Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4098 and GFF4099 are separated by 370 nucleotides GFF4099 and GFF4100 are separated by 108 nucleotides
GFF4098 - Probable secreted protein, at 56,353 to 56,760
GFF4098
GFF4099 - Aliphatic amidase AmiE (EC 3.5.1.4), at 57,131 to 57,898
GFF4099
GFF4100 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 58,007 to 60,451
GFF4100
Position (kb) 57
58 Strain fitness (log2 ratio) -1
0
1 at 56.433 kb on - strand, within GFF4098 at 56.464 kb on + strand, within GFF4098 at 56.786 kb on + strand at 56.852 kb on - strand at 57.043 kb on + strand at 57.061 kb on - strand at 57.061 kb on - strand at 57.133 kb on - strand at 57.181 kb on - strand at 57.291 kb on + strand, within GFF4099 at 57.371 kb on + strand, within GFF4099 at 57.414 kb on - strand, within GFF4099 at 57.417 kb on + strand, within GFF4099 at 57.418 kb on - strand, within GFF4099 at 57.418 kb on - strand, within GFF4099 at 57.433 kb on - strand, within GFF4099 at 57.581 kb on - strand, within GFF4099 at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 57.936 kb on - strand at 58.145 kb on - strand at 58.149 kb on - strand at 58.150 kb on + strand at 58.150 kb on + strand at 58.195 kb on + strand at 58.281 kb on - strand, within GFF4100 at 58.358 kb on + strand, within GFF4100 at 58.358 kb on + strand, within GFF4100 at 58.359 kb on - strand, within GFF4100 at 58.388 kb on - strand, within GFF4100 at 58.596 kb on + strand, within GFF4100 at 58.597 kb on - strand, within GFF4100 at 58.725 kb on + strand, within GFF4100 at 58.726 kb on - strand, within GFF4100 at 58.843 kb on + strand, within GFF4100 at 58.844 kb on - strand, within GFF4100 at 58.844 kb on - strand, within GFF4100 at 58.865 kb on - strand, within GFF4100 at 58.893 kb on - strand, within GFF4100 at 58.896 kb on + strand, within GFF4100 at 58.896 kb on + strand, within GFF4100 at 58.897 kb on - strand, within GFF4100 at 58.897 kb on - strand, within GFF4100 at 58.897 kb on - strand, within GFF4100
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 56,433 - GFF4098 0.20 -0.5 56,464 + GFF4098 0.27 -0.3 56,786 + +0.5 56,852 - -0.1 57,043 + +0.1 57,061 - -0.5 57,061 - -0.1 57,133 - +0.1 57,181 - +0.2 57,291 + GFF4099 0.21 -1.7 57,371 + GFF4099 0.31 +0.4 57,414 - GFF4099 0.37 +1.0 57,417 + GFF4099 0.37 -1.0 57,418 - GFF4099 0.37 -0.9 57,418 - GFF4099 0.37 +0.1 57,433 - GFF4099 0.39 -0.4 57,581 - GFF4099 0.59 -1.1 57,936 - +0.5 57,936 - +0.4 57,936 - -0.1 57,936 - +0.2 57,936 - +0.6 57,936 - +0.0 58,145 - -0.2 58,149 - +0.4 58,150 + -0.7 58,150 + -0.8 58,195 + -1.3 58,281 - GFF4100 0.11 +0.1 58,358 + GFF4100 0.14 +0.5 58,358 + GFF4100 0.14 +0.4 58,359 - GFF4100 0.14 +0.0 58,388 - GFF4100 0.16 +0.4 58,596 + GFF4100 0.24 -0.2 58,597 - GFF4100 0.24 -0.5 58,725 + GFF4100 0.29 -0.0 58,726 - GFF4100 0.29 -0.9 58,843 + GFF4100 0.34 -0.5 58,844 - GFF4100 0.34 -0.0 58,844 - GFF4100 0.34 +0.1 58,865 - GFF4100 0.35 -0.4 58,893 - GFF4100 0.36 +0.1 58,896 + GFF4100 0.36 +0.3 58,896 + GFF4100 0.36 +0.3 58,897 - GFF4100 0.36 -0.1 58,897 - GFF4100 0.36 +0.2 58,897 - GFF4100 0.36 -0.5
Or see this region's nucleotide sequence