Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF442 and GFF443 are separated by 104 nucleotides GFF443 and GFF444 are separated by 81 nucleotides GFF444 and GFF445 are separated by 99 nucleotides
GFF442 - Xylose activator XylR (AraC family), at 163,748 to 164,926
GFF442
GFF443 - FIG01045230: hypothetical protein, at 165,031 to 165,207
GFF443
GFF444 - Xylose isomerase (EC 5.3.1.5), at 165,289 to 166,611
GFF444
GFF445 - Xylulose kinase (EC 2.7.1.17), at 166,711 to 168,165
GFF445
Position (kb)
165
166
167 Strain fitness (log2 ratio)
-2
-1
0
1 at 164.462 kb on + strand, within GFF442 at 164.554 kb on - strand, within GFF442 at 164.895 kb on - strand at 164.933 kb on - strand at 164.985 kb on + strand at 165.084 kb on - strand, within GFF443 at 165.210 kb on + strand at 165.210 kb on + strand at 165.211 kb on - strand at 165.221 kb on + strand at 165.256 kb on + strand at 165.288 kb on + strand at 165.399 kb on + strand at 165.439 kb on - strand, within GFF444 at 165.463 kb on - strand, within GFF444 at 165.473 kb on + strand, within GFF444 at 165.473 kb on + strand, within GFF444 at 165.631 kb on + strand, within GFF444 at 165.631 kb on + strand, within GFF444 at 165.633 kb on + strand, within GFF444 at 165.634 kb on - strand, within GFF444 at 165.692 kb on + strand, within GFF444 at 165.822 kb on + strand, within GFF444 at 165.822 kb on + strand, within GFF444 at 165.952 kb on - strand, within GFF444 at 166.016 kb on - strand, within GFF444 at 166.158 kb on + strand, within GFF444 at 166.333 kb on - strand, within GFF444 at 166.336 kb on + strand, within GFF444 at 166.348 kb on + strand, within GFF444 at 166.520 kb on - strand at 166.533 kb on + strand at 166.650 kb on - strand at 166.710 kb on + strand at 166.710 kb on + strand at 166.710 kb on + strand at 166.711 kb on - strand at 166.711 kb on - strand at 166.711 kb on - strand at 166.714 kb on + strand at 166.714 kb on + strand at 166.715 kb on - strand at 166.792 kb on - strand at 167.105 kb on + strand, within GFF445 at 167.106 kb on - strand, within GFF445 at 167.212 kb on - strand, within GFF445 at 167.272 kb on - strand, within GFF445 at 167.291 kb on + strand, within GFF445 at 167.384 kb on + strand, within GFF445 at 167.385 kb on - strand, within GFF445 at 167.385 kb on - strand, within GFF445 at 167.433 kb on - strand, within GFF445 at 167.570 kb on + strand, within GFF445 at 167.571 kb on - strand, within GFF445
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 164,462 + GFF442 0.61 +0.1 164,554 - GFF442 0.68 +0.1 164,895 - +0.3 164,933 - +0.4 164,985 + -0.5 165,084 - GFF443 0.30 +0.6 165,210 + +0.4 165,210 + -0.3 165,211 - -0.9 165,221 + +0.1 165,256 + -0.1 165,288 + +0.0 165,399 + -1.1 165,439 - GFF444 0.11 -0.8 165,463 - GFF444 0.13 -1.0 165,473 + GFF444 0.14 +0.1 165,473 + GFF444 0.14 +0.0 165,631 + GFF444 0.26 -0.7 165,631 + GFF444 0.26 -0.1 165,633 + GFF444 0.26 +0.2 165,634 - GFF444 0.26 -0.5 165,692 + GFF444 0.30 -0.1 165,822 + GFF444 0.40 +0.1 165,822 + GFF444 0.40 +0.0 165,952 - GFF444 0.50 -0.4 166,016 - GFF444 0.55 -2.0 166,158 + GFF444 0.66 +0.0 166,333 - GFF444 0.79 -0.1 166,336 + GFF444 0.79 -0.5 166,348 + GFF444 0.80 -1.8 166,520 - -0.7 166,533 + +0.4 166,650 - -0.4 166,710 + -1.1 166,710 + -1.8 166,710 + +0.1 166,711 - +0.6 166,711 - +0.1 166,711 - -0.4 166,714 + +0.1 166,714 + +0.1 166,715 - -0.0 166,792 - -2.1 167,105 + GFF445 0.27 -0.1 167,106 - GFF445 0.27 +0.6 167,212 - GFF445 0.34 +0.5 167,272 - GFF445 0.39 -0.2 167,291 + GFF445 0.40 -0.4 167,384 + GFF445 0.46 -0.3 167,385 - GFF445 0.46 -0.4 167,385 - GFF445 0.46 -0.1 167,433 - GFF445 0.50 +0.2 167,570 + GFF445 0.59 +1.2 167,571 - GFF445 0.59 +0.0
Or see this region's nucleotide sequence