Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3885 and GFF3886 are separated by 177 nucleotides GFF3886 and GFF3887 are separated by 376 nucleotides
GFF3885 - Colanic acid biosynthesis protein wcaM, at 163,422 to 164,825
GFF3885
GFF3886 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 165,003 to 165,896
GFF3886
GFF3887 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 166,273 to 167,358
GFF3887
Position (kb) 165
166 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 164.075 kb on + strand, within GFF3885 at 164.082 kb on - strand, within GFF3885 at 164.134 kb on - strand, within GFF3885 at 164.146 kb on - strand, within GFF3885 at 164.410 kb on - strand, within GFF3885 at 164.437 kb on + strand, within GFF3885 at 164.472 kb on + strand, within GFF3885 at 164.666 kb on - strand, within GFF3885 at 164.666 kb on - strand, within GFF3885 at 164.765 kb on + strand at 164.869 kb on - strand at 164.898 kb on - strand at 165.023 kb on + strand at 165.050 kb on + strand at 165.050 kb on + strand at 165.050 kb on + strand at 165.050 kb on + strand at 165.051 kb on - strand at 165.051 kb on - strand at 165.051 kb on - strand at 165.117 kb on + strand, within GFF3886 at 165.118 kb on - strand, within GFF3886 at 165.118 kb on - strand, within GFF3886 at 165.213 kb on + strand, within GFF3886 at 165.213 kb on + strand, within GFF3886 at 165.275 kb on + strand, within GFF3886 at 165.341 kb on + strand, within GFF3886 at 165.341 kb on + strand, within GFF3886 at 165.342 kb on - strand, within GFF3886 at 165.435 kb on + strand, within GFF3886 at 165.435 kb on + strand, within GFF3886 at 165.436 kb on - strand, within GFF3886 at 165.437 kb on + strand, within GFF3886 at 165.783 kb on + strand, within GFF3886 at 165.784 kb on - strand, within GFF3886 at 165.874 kb on + strand at 165.875 kb on - strand at 166.085 kb on + strand at 166.158 kb on - strand at 166.585 kb on + strand, within GFF3887 at 166.612 kb on + strand, within GFF3887 at 166.764 kb on + strand, within GFF3887 at 166.825 kb on + strand, within GFF3887 at 166.857 kb on + strand, within GFF3887 at 166.860 kb on + strand, within GFF3887 at 166.864 kb on + strand, within GFF3887 at 166.864 kb on + strand, within GFF3887
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 164,075 + GFF3885 0.47 +0.4 164,082 - GFF3885 0.47 +0.1 164,134 - GFF3885 0.51 +0.2 164,146 - GFF3885 0.52 +0.2 164,410 - GFF3885 0.70 +0.3 164,437 + GFF3885 0.72 +0.2 164,472 + GFF3885 0.75 +0.1 164,666 - GFF3885 0.89 +0.4 164,666 - GFF3885 0.89 -0.2 164,765 + +1.1 164,869 - +0.2 164,898 - -0.5 165,023 + -2.2 165,050 + -1.6 165,050 + -3.1 165,050 + -1.7 165,050 + -0.9 165,051 - -4.1 165,051 - -2.5 165,051 - -3.6 165,117 + GFF3886 0.13 -0.9 165,118 - GFF3886 0.13 -1.4 165,118 - GFF3886 0.13 -3.1 165,213 + GFF3886 0.23 -2.8 165,213 + GFF3886 0.23 -1.2 165,275 + GFF3886 0.30 -1.9 165,341 + GFF3886 0.38 -0.1 165,341 + GFF3886 0.38 +0.0 165,342 - GFF3886 0.38 -4.1 165,435 + GFF3886 0.48 -0.7 165,435 + GFF3886 0.48 -0.6 165,436 - GFF3886 0.48 -3.1 165,437 + GFF3886 0.49 -1.8 165,783 + GFF3886 0.87 -0.4 165,784 - GFF3886 0.87 -2.0 165,874 + -1.0 165,875 - -1.8 166,085 + -1.0 166,158 - -1.4 166,585 + GFF3887 0.29 -4.5 166,612 + GFF3887 0.31 -5.7 166,764 + GFF3887 0.45 -3.5 166,825 + GFF3887 0.51 -2.5 166,857 + GFF3887 0.54 -2.8 166,860 + GFF3887 0.54 -1.9 166,864 + GFF3887 0.54 -2.5 166,864 + GFF3887 0.54 -3.8
Or see this region's nucleotide sequence