Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4407 and GFF4408 are separated by 146 nucleotides GFF4408 and GFF4409 overlap by 14 nucleotides GFF4409 and GFF4410 are separated by 85 nucleotides GFF4410 and GFF4411 are separated by 32 nucleotides
GFF4407 - Putative stomatin/prohibitin-family membrane protease subunit YbbK, at 191,636 to 192,553
GFF4407
GFF4408 - YbbL ABC transporter ATP-binding protein, at 192,700 to 193,377
GFF4408
GFF4409 - YbbM seven transmembrane helix protein, at 193,364 to 194,143
GFF4409
GFF4410 - FIG000875: Thioredoxin domain-containing protein EC-YbbN, at 194,229 to 195,110
GFF4410
GFF4411 - Putative NAD(P)-dependent oxidoreductase EC-YbbO, at 195,143 to 195,913
GFF4411
Position (kb) 193
194
195 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 192.535 kb on + strand at 192.536 kb on - strand at 192.612 kb on - strand at 192.647 kb on - strand at 192.659 kb on - strand at 192.659 kb on - strand at 192.712 kb on - strand at 192.912 kb on + strand, within GFF4408 at 192.935 kb on - strand, within GFF4408 at 192.952 kb on + strand, within GFF4408 at 192.992 kb on - strand, within GFF4408 at 192.992 kb on - strand, within GFF4408 at 193.134 kb on + strand, within GFF4408 at 193.135 kb on - strand, within GFF4408 at 193.149 kb on + strand, within GFF4408 at 193.213 kb on - strand, within GFF4408 at 193.236 kb on + strand, within GFF4408 at 193.375 kb on + strand at 193.376 kb on - strand at 193.378 kb on + strand at 193.378 kb on + strand at 193.378 kb on + strand at 193.378 kb on + strand at 193.388 kb on - strand at 193.482 kb on + strand, within GFF4409 at 193.483 kb on - strand, within GFF4409 at 193.619 kb on - strand, within GFF4409 at 193.622 kb on + strand, within GFF4409 at 193.771 kb on - strand, within GFF4409 at 193.787 kb on + strand, within GFF4409 at 193.788 kb on - strand, within GFF4409 at 193.788 kb on - strand, within GFF4409 at 193.882 kb on + strand, within GFF4409 at 194.021 kb on - strand, within GFF4409 at 194.080 kb on - strand at 194.097 kb on - strand at 194.145 kb on - strand at 194.204 kb on - strand at 194.231 kb on - strand at 194.233 kb on + strand at 194.245 kb on + strand at 194.246 kb on - strand at 194.390 kb on + strand, within GFF4410 at 194.390 kb on + strand, within GFF4410 at 194.712 kb on + strand, within GFF4410 at 194.839 kb on + strand, within GFF4410 at 194.930 kb on - strand, within GFF4410 at 194.999 kb on - strand, within GFF4410 at 195.031 kb on - strand at 195.060 kb on - strand at 195.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 192,535 + +2.1 192,536 - -0.0 192,612 - -0.2 192,647 - -0.3 192,659 - -0.0 192,659 - -0.3 192,712 - -1.2 192,912 + GFF4408 0.31 +0.7 192,935 - GFF4408 0.35 -1.1 192,952 + GFF4408 0.37 -1.6 192,992 - GFF4408 0.43 -1.1 192,992 - GFF4408 0.43 -0.8 193,134 + GFF4408 0.64 -1.0 193,135 - GFF4408 0.64 -2.8 193,149 + GFF4408 0.66 -2.2 193,213 - GFF4408 0.76 -1.6 193,236 + GFF4408 0.79 -2.5 193,375 + -1.2 193,376 - -1.0 193,378 + -0.9 193,378 + -0.1 193,378 + -3.2 193,378 + -0.2 193,388 - -0.8 193,482 + GFF4409 0.15 -1.1 193,483 - GFF4409 0.15 -1.4 193,619 - GFF4409 0.33 -1.4 193,622 + GFF4409 0.33 -0.8 193,771 - GFF4409 0.52 -0.5 193,787 + GFF4409 0.54 -0.9 193,788 - GFF4409 0.54 -0.9 193,788 - GFF4409 0.54 -1.0 193,882 + GFF4409 0.66 -0.7 194,021 - GFF4409 0.84 -0.6 194,080 - -2.4 194,097 - +1.2 194,145 - +1.2 194,204 - +1.0 194,231 - +0.0 194,233 + -0.9 194,245 + +0.9 194,246 - +0.3 194,390 + GFF4410 0.18 +0.1 194,390 + GFF4410 0.18 -0.1 194,712 + GFF4410 0.55 -1.2 194,839 + GFF4410 0.69 -1.0 194,930 - GFF4410 0.79 -0.7 194,999 - GFF4410 0.87 +0.1 195,031 - -1.6 195,060 - +0.3 195,114 + +0.1
Or see this region's nucleotide sequence