Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4799 and GFF4800 are separated by 9 nucleotides GFF4800 and GFF4801 are separated by 250 nucleotides
GFF4799 - Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4), at 102,725 to 104,164
GFF4799
GFF4800 - Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4), at 104,174 to 105,082
GFF4800
GFF4801 - Alkaline phosphatase isozyme conversion protein precursor (EC 3.4.11.-), at 105,333 to 106,379
GFF4801
Position (kb) 104
105
106 Strain fitness (log2 ratio) -1
0
1
2 at 103.232 kb on + strand, within GFF4799 at 103.411 kb on - strand, within GFF4799 at 103.443 kb on - strand, within GFF4799 at 103.454 kb on + strand, within GFF4799 at 103.455 kb on - strand, within GFF4799 at 103.460 kb on + strand, within GFF4799 at 103.706 kb on - strand, within GFF4799 at 103.711 kb on + strand, within GFF4799 at 103.796 kb on - strand, within GFF4799 at 103.804 kb on + strand, within GFF4799 at 103.804 kb on + strand, within GFF4799 at 103.805 kb on - strand, within GFF4799 at 103.837 kb on + strand, within GFF4799 at 103.882 kb on + strand, within GFF4799 at 103.886 kb on - strand, within GFF4799 at 103.919 kb on + strand, within GFF4799 at 103.976 kb on - strand, within GFF4799 at 104.153 kb on + strand at 104.154 kb on - strand at 104.176 kb on - strand at 104.182 kb on - strand at 104.494 kb on - strand, within GFF4800 at 104.572 kb on - strand, within GFF4800 at 104.727 kb on - strand, within GFF4800 at 104.812 kb on + strand, within GFF4800 at 104.813 kb on - strand, within GFF4800 at 104.816 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.865 kb on + strand, within GFF4800 at 104.904 kb on - strand, within GFF4800 at 104.982 kb on + strand, within GFF4800 at 104.983 kb on - strand, within GFF4800 at 105.025 kb on + strand at 105.083 kb on - strand at 105.125 kb on + strand at 105.136 kb on - strand at 105.178 kb on + strand at 105.317 kb on + strand at 105.321 kb on + strand at 105.434 kb on + strand at 105.563 kb on + strand, within GFF4801 at 105.575 kb on + strand, within GFF4801 at 105.581 kb on + strand, within GFF4801 at 105.582 kb on - strand, within GFF4801 at 105.689 kb on + strand, within GFF4801 at 105.694 kb on - strand, within GFF4801 at 105.762 kb on - strand, within GFF4801 at 105.814 kb on + strand, within GFF4801 at 105.872 kb on - strand, within GFF4801 at 105.872 kb on - strand, within GFF4801 at 105.974 kb on + strand, within GFF4801
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 103,232 + GFF4799 0.35 +0.2 103,411 - GFF4799 0.48 +1.1 103,443 - GFF4799 0.50 -0.5 103,454 + GFF4799 0.51 -0.1 103,455 - GFF4799 0.51 +0.9 103,460 + GFF4799 0.51 -0.1 103,706 - GFF4799 0.68 +0.1 103,711 + GFF4799 0.68 +0.5 103,796 - GFF4799 0.74 +1.4 103,804 + GFF4799 0.75 -0.1 103,804 + GFF4799 0.75 +0.4 103,805 - GFF4799 0.75 +0.8 103,837 + GFF4799 0.77 +0.9 103,882 + GFF4799 0.80 -0.6 103,886 - GFF4799 0.81 +1.9 103,919 + GFF4799 0.83 +0.6 103,976 - GFF4799 0.87 +0.7 104,153 + +0.7 104,154 - +0.4 104,176 - +0.3 104,182 - +0.8 104,494 - GFF4800 0.35 -0.3 104,572 - GFF4800 0.44 +0.7 104,727 - GFF4800 0.61 -0.2 104,812 + GFF4800 0.70 +0.3 104,813 - GFF4800 0.70 +0.9 104,816 + GFF4800 0.71 +0.6 104,865 + GFF4800 0.76 -0.3 104,865 + GFF4800 0.76 -0.3 104,865 + GFF4800 0.76 +0.6 104,904 - GFF4800 0.80 +0.4 104,982 + GFF4800 0.89 +0.3 104,983 - GFF4800 0.89 +0.7 105,025 + +1.0 105,083 - -0.0 105,125 + +0.7 105,136 - +0.6 105,178 + +0.3 105,317 + +0.0 105,321 + -0.0 105,434 + +0.2 105,563 + GFF4801 0.22 -0.2 105,575 + GFF4801 0.23 -1.1 105,581 + GFF4801 0.24 -0.1 105,582 - GFF4801 0.24 +0.0 105,689 + GFF4801 0.34 +1.8 105,694 - GFF4801 0.34 +0.2 105,762 - GFF4801 0.41 -1.5 105,814 + GFF4801 0.46 -1.6 105,872 - GFF4801 0.51 -0.9 105,872 - GFF4801 0.51 -1.7 105,974 + GFF4801 0.61 -0.0
Or see this region's nucleotide sequence