Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3795 and GFF3796 are separated by 153 nucleotides GFF3796 and GFF3797 overlap by 4 nucleotides GFF3797 and GFF3798 are separated by 131 nucleotides
GFF3795 - Cytidine deaminase (EC 3.5.4.5), at 64,802 to 65,686
GFF3795
GFF3796 - LrgA-associated membrane protein LrgB, at 65,840 to 66,535
GFF3796
GFF3797 - Antiholin-like protein LrgA, at 66,532 to 66,930
GFF3797
GFF3798 - Putative transcriptional regulator, at 67,062 to 67,970
GFF3798
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1
2 at 65.069 kb on - strand, within GFF3795 at 65.078 kb on - strand, within GFF3795 at 65.078 kb on - strand, within GFF3795 at 65.086 kb on + strand, within GFF3795 at 65.135 kb on - strand, within GFF3795 at 65.221 kb on + strand, within GFF3795 at 65.222 kb on - strand, within GFF3795 at 65.516 kb on + strand, within GFF3795 at 65.708 kb on - strand at 65.723 kb on + strand at 65.742 kb on - strand at 65.742 kb on - strand at 65.806 kb on - strand at 65.894 kb on - strand at 66.020 kb on - strand, within GFF3796 at 66.149 kb on - strand, within GFF3796 at 66.260 kb on - strand, within GFF3796 at 66.260 kb on - strand, within GFF3796 at 66.382 kb on - strand, within GFF3796 at 66.413 kb on - strand, within GFF3796 at 66.413 kb on - strand, within GFF3796 at 66.523 kb on + strand at 66.524 kb on - strand at 66.526 kb on - strand at 66.577 kb on + strand, within GFF3797 at 66.599 kb on - strand, within GFF3797 at 66.668 kb on - strand, within GFF3797 at 66.722 kb on + strand, within GFF3797 at 66.787 kb on + strand, within GFF3797 at 66.800 kb on - strand, within GFF3797 at 66.800 kb on - strand, within GFF3797 at 66.800 kb on - strand, within GFF3797 at 66.935 kb on - strand at 67.012 kb on + strand at 67.021 kb on + strand at 67.021 kb on + strand at 67.021 kb on + strand at 67.215 kb on + strand, within GFF3798 at 67.227 kb on - strand, within GFF3798 at 67.235 kb on + strand, within GFF3798 at 67.335 kb on - strand, within GFF3798 at 67.335 kb on - strand, within GFF3798
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 65,069 - GFF3795 0.30 +0.6 65,078 - GFF3795 0.31 +0.9 65,078 - GFF3795 0.31 -0.1 65,086 + GFF3795 0.32 -0.8 65,135 - GFF3795 0.38 +0.0 65,221 + GFF3795 0.47 -0.2 65,222 - GFF3795 0.47 +0.8 65,516 + GFF3795 0.81 -0.8 65,708 - -0.3 65,723 + +0.6 65,742 - +0.8 65,742 - +2.6 65,806 - +0.1 65,894 - +0.0 66,020 - GFF3796 0.26 +0.5 66,149 - GFF3796 0.44 +0.4 66,260 - GFF3796 0.60 +0.2 66,260 - GFF3796 0.60 -0.5 66,382 - GFF3796 0.78 +0.2 66,413 - GFF3796 0.82 +0.2 66,413 - GFF3796 0.82 +0.3 66,523 + -1.1 66,524 - +0.2 66,526 - +0.2 66,577 + GFF3797 0.11 +0.4 66,599 - GFF3797 0.17 +0.3 66,668 - GFF3797 0.34 +0.3 66,722 + GFF3797 0.48 -0.1 66,787 + GFF3797 0.64 +0.2 66,800 - GFF3797 0.67 +0.2 66,800 - GFF3797 0.67 +0.3 66,800 - GFF3797 0.67 -0.4 66,935 - +0.2 67,012 + +1.0 67,021 + +0.9 67,021 + +0.7 67,021 + +0.3 67,215 + GFF3798 0.17 +0.3 67,227 - GFF3798 0.18 +0.1 67,235 + GFF3798 0.19 +0.3 67,335 - GFF3798 0.30 -0.1 67,335 - GFF3798 0.30 +0.1
Or see this region's nucleotide sequence