +1.2 354,499 - GFF614 0.76 +1.1 354,660 + GFF614 0.88 +0.6 354,820 + +0.2 354,820 + -0.6 354,821 - -0.2 354,833 + +1.2 354,833 + +0.0 354,833 + +0.4 354,833 + +0.4 354,836 + -0.2 354,836 + +1.4 354,947 + +0.1 355,021 + -0.5 355,157 + GFF615 0.16 -0.4 355,172 + GFF615 0.17 +0.2 355,173 - GFF615 0.17 +1.2 355,211 - GFF615 0.19 +0.4 355,235 + GFF615 0.21 +0.1 355,236 - GFF615 0.21 -0.1 355,258 + GFF615 0.22 -0.1 355,258 + GFF615 0.22 -0.8 355,259 - GFF615 0.22 -1.8 355,352 + GFF615 0.28 +0.1 355,504 + GFF615 0.37 +0.2 355,504 + GFF615 0.37 +0.2 355,505 - GFF615 0.38 -1.3 355,839 - GFF615 0.58 +0.4 355,858 + GFF615 0.59 -0.5 355,925 + GFF615 0.63 -0.0 356,018 + GFF615 0.69 +0.3 356,240 + GFF615 0.83 -0.2 356,279 + GFF615 0.85 -0.0 356,306 + GFF615 0.87 -0.3 356,306 + GFF615 0.87 -0.1 356,306 + GFF615 0.87 +0.0 356,307 - GFF615 0.87 +0.4 356,308 + GFF615 0.87 -0.2 356,308 + GFF615 0.87 -0.5 356,308 + GFF615 0.87 -0.6 356,308 + GFF615 0.87 -1.0 356,309 - GFF615 0.87 -0.6 356,309 - GFF615 0.87 -1.1 356,309 - GFF615 0.87 -0.3 356,309 - GFF615 0.87 +0.2 356,312 + GFF615 0.87 +0.2 356,370 - +0.1 356,373 + -0.3 356,373 + -0.7 356,390 - +0.2 356,408 + +0.1 356,423 - +0.7 356,434 + +0.2 356,476 + +0.5 356,477 - +0.2 356,538 + -0.3 356,539 - +0.1 356,539 - +0.1 356,539 - +0.4 356,540 + +0.0 356,593 + GFF616 0.17 +0.5 356,600 - GFF616 0.22 -1.2 356,736 + +0.5 356,737 - -0.1 356,761 + -1.9 356,919 + +0.1 356,920 - +0.1 356,922 + -0.5 356,922 + -0.2 356,971 + -0.7 356,972 - +0.5 356,972 - -0.6 357,003 + -0.2 357,020 - -0.4 357,020 - -0.6 357,020 - -0.0 357,024 + +0.1 357,057 - +0.2 357,057 - +0.0 357,064 - +0.4 357,078 + GFF617 0.11 +0.4 357,202 + GFF617 0.18 +0.2 357,253 + GFF617 0.21 -0.2 357,320 + GFF617 0.25 -0.2 357,320 + GFF617 0.25 -0.1 357,448 - GFF617 0.32 +0.2 357,452 - GFF617 0.33 +0.7
Or see this region's nucleotide sequence