Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4361 and GFF4362 are separated by 100 nucleotides GFF4362 and GFF4363 are separated by 49 nucleotides GFF4363 and GFF4364 are separated by 112 nucleotides GFF4364 and GFF4365 are separated by 40 nucleotides
GFF4361 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 142,643 to 144,343
GFF4361
GFF4362 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 144,444 to 145,262
GFF4362
GFF4363 - Cysteine synthase B (EC 2.5.1.47), at 145,312 to 146,367
GFF4363
GFF4364 - Putative HTH-type transcriptional regulator ybaO, at 146,480 to 146,938
GFF4364
GFF4365 - ATP-binding component, at 146,979 to 148,751
GFF4365
Position (kb)
145
146
147 Strain fitness (log2 ratio)
-1
0
1 at 144.335 kb on + strand at 144.381 kb on - strand at 144.468 kb on - strand at 144.481 kb on + strand at 144.484 kb on + strand at 144.485 kb on - strand at 144.625 kb on - strand, within GFF4362 at 145.243 kb on + strand at 145.243 kb on + strand at 145.244 kb on - strand at 145.244 kb on - strand at 145.249 kb on - strand at 145.394 kb on + strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.398 kb on - strand at 145.500 kb on + strand, within GFF4363 at 145.746 kb on + strand, within GFF4363 at 145.751 kb on + strand, within GFF4363 at 145.752 kb on - strand, within GFF4363 at 145.767 kb on + strand, within GFF4363 at 145.768 kb on - strand, within GFF4363 at 145.931 kb on + strand, within GFF4363 at 145.931 kb on + strand, within GFF4363 at 145.931 kb on + strand, within GFF4363 at 145.932 kb on - strand, within GFF4363 at 146.180 kb on + strand, within GFF4363 at 146.250 kb on - strand, within GFF4363 at 146.399 kb on - strand at 146.500 kb on + strand at 146.531 kb on - strand, within GFF4364 at 146.531 kb on - strand, within GFF4364 at 146.787 kb on - strand, within GFF4364 at 146.787 kb on - strand, within GFF4364 at 146.840 kb on + strand, within GFF4364 at 146.909 kb on - strand at 146.909 kb on - strand at 146.909 kb on - strand at 146.909 kb on - strand at 146.986 kb on + strand at 147.030 kb on + strand at 147.054 kb on - strand at 147.220 kb on + strand, within GFF4365 at 147.241 kb on + strand, within GFF4365
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 144,335 + -0.7 144,381 - +0.2 144,468 - -0.1 144,481 + +0.1 144,484 + +0.2 144,485 - -0.1 144,625 - GFF4362 0.22 +0.4 145,243 + -0.2 145,243 + +0.5 145,244 - +0.3 145,244 - +0.4 145,249 - +0.8 145,394 + +0.2 145,398 - +0.6 145,398 - -0.6 145,398 - +0.3 145,398 - -0.2 145,398 - -0.2 145,500 + GFF4363 0.18 -1.5 145,746 + GFF4363 0.41 -0.5 145,751 + GFF4363 0.42 -0.2 145,752 - GFF4363 0.42 +0.3 145,767 + GFF4363 0.43 -0.4 145,768 - GFF4363 0.43 -0.4 145,931 + GFF4363 0.59 -0.5 145,931 + GFF4363 0.59 +1.1 145,931 + GFF4363 0.59 -0.6 145,932 - GFF4363 0.59 -1.0 146,180 + GFF4363 0.82 -0.5 146,250 - GFF4363 0.89 -0.1 146,399 - +1.5 146,500 + -0.6 146,531 - GFF4364 0.11 -0.4 146,531 - GFF4364 0.11 -1.2 146,787 - GFF4364 0.67 -0.4 146,787 - GFF4364 0.67 -0.5 146,840 + GFF4364 0.78 +0.9 146,909 - -0.1 146,909 - -0.1 146,909 - +0.3 146,909 - -1.0 146,986 + +0.2 147,030 + +0.5 147,054 - +0.3 147,220 + GFF4365 0.14 +0.2 147,241 + GFF4365 0.15 -0.6
Or see this region's nucleotide sequence